Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1364 and SO1365 are separated by 122 nucleotides SO1365 and SO1366 are separated by 45 nucleotides SO1366 and pheA are separated by 94 nucleotides
SO1364: SO1364 - iron-sulfur cluster-binding protein (NCBI ptt file), at 1,420,073 to 1,421,050
SO1364
SO1365: SO1365 - hypothetical protein (NCBI ptt file), at 1,421,173 to 1,421,508
SO1365
SO1366: SO1366 - sodium/hydrogen exchanger family protein (NCBI ptt file), at 1,421,554 to 1,422,858
SO1366
SO1367: pheA - chorismate mutase/prephenate dehydratase (NCBI ptt file), at 1,422,953 to 1,424,968
pheA
Position (kb)
1421
1422
1423 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1420.632 kb on + strand, within SO1364 at 1420.680 kb on + strand, within SO1364 at 1420.696 kb on + strand, within SO1364 at 1420.743 kb on + strand, within SO1364 at 1420.743 kb on + strand, within SO1364 at 1420.788 kb on + strand, within SO1364 at 1420.802 kb on - strand, within SO1364 at 1420.825 kb on + strand, within SO1364 at 1420.833 kb on - strand, within SO1364 at 1420.865 kb on + strand, within SO1364 at 1420.909 kb on - strand, within SO1364 at 1420.997 kb on + strand at 1421.005 kb on - strand at 1421.116 kb on + strand at 1421.116 kb on + strand at 1421.125 kb on - strand at 1421.146 kb on + strand at 1421.316 kb on + strand, within SO1365 at 1421.324 kb on - strand, within SO1365 at 1421.392 kb on + strand, within SO1365 at 1421.495 kb on + strand at 1421.495 kb on + strand at 1421.495 kb on + strand at 1421.503 kb on - strand at 1421.530 kb on + strand at 1421.583 kb on - strand at 1421.598 kb on + strand at 1421.675 kb on + strand at 1421.737 kb on + strand, within SO1366 at 1421.769 kb on + strand, within SO1366 at 1421.772 kb on - strand, within SO1366 at 1421.918 kb on - strand, within SO1366 at 1421.923 kb on - strand, within SO1366 at 1421.938 kb on + strand, within SO1366 at 1421.946 kb on - strand, within SO1366 at 1422.016 kb on + strand, within SO1366 at 1422.031 kb on + strand, within SO1366 at 1422.039 kb on - strand, within SO1366 at 1422.039 kb on - strand, within SO1366 at 1422.039 kb on - strand, within SO1366 at 1422.090 kb on + strand, within SO1366 at 1422.185 kb on - strand, within SO1366 at 1422.192 kb on + strand, within SO1366 at 1422.195 kb on - strand, within SO1366 at 1422.407 kb on - strand, within SO1366 at 1422.458 kb on - strand, within SO1366 at 1422.517 kb on - strand, within SO1366 at 1422.586 kb on - strand, within SO1366 at 1422.715 kb on - strand, within SO1366 at 1422.715 kb on - strand, within SO1366 at 1422.851 kb on + strand at 1423.013 kb on - strand at 1423.047 kb on + strand at 1423.055 kb on - strand at 1423.057 kb on + strand at 1423.140 kb on + strand at 1423.148 kb on - strand at 1423.150 kb on + strand at 1423.150 kb on + strand at 1423.158 kb on - strand, within pheA at 1423.158 kb on - strand, within pheA at 1423.166 kb on + strand, within pheA at 1423.166 kb on + strand, within pheA at 1423.323 kb on + strand, within pheA at 1423.390 kb on + strand, within pheA at 1423.471 kb on + strand, within pheA at 1423.471 kb on + strand, within pheA at 1423.479 kb on - strand, within pheA at 1423.655 kb on + strand, within pheA at 1423.663 kb on - strand, within pheA at 1423.718 kb on - strand, within pheA at 1423.741 kb on + strand, within pheA at 1423.741 kb on + strand, within pheA at 1423.816 kb on + strand, within pheA at 1423.816 kb on + strand, within pheA at 1423.824 kb on - strand, within pheA at 1423.824 kb on - strand, within pheA at 1423.855 kb on - strand, within pheA
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 1,420,632 + SO1364 0.57 -0.2 1,420,680 + SO1364 0.62 -0.3 1,420,696 + SO1364 0.64 +0.7 1,420,743 + SO1364 0.69 +0.2 1,420,743 + SO1364 0.69 -0.1 1,420,788 + SO1364 0.73 +0.5 1,420,802 - SO1364 0.75 +0.9 1,420,825 + SO1364 0.77 -0.2 1,420,833 - SO1364 0.78 -0.5 1,420,865 + SO1364 0.81 +0.3 1,420,909 - SO1364 0.85 +0.2 1,420,997 + +0.0 1,421,005 - -0.3 1,421,116 + -0.3 1,421,116 + -1.1 1,421,125 - +0.1 1,421,146 + -0.8 1,421,316 + SO1365 0.43 -0.3 1,421,324 - SO1365 0.45 +0.4 1,421,392 + SO1365 0.65 -0.3 1,421,495 + +0.2 1,421,495 + -0.1 1,421,495 + -0.5 1,421,503 - -0.7 1,421,530 + +0.0 1,421,583 - +0.7 1,421,598 + -0.0 1,421,675 + +0.7 1,421,737 + SO1366 0.14 +0.0 1,421,769 + SO1366 0.16 -0.4 1,421,772 - SO1366 0.17 -0.0 1,421,918 - SO1366 0.28 +0.5 1,421,923 - SO1366 0.28 +1.8 1,421,938 + SO1366 0.29 -0.2 1,421,946 - SO1366 0.30 -0.2 1,422,016 + SO1366 0.35 +0.3 1,422,031 + SO1366 0.37 +0.2 1,422,039 - SO1366 0.37 +0.5 1,422,039 - SO1366 0.37 +0.3 1,422,039 - SO1366 0.37 -0.7 1,422,090 + SO1366 0.41 +0.1 1,422,185 - SO1366 0.48 -0.1 1,422,192 + SO1366 0.49 +0.4 1,422,195 - SO1366 0.49 +0.0 1,422,407 - SO1366 0.65 -0.1 1,422,458 - SO1366 0.69 -0.2 1,422,517 - SO1366 0.74 +0.3 1,422,586 - SO1366 0.79 +0.3 1,422,715 - SO1366 0.89 +0.0 1,422,715 - SO1366 0.89 +0.4 1,422,851 + +0.3 1,423,013 - -0.3 1,423,047 + -0.3 1,423,055 - -0.4 1,423,057 + -0.2 1,423,140 + -0.5 1,423,148 - -0.5 1,423,150 + +0.1 1,423,150 + -0.2 1,423,158 - pheA SO1367 0.10 -0.3 1,423,158 - pheA SO1367 0.10 -0.2 1,423,166 + pheA SO1367 0.11 -0.8 1,423,166 + pheA SO1367 0.11 -1.2 1,423,323 + pheA SO1367 0.18 -2.0 1,423,390 + pheA SO1367 0.22 -0.5 1,423,471 + pheA SO1367 0.26 -0.7 1,423,471 + pheA SO1367 0.26 +0.1 1,423,479 - pheA SO1367 0.26 -2.3 1,423,655 + pheA SO1367 0.35 -2.7 1,423,663 - pheA SO1367 0.35 -1.2 1,423,718 - pheA SO1367 0.38 -0.9 1,423,741 + pheA SO1367 0.39 +0.1 1,423,741 + pheA SO1367 0.39 -1.2 1,423,816 + pheA SO1367 0.43 -0.5 1,423,816 + pheA SO1367 0.43 -0.1 1,423,824 - pheA SO1367 0.43 -0.3 1,423,824 - pheA SO1367 0.43 -0.1 1,423,855 - pheA SO1367 0.45 -4.9
Or see this region's nucleotide sequence