Experiment: Def Chloride 400 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3675 and Psest_3676 are separated by 6 nucleotides Psest_3676 and Psest_3677 overlap by 10 nucleotides Psest_3677 and Psest_3678 are separated by 75 nucleotides Psest_3678 and Psest_3679 are separated by 14 nucleotides
Psest_3675: Psest_3675 - tRNA nucleotidyltransferase/poly(A) polymerase, at 3,840,178 to 3,841,347
_3675
Psest_3676: Psest_3676 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase, at 3,841,354 to 3,841,896
_3676
Psest_3677: Psest_3677 - dihydroneopterin aldolase, at 3,841,887 to 3,842,240
_3677
Psest_3678: Psest_3678 - acyl-phosphate glycerol 3-phosphate acyltransferase, at 3,842,316 to 3,842,885
_3678
Psest_3679: Psest_3679 - putative glycoprotease GCP, at 3,842,900 to 3,843,925
_3679
Position (kb)
3842
3843 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3841.325 kb on - strand at 3841.325 kb on - strand at 3841.325 kb on - strand at 3841.345 kb on + strand at 3841.345 kb on + strand at 3841.345 kb on + strand at 3841.346 kb on - strand at 3841.346 kb on - strand at 3841.346 kb on - strand at 3841.346 kb on - strand at 3841.346 kb on - strand at 3841.465 kb on + strand, within Psest_3676 at 3842.441 kb on + strand, within Psest_3678 at 3842.441 kb on + strand, within Psest_3678 at 3842.441 kb on + strand, within Psest_3678 at 3842.441 kb on + strand, within Psest_3678 at 3842.441 kb on + strand, within Psest_3678 at 3842.441 kb on + strand, within Psest_3678 at 3842.516 kb on - strand, within Psest_3678 at 3842.601 kb on + strand, within Psest_3678 at 3842.602 kb on - strand, within Psest_3678 at 3842.822 kb on + strand, within Psest_3678 at 3842.822 kb on + strand, within Psest_3678 at 3842.823 kb on - strand, within Psest_3678 at 3842.823 kb on - strand, within Psest_3678 at 3842.823 kb on - strand, within Psest_3678
Per-strain Table
Position Strand Gene LocusTag Fraction Def Chloride 400 mM remove 3,841,325 - -0.2 3,841,325 - +2.4 3,841,325 - -1.0 3,841,345 + -0.1 3,841,345 + +1.4 3,841,345 + -0.8 3,841,346 - -1.6 3,841,346 - +2.0 3,841,346 - -1.7 3,841,346 - -0.6 3,841,346 - -1.1 3,841,465 + Psest_3676 0.20 +1.4 3,842,441 + Psest_3678 0.22 -0.1 3,842,441 + Psest_3678 0.22 +2.9 3,842,441 + Psest_3678 0.22 -1.3 3,842,441 + Psest_3678 0.22 +0.7 3,842,441 + Psest_3678 0.22 -0.7 3,842,441 + Psest_3678 0.22 -0.3 3,842,516 - Psest_3678 0.35 -0.9 3,842,601 + Psest_3678 0.50 +0.4 3,842,602 - Psest_3678 0.50 -1.4 3,842,822 + Psest_3678 0.89 -2.1 3,842,822 + Psest_3678 0.89 +0.9 3,842,823 - Psest_3678 0.89 +0.0 3,842,823 - Psest_3678 0.89 -1.9 3,842,823 - Psest_3678 0.89 -2.8
Or see this region's nucleotide sequence