Experiment: Def Chloride 400 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0290 and Psest_0291 overlap by 1 nucleotides Psest_0291 and Psest_0292 are separated by 57 nucleotides Psest_0292 and Psest_0293 are separated by 22 nucleotides
Psest_0290: Psest_0290 - dihydroorotase, multifunctional complex type, at 305,220 to 306,494
_0290
Psest_0291: Psest_0291 - aspartate carbamoyltransferase, at 306,494 to 307,510
_0291
Psest_0292: Psest_0292 - Pyrimidine operon attenuation protein/uracil phosphoribosyltransferase, at 307,568 to 308,080
_0292
Psest_0293: Psest_0293 - RNAse H-fold protein YqgF, at 308,103 to 308,531
_0293
Position (kb) 306
307
308 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 305.659 kb on - strand, within Psest_0290 at 305.987 kb on - strand, within Psest_0290 at 306.595 kb on - strand at 306.595 kb on - strand at 306.595 kb on - strand at 306.732 kb on + strand, within Psest_0291 at 306.732 kb on + strand, within Psest_0291 at 306.741 kb on + strand, within Psest_0291 at 307.482 kb on - strand at 307.545 kb on + strand at 307.545 kb on + strand at 307.557 kb on + strand at 307.667 kb on + strand, within Psest_0292 at 307.667 kb on + strand, within Psest_0292 at 307.667 kb on + strand, within Psest_0292 at 307.668 kb on - strand, within Psest_0292 at 307.668 kb on - strand, within Psest_0292 at 307.668 kb on - strand, within Psest_0292 at 308.022 kb on + strand, within Psest_0292
Per-strain Table
Position Strand Gene LocusTag Fraction Def Chloride 400 mM remove 305,659 - Psest_0290 0.34 -0.8 305,987 - Psest_0290 0.60 -0.8 306,595 - -1.1 306,595 - -0.8 306,595 - -0.1 306,732 + Psest_0291 0.23 -1.2 306,732 + Psest_0291 0.23 -1.3 306,741 + Psest_0291 0.24 +0.5 307,482 - -0.7 307,545 + -1.5 307,545 + -0.1 307,557 + -1.7 307,667 + Psest_0292 0.19 -2.3 307,667 + Psest_0292 0.19 -3.4 307,667 + Psest_0292 0.19 -2.2 307,668 - Psest_0292 0.19 -0.6 307,668 - Psest_0292 0.19 +0.0 307,668 - Psest_0292 0.19 -0.8 308,022 + Psest_0292 0.88 -0.9
Or see this region's nucleotide sequence