Experiment: Def Chloride 400 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0115 and Psest_0116 are separated by 16 nucleotides Psest_0116 and Psest_0117 overlap by 4 nucleotides Psest_0117 and Psest_0118 overlap by 4 nucleotides Psest_0118 and Psest_0119 are separated by 21 nucleotides Psest_0119 and Psest_0120 are separated by 213 nucleotides
Psest_0115: Psest_0115 - Predicted integral membrane protein, at 113,172 to 113,444
_0115
Psest_0116: Psest_0116 - Uncharacterized protein conserved in bacteria, at 113,461 to 114,288
_0116
Psest_0117: Psest_0117 - Uncharacterized protein conserved in bacteria (DUF2063)., at 114,285 to 115,076
_0117
Psest_0118: Psest_0118 - Predicted membrane protein, at 115,073 to 115,534
_0118
Psest_0119: Psest_0119 - hypothetical protein, at 115,556 to 115,762
_0119
Psest_0120: Psest_0120 - hypothetical protein, at 115,976 to 116,320
_0120
Position (kb)
114
115
116 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 113.384 kb on + strand, within Psest_0115 at 113.448 kb on + strand at 113.449 kb on - strand at 113.484 kb on - strand at 113.563 kb on + strand, within Psest_0116 at 113.563 kb on + strand, within Psest_0116 at 113.563 kb on + strand, within Psest_0116 at 113.563 kb on + strand, within Psest_0116 at 113.563 kb on + strand, within Psest_0116 at 113.563 kb on + strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.767 kb on + strand, within Psest_0116 at 113.767 kb on + strand, within Psest_0116 at 113.767 kb on + strand, within Psest_0116 at 113.767 kb on + strand, within Psest_0116 at 113.767 kb on + strand, within Psest_0116 at 113.767 kb on + strand, within Psest_0116 at 113.768 kb on - strand, within Psest_0116 at 113.768 kb on - strand, within Psest_0116 at 113.768 kb on - strand, within Psest_0116 at 113.768 kb on - strand, within Psest_0116 at 113.768 kb on - strand, within Psest_0116 at 113.768 kb on - strand, within Psest_0116 at 113.768 kb on - strand, within Psest_0116 at 113.888 kb on - strand, within Psest_0116 at 113.888 kb on - strand, within Psest_0116 at 114.505 kb on - strand, within Psest_0117 at 114.505 kb on - strand, within Psest_0117 at 114.505 kb on - strand, within Psest_0117 at 114.547 kb on - strand, within Psest_0117 at 114.603 kb on + strand, within Psest_0117 at 114.604 kb on - strand, within Psest_0117 at 114.604 kb on - strand, within Psest_0117 at 114.777 kb on + strand, within Psest_0117 at 114.778 kb on - strand, within Psest_0117 at 114.778 kb on - strand, within Psest_0117 at 114.778 kb on - strand, within Psest_0117 at 114.778 kb on - strand, within Psest_0117 at 114.778 kb on - strand, within Psest_0117 at 114.907 kb on + strand, within Psest_0117 at 114.907 kb on + strand, within Psest_0117 at 114.907 kb on + strand, within Psest_0117 at 114.907 kb on + strand, within Psest_0117 at 114.907 kb on + strand, within Psest_0117 at 114.908 kb on - strand, within Psest_0117 at 114.908 kb on - strand, within Psest_0117 at 114.908 kb on - strand, within Psest_0117 at 114.908 kb on - strand, within Psest_0117 at 114.908 kb on - strand, within Psest_0117 at 114.908 kb on - strand, within Psest_0117 at 115.325 kb on + strand, within Psest_0118 at 115.325 kb on + strand, within Psest_0118 at 115.325 kb on + strand, within Psest_0118 at 115.325 kb on + strand, within Psest_0118 at 115.325 kb on + strand, within Psest_0118 at 115.326 kb on - strand, within Psest_0118 at 115.326 kb on - strand, within Psest_0118 at 115.326 kb on - strand, within Psest_0118 at 115.326 kb on - strand, within Psest_0118 at 115.326 kb on - strand, within Psest_0118 at 115.326 kb on - strand, within Psest_0118 at 115.326 kb on - strand, within Psest_0118 at 115.326 kb on - strand, within Psest_0118 at 115.473 kb on + strand, within Psest_0118 at 115.474 kb on - strand, within Psest_0118 at 115.474 kb on - strand, within Psest_0118 at 115.474 kb on - strand, within Psest_0118 at 115.650 kb on + strand, within Psest_0119 at 115.650 kb on + strand, within Psest_0119 at 115.651 kb on - strand, within Psest_0119 at 115.651 kb on - strand, within Psest_0119 at 115.651 kb on - strand, within Psest_0119 at 115.834 kb on - strand at 115.907 kb on - strand at 115.907 kb on - strand at 115.907 kb on - strand at 115.907 kb on - strand at 115.907 kb on - strand at 115.907 kb on - strand at 115.907 kb on - strand at 115.907 kb on - strand at 115.933 kb on - strand at 115.933 kb on - strand at 115.933 kb on - strand at 115.933 kb on - strand at 115.933 kb on - strand at 115.985 kb on - strand at 115.985 kb on - strand at 115.988 kb on - strand at 115.991 kb on + strand at 116.026 kb on + strand, within Psest_0120 at 116.065 kb on - strand, within Psest_0120
Per-strain Table
Position Strand Gene LocusTag Fraction Def Chloride 400 mM remove 113,384 + Psest_0115 0.78 -1.1 113,448 + -1.3 113,449 - +1.3 113,484 - -0.5 113,563 + Psest_0116 0.12 -0.5 113,563 + Psest_0116 0.12 -0.9 113,563 + Psest_0116 0.12 -0.1 113,563 + Psest_0116 0.12 +0.5 113,563 + Psest_0116 0.12 +0.7 113,563 + Psest_0116 0.12 +0.1 113,564 - Psest_0116 0.12 +0.2 113,564 - Psest_0116 0.12 +0.5 113,564 - Psest_0116 0.12 +0.9 113,564 - Psest_0116 0.12 +0.5 113,564 - Psest_0116 0.12 -0.0 113,564 - Psest_0116 0.12 +1.3 113,564 - Psest_0116 0.12 +1.2 113,564 - Psest_0116 0.12 -0.9 113,767 + Psest_0116 0.37 -0.3 113,767 + Psest_0116 0.37 -2.1 113,767 + Psest_0116 0.37 +1.5 113,767 + Psest_0116 0.37 +2.5 113,767 + Psest_0116 0.37 -0.6 113,767 + Psest_0116 0.37 +1.2 113,768 - Psest_0116 0.37 +3.5 113,768 - Psest_0116 0.37 +2.5 113,768 - Psest_0116 0.37 -1.2 113,768 - Psest_0116 0.37 -0.7 113,768 - Psest_0116 0.37 +2.3 113,768 - Psest_0116 0.37 -0.9 113,768 - Psest_0116 0.37 +1.2 113,888 - Psest_0116 0.52 -0.5 113,888 - Psest_0116 0.52 +0.9 114,505 - Psest_0117 0.28 -0.4 114,505 - Psest_0117 0.28 +0.7 114,505 - Psest_0117 0.28 +1.2 114,547 - Psest_0117 0.33 -2.8 114,603 + Psest_0117 0.40 -0.1 114,604 - Psest_0117 0.40 +0.3 114,604 - Psest_0117 0.40 -2.1 114,777 + Psest_0117 0.62 -0.9 114,778 - Psest_0117 0.62 +0.4 114,778 - Psest_0117 0.62 -1.2 114,778 - Psest_0117 0.62 +1.5 114,778 - Psest_0117 0.62 -0.5 114,778 - Psest_0117 0.62 -1.2 114,907 + Psest_0117 0.79 +0.2 114,907 + Psest_0117 0.79 +0.1 114,907 + Psest_0117 0.79 -0.9 114,907 + Psest_0117 0.79 -1.3 114,907 + Psest_0117 0.79 +0.8 114,908 - Psest_0117 0.79 -0.6 114,908 - Psest_0117 0.79 -1.0 114,908 - Psest_0117 0.79 -0.1 114,908 - Psest_0117 0.79 +0.9 114,908 - Psest_0117 0.79 -2.5 114,908 - Psest_0117 0.79 -0.6 115,325 + Psest_0118 0.55 +0.1 115,325 + Psest_0118 0.55 +1.6 115,325 + Psest_0118 0.55 +0.6 115,325 + Psest_0118 0.55 -0.9 115,325 + Psest_0118 0.55 +1.6 115,326 - Psest_0118 0.55 +0.9 115,326 - Psest_0118 0.55 +0.5 115,326 - Psest_0118 0.55 +0.5 115,326 - Psest_0118 0.55 -0.1 115,326 - Psest_0118 0.55 -1.6 115,326 - Psest_0118 0.55 +0.8 115,326 - Psest_0118 0.55 +1.5 115,326 - Psest_0118 0.55 -1.7 115,473 + Psest_0118 0.87 -0.6 115,474 - Psest_0118 0.87 -0.8 115,474 - Psest_0118 0.87 -0.5 115,474 - Psest_0118 0.87 +0.1 115,650 + Psest_0119 0.45 +0.8 115,650 + Psest_0119 0.45 +1.5 115,651 - Psest_0119 0.46 +1.1 115,651 - Psest_0119 0.46 -1.2 115,651 - Psest_0119 0.46 -0.1 115,834 - +0.3 115,907 - -1.3 115,907 - -2.2 115,907 - +0.2 115,907 - +1.1 115,907 - -1.1 115,907 - -1.1 115,907 - -0.3 115,907 - -2.2 115,933 - -0.1 115,933 - +0.7 115,933 - +1.1 115,933 - -0.3 115,933 - +0.1 115,985 - -2.0 115,985 - -1.0 115,988 - +1.2 115,991 + -1.0 116,026 + Psest_0120 0.14 -1.9 116,065 - Psest_0120 0.26 -2.5
Or see this region's nucleotide sequence