Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0968

Experiment: Def Chloride 400 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0967 and Psest_0968 are separated by 36 nucleotidesPsest_0968 and Psest_0969 overlap by 4 nucleotidesPsest_0969 and Psest_0970 overlap by 23 nucleotidesPsest_0970 and Psest_0971 are separated by 35 nucleotides Psest_0967: Psest_0967 - carbamoyl-phosphate synthase, small subunit, at 1,048,621 to 1,049,769 _0967 Psest_0968: Psest_0968 - carbamoyl-phosphate synthase, large subunit, at 1,049,806 to 1,053,027 _0968 Psest_0969: Psest_0969 - transcription elongation factor GreA, at 1,053,024 to 1,053,500 _0969 Psest_0970: Psest_0970 - hypothetical protein, at 1,053,478 to 1,053,909 _0970 Psest_0971: Psest_0971 - Predicted RNA-binding protein containing KH domain, possibly ribosomal protein, at 1,053,945 to 1,054,256 _0971 Position (kb) 1049 1050 1051 1052 1053 1054Strain fitness (log2 ratio) -2 -1 0 1 2at 1053.302 kb on + strand, within Psest_0969at 1053.336 kb on + strand, within Psest_0969at 1053.336 kb on + strand, within Psest_0969at 1053.336 kb on + strand, within Psest_0969at 1053.336 kb on + strand, within Psest_0969at 1053.494 kb on + strandat 1053.495 kb on - strandat 1053.495 kb on - strandat 1053.844 kb on + strand, within Psest_0970at 1053.844 kb on + strand, within Psest_0970at 1053.844 kb on + strand, within Psest_0970at 1053.844 kb on + strand, within Psest_0970at 1053.845 kb on - strand, within Psest_0970

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Per-strain Table

Position Strand Gene LocusTag Fraction Def Chloride 400 mM
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1,053,302 + Psest_0969 0.58 -0.9
1,053,336 + Psest_0969 0.65 -1.9
1,053,336 + Psest_0969 0.65 +1.9
1,053,336 + Psest_0969 0.65 +1.1
1,053,336 + Psest_0969 0.65 -1.3
1,053,494 + +1.2
1,053,495 - -0.2
1,053,495 - -0.7
1,053,844 + Psest_0970 0.85 -0.2
1,053,844 + Psest_0970 0.85 -1.7
1,053,844 + Psest_0970 0.85 +0.8
1,053,844 + Psest_0970 0.85 +1.1
1,053,845 - Psest_0970 0.85 -0.8

Or see this region's nucleotide sequence