Experiment: Def Chloride 400 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0687 and Psest_0688 are separated by 89 nucleotides Psest_0688 and Psest_0689 are separated by 157 nucleotides Psest_0689 and Psest_0690 are separated by 116 nucleotides Psest_0690 and Psest_0691 are separated by 204 nucleotides
Psest_0687: Psest_0687 - ATP phosphoribosyltransferase, regulatory subunit, at 750,741 to 751,928
_0687
Psest_0688: Psest_0688 - adenylosuccinate synthase, at 752,018 to 753,313
_0688
Psest_0689: Psest_0689 - tRNA_Leu_CAG, at 753,471 to 753,557
_0689
Psest_0690: Psest_0690 - tRNA_Leu_CAG, at 753,674 to 753,760
_0690
Psest_0691: Psest_0691 - ribonuclease R, at 753,965 to 756,466
_0691
Position (kb)
752
753
754 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 751.022 kb on + strand, within Psest_0687 at 751.022 kb on + strand, within Psest_0687 at 751.022 kb on + strand, within Psest_0687 at 751.022 kb on + strand, within Psest_0687 at 751.022 kb on + strand, within Psest_0687 at 751.022 kb on + strand, within Psest_0687 at 751.373 kb on + strand, within Psest_0687 at 751.373 kb on + strand, within Psest_0687 at 751.373 kb on + strand, within Psest_0687 at 751.373 kb on + strand, within Psest_0687 at 751.373 kb on + strand, within Psest_0687 at 751.374 kb on - strand, within Psest_0687 at 751.374 kb on - strand, within Psest_0687 at 751.374 kb on - strand, within Psest_0687 at 751.374 kb on - strand, within Psest_0687 at 751.374 kb on - strand, within Psest_0687 at 751.374 kb on - strand, within Psest_0687 at 751.374 kb on - strand, within Psest_0687 at 751.374 kb on - strand, within Psest_0687 at 751.378 kb on + strand, within Psest_0687 at 751.378 kb on + strand, within Psest_0687 at 751.378 kb on + strand, within Psest_0687 at 751.378 kb on + strand, within Psest_0687 at 751.378 kb on + strand, within Psest_0687 at 751.378 kb on + strand, within Psest_0687 at 751.378 kb on + strand, within Psest_0687 at 751.530 kb on + strand, within Psest_0687 at 751.530 kb on + strand, within Psest_0687 at 751.530 kb on + strand, within Psest_0687 at 751.557 kb on + strand, within Psest_0687 at 751.568 kb on + strand, within Psest_0687 at 751.568 kb on + strand, within Psest_0687 at 751.588 kb on + strand, within Psest_0687 at 751.588 kb on + strand, within Psest_0687 at 751.632 kb on + strand, within Psest_0687 at 751.892 kb on + strand at 753.305 kb on + strand at 753.306 kb on - strand at 753.529 kb on - strand, within Psest_0689 at 753.596 kb on - strand at 753.671 kb on - strand at 753.871 kb on - strand at 753.939 kb on - strand at 753.964 kb on - strand at 754.017 kb on - strand at 754.185 kb on - strand at 754.185 kb on - strand at 754.185 kb on - strand at 754.202 kb on + strand at 754.202 kb on + strand at 754.202 kb on + strand at 754.203 kb on - strand at 754.203 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Chloride 400 mM remove 751,022 + Psest_0687 0.24 -2.1 751,022 + Psest_0687 0.24 -1.3 751,022 + Psest_0687 0.24 -2.6 751,022 + Psest_0687 0.24 -1.6 751,022 + Psest_0687 0.24 -4.2 751,022 + Psest_0687 0.24 -4.0 751,373 + Psest_0687 0.53 -2.5 751,373 + Psest_0687 0.53 -2.7 751,373 + Psest_0687 0.53 -2.7 751,373 + Psest_0687 0.53 +0.4 751,373 + Psest_0687 0.53 -3.2 751,374 - Psest_0687 0.53 -2.6 751,374 - Psest_0687 0.53 -1.9 751,374 - Psest_0687 0.53 -1.6 751,374 - Psest_0687 0.53 -1.7 751,374 - Psest_0687 0.53 -2.6 751,374 - Psest_0687 0.53 -0.9 751,374 - Psest_0687 0.53 -1.8 751,374 - Psest_0687 0.53 -2.4 751,378 + Psest_0687 0.54 -2.2 751,378 + Psest_0687 0.54 -3.6 751,378 + Psest_0687 0.54 -3.6 751,378 + Psest_0687 0.54 -4.4 751,378 + Psest_0687 0.54 -2.6 751,378 + Psest_0687 0.54 -2.1 751,378 + Psest_0687 0.54 -2.8 751,530 + Psest_0687 0.66 -1.3 751,530 + Psest_0687 0.66 -2.6 751,530 + Psest_0687 0.66 -1.9 751,557 + Psest_0687 0.69 -1.8 751,568 + Psest_0687 0.70 -2.0 751,568 + Psest_0687 0.70 -1.8 751,588 + Psest_0687 0.71 -4.2 751,588 + Psest_0687 0.71 -3.3 751,632 + Psest_0687 0.75 -0.9 751,892 + -3.3 753,305 + +0.2 753,306 - -0.6 753,529 - Psest_0689 0.67 -1.2 753,596 - +1.0 753,671 - +0.1 753,871 - -1.3 753,939 - -3.0 753,964 - -1.8 754,017 - -1.0 754,185 - -1.1 754,185 - -0.9 754,185 - +0.3 754,202 + -0.6 754,202 + -0.8 754,202 + -0.2 754,203 - -2.7 754,203 - +0.3
Or see this region's nucleotide sequence