Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gspE and gspF are separated by 3 nucleotides gspF and gspG are separated by 74 nucleotides gspG and gspH are separated by 1 nucleotides
SO0167: gspE - general secretion pathway protein E (NCBI ptt file), at 174,412 to 175,977
gspE
SO0168: gspF - general secretion pathway protein F (NCBI ptt file), at 175,981 to 177,204
gspF
SO0169: gspG - general secretion pathway protein G (NCBI ptt file), at 177,279 to 177,713
gspG
SO0170: gspH - general secretion pathway protein H (NCBI ptt file), at 177,715 to 178,287
gspH
Position (kb) 175
176
177
178 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 174.999 kb on + strand, within gspE at 174.999 kb on + strand, within gspE at 174.999 kb on + strand, within gspE at 175.007 kb on - strand, within gspE at 175.013 kb on - strand, within gspE at 175.269 kb on + strand, within gspE at 175.285 kb on - strand, within gspE at 175.407 kb on - strand, within gspE at 175.451 kb on - strand, within gspE at 175.484 kb on - strand, within gspE at 175.484 kb on - strand, within gspE at 175.550 kb on - strand, within gspE at 175.570 kb on + strand, within gspE at 175.578 kb on + strand, within gspE at 175.710 kb on + strand, within gspE at 175.817 kb on + strand, within gspE at 175.951 kb on + strand at 175.959 kb on - strand at 175.972 kb on + strand at 175.979 kb on - strand at 175.980 kb on - strand at 175.980 kb on - strand at 176.005 kb on - strand at 176.010 kb on + strand at 176.025 kb on + strand at 176.033 kb on - strand at 176.033 kb on - strand at 176.134 kb on + strand, within gspF at 176.229 kb on - strand, within gspF at 176.247 kb on + strand, within gspF at 176.247 kb on + strand, within gspF at 176.255 kb on - strand, within gspF at 176.255 kb on - strand, within gspF at 176.255 kb on - strand, within gspF at 176.255 kb on - strand, within gspF at 176.255 kb on - strand, within gspF at 176.260 kb on + strand, within gspF at 176.289 kb on - strand, within gspF at 176.327 kb on + strand, within gspF at 176.327 kb on + strand, within gspF at 176.335 kb on - strand, within gspF at 176.382 kb on - strand, within gspF at 176.382 kb on - strand, within gspF at 176.524 kb on - strand, within gspF at 176.555 kb on - strand, within gspF at 176.597 kb on - strand, within gspF at 176.640 kb on - strand, within gspF at 176.795 kb on - strand, within gspF at 176.834 kb on + strand, within gspF at 176.834 kb on + strand, within gspF at 176.936 kb on + strand, within gspF at 176.941 kb on + strand, within gspF at 176.941 kb on + strand, within gspF at 176.941 kb on + strand, within gspF at 176.953 kb on + strand, within gspF at 176.958 kb on + strand, within gspF at 176.958 kb on + strand, within gspF at 176.966 kb on - strand, within gspF at 176.966 kb on - strand, within gspF at 177.010 kb on - strand, within gspF at 177.118 kb on + strand at 177.269 kb on + strand at 177.277 kb on - strand at 177.303 kb on + strand at 177.303 kb on + strand at 177.329 kb on + strand, within gspG at 177.364 kb on - strand, within gspG at 177.366 kb on - strand, within gspG at 177.397 kb on - strand, within gspG at 177.476 kb on + strand, within gspG at 177.492 kb on + strand, within gspG at 177.554 kb on + strand, within gspG at 177.613 kb on - strand, within gspG at 177.638 kb on + strand, within gspG at 177.662 kb on + strand, within gspG at 177.751 kb on - strand at 177.788 kb on + strand, within gspH at 177.837 kb on + strand, within gspH at 177.837 kb on + strand, within gspH at 177.837 kb on + strand, within gspH at 177.845 kb on - strand, within gspH at 177.845 kb on - strand, within gspH at 178.065 kb on - strand, within gspH
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 174,999 + gspE SO0167 0.37 -1.2 174,999 + gspE SO0167 0.37 -1.8 174,999 + gspE SO0167 0.37 -3.2 175,007 - gspE SO0167 0.38 -1.0 175,013 - gspE SO0167 0.38 -1.5 175,269 + gspE SO0167 0.55 -1.1 175,285 - gspE SO0167 0.56 -1.0 175,407 - gspE SO0167 0.64 -1.6 175,451 - gspE SO0167 0.66 -1.4 175,484 - gspE SO0167 0.68 -2.3 175,484 - gspE SO0167 0.68 -1.9 175,550 - gspE SO0167 0.73 -1.9 175,570 + gspE SO0167 0.74 -2.5 175,578 + gspE SO0167 0.74 -2.7 175,710 + gspE SO0167 0.83 -1.8 175,817 + gspE SO0167 0.90 +0.4 175,951 + -1.0 175,959 - -2.1 175,972 + -0.4 175,979 - -1.4 175,980 - -0.3 175,980 - -0.2 176,005 - -1.5 176,010 + -0.1 176,025 + -0.9 176,033 - -1.9 176,033 - -2.3 176,134 + gspF SO0168 0.12 -1.6 176,229 - gspF SO0168 0.20 -0.4 176,247 + gspF SO0168 0.22 -2.6 176,247 + gspF SO0168 0.22 -0.6 176,255 - gspF SO0168 0.22 -1.9 176,255 - gspF SO0168 0.22 -2.1 176,255 - gspF SO0168 0.22 -1.7 176,255 - gspF SO0168 0.22 +0.6 176,255 - gspF SO0168 0.22 -1.4 176,260 + gspF SO0168 0.23 -0.1 176,289 - gspF SO0168 0.25 -2.0 176,327 + gspF SO0168 0.28 -1.1 176,327 + gspF SO0168 0.28 -3.0 176,335 - gspF SO0168 0.29 -1.8 176,382 - gspF SO0168 0.33 -0.7 176,382 - gspF SO0168 0.33 -1.8 176,524 - gspF SO0168 0.44 -2.1 176,555 - gspF SO0168 0.47 -1.1 176,597 - gspF SO0168 0.50 -1.2 176,640 - gspF SO0168 0.54 -2.1 176,795 - gspF SO0168 0.67 -0.6 176,834 + gspF SO0168 0.70 -1.2 176,834 + gspF SO0168 0.70 -1.6 176,936 + gspF SO0168 0.78 -1.4 176,941 + gspF SO0168 0.78 -1.6 176,941 + gspF SO0168 0.78 -1.1 176,941 + gspF SO0168 0.78 -0.6 176,953 + gspF SO0168 0.79 -2.0 176,958 + gspF SO0168 0.80 +0.1 176,958 + gspF SO0168 0.80 -1.5 176,966 - gspF SO0168 0.80 -1.9 176,966 - gspF SO0168 0.80 -1.7 177,010 - gspF SO0168 0.84 -2.1 177,118 + +0.6 177,269 + -0.3 177,277 - -1.1 177,303 + -2.7 177,303 + -1.5 177,329 + gspG SO0169 0.11 -1.4 177,364 - gspG SO0169 0.20 -1.0 177,366 - gspG SO0169 0.20 -0.2 177,397 - gspG SO0169 0.27 -0.3 177,476 + gspG SO0169 0.45 -2.6 177,492 + gspG SO0169 0.49 -2.0 177,554 + gspG SO0169 0.63 -1.2 177,613 - gspG SO0169 0.77 -0.8 177,638 + gspG SO0169 0.83 -0.9 177,662 + gspG SO0169 0.88 -1.3 177,751 - -1.4 177,788 + gspH SO0170 0.13 -1.6 177,837 + gspH SO0170 0.21 -1.7 177,837 + gspH SO0170 0.21 -0.2 177,837 + gspH SO0170 0.21 -1.4 177,845 - gspH SO0170 0.23 -1.1 177,845 - gspH SO0170 0.23 -1.8 178,065 - gspH SO0170 0.61 -1.8
Or see this region's nucleotide sequence