Strain Fitness in Shewanella oneidensis MR-1 around SO3415

Experiment: Glycine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthrB and thrA overlap by 4 nucleotidesthrA and slyD are separated by 641 nucleotides SO3414: thrB - homoserine kinase (NCBI ptt file), at 3,554,272 to 3,555,210 thrB SO3415: thrA - aspartokinase I/homoserine dehydrogenase, threonine-sensitive (NCBI ptt file), at 3,555,207 to 3,557,675 thrA SO3417: slyD - peptidyl-prolyl cis-trans isomerase SlyD (NCBI ptt file), at 3,558,317 to 3,558,985 slyD Position (kb) 3555 3556 3557 3558Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 3554.221 kb on - strandat 3554.224 kb on + strandat 3554.232 kb on - strandat 3554.244 kb on + strandat 3554.279 kb on + strandat 3554.279 kb on + strandat 3554.279 kb on + strandat 3554.481 kb on - strand, within thrBat 3554.498 kb on + strand, within thrBat 3554.505 kb on - strand, within thrBat 3554.556 kb on + strand, within thrBat 3554.612 kb on - strand, within thrBat 3554.612 kb on - strand, within thrBat 3554.656 kb on + strand, within thrBat 3554.689 kb on - strand, within thrBat 3554.689 kb on - strand, within thrBat 3554.694 kb on + strand, within thrBat 3554.694 kb on + strand, within thrBat 3554.784 kb on + strand, within thrBat 3554.795 kb on - strand, within thrBat 3554.892 kb on + strand, within thrBat 3554.911 kb on + strand, within thrBat 3554.918 kb on - strand, within thrBat 3554.955 kb on - strand, within thrBat 3555.075 kb on + strand, within thrBat 3555.092 kb on + strand, within thrBat 3555.206 kb on + strandat 3555.206 kb on + strandat 3555.218 kb on + strandat 3555.224 kb on - strandat 3555.280 kb on - strandat 3555.295 kb on - strandat 3555.305 kb on - strandat 3555.310 kb on - strandat 3555.398 kb on - strandat 3555.439 kb on - strandat 3555.448 kb on + strandat 3555.448 kb on + strandat 3555.456 kb on - strand, within thrAat 3555.456 kb on - strand, within thrAat 3555.638 kb on + strand, within thrAat 3555.726 kb on - strand, within thrAat 3555.780 kb on - strand, within thrAat 3555.878 kb on + strand, within thrAat 3556.011 kb on - strand, within thrAat 3556.027 kb on + strand, within thrAat 3556.058 kb on + strand, within thrAat 3556.230 kb on + strand, within thrAat 3556.230 kb on + strand, within thrAat 3556.234 kb on - strand, within thrAat 3556.271 kb on + strand, within thrAat 3556.299 kb on + strand, within thrAat 3556.330 kb on + strand, within thrAat 3556.330 kb on + strand, within thrAat 3556.330 kb on + strand, within thrAat 3556.330 kb on + strand, within thrAat 3556.330 kb on + strand, within thrAat 3556.338 kb on - strand, within thrAat 3556.338 kb on - strand, within thrAat 3556.338 kb on - strand, within thrAat 3556.364 kb on + strand, within thrAat 3556.364 kb on + strand, within thrAat 3556.418 kb on - strand, within thrAat 3556.442 kb on + strand, within thrAat 3556.512 kb on + strand, within thrAat 3556.859 kb on + strand, within thrAat 3556.875 kb on - strand, within thrAat 3556.875 kb on - strand, within thrAat 3557.046 kb on + strand, within thrAat 3557.064 kb on - strand, within thrAat 3557.064 kb on - strand, within thrAat 3557.070 kb on + strand, within thrAat 3557.070 kb on + strand, within thrAat 3557.176 kb on - strand, within thrAat 3557.233 kb on - strand, within thrAat 3557.256 kb on - strand, within thrAat 3557.424 kb on + strand, within thrAat 3557.437 kb on - strandat 3557.547 kb on - strandat 3557.573 kb on - strandat 3557.682 kb on + strandat 3557.690 kb on - strandat 3557.711 kb on - strandat 3557.721 kb on - strandat 3557.766 kb on - strandat 3557.779 kb on - strandat 3557.842 kb on - strandat 3557.851 kb on + strandat 3557.859 kb on - strandat 3557.859 kb on - strandat 3558.135 kb on - strandat 3558.227 kb on - strandat 3558.306 kb on - strandat 3558.549 kb on - strand, within slyD

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Per-strain Table

Position Strand Gene LocusTag Fraction Glycine (N)
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3,554,221 - -0.7
3,554,224 + -3.3
3,554,232 - -0.7
3,554,244 + -2.9
3,554,279 + -4.3
3,554,279 + -2.6
3,554,279 + -2.0
3,554,481 - thrB SO3414 0.22 -2.5
3,554,498 + thrB SO3414 0.24 -2.5
3,554,505 - thrB SO3414 0.25 -3.4
3,554,556 + thrB SO3414 0.30 -1.9
3,554,612 - thrB SO3414 0.36 -1.9
3,554,612 - thrB SO3414 0.36 -4.6
3,554,656 + thrB SO3414 0.41 -3.5
3,554,689 - thrB SO3414 0.44 -4.6
3,554,689 - thrB SO3414 0.44 -2.4
3,554,694 + thrB SO3414 0.45 -2.1
3,554,694 + thrB SO3414 0.45 -4.9
3,554,784 + thrB SO3414 0.55 +0.3
3,554,795 - thrB SO3414 0.56 -2.2
3,554,892 + thrB SO3414 0.66 -4.4
3,554,911 + thrB SO3414 0.68 -3.1
3,554,918 - thrB SO3414 0.69 -2.2
3,554,955 - thrB SO3414 0.73 -3.6
3,555,075 + thrB SO3414 0.86 -3.0
3,555,092 + thrB SO3414 0.87 -3.6
3,555,206 + -3.2
3,555,206 + -2.5
3,555,218 + -1.6
3,555,224 - -2.9
3,555,280 - -2.2
3,555,295 - -1.0
3,555,305 - -1.9
3,555,310 - -2.9
3,555,398 - -2.8
3,555,439 - -3.1
3,555,448 + -2.5
3,555,448 + -2.2
3,555,456 - thrA SO3415 0.10 -3.2
3,555,456 - thrA SO3415 0.10 -3.9
3,555,638 + thrA SO3415 0.17 -3.7
3,555,726 - thrA SO3415 0.21 -4.4
3,555,780 - thrA SO3415 0.23 -2.6
3,555,878 + thrA SO3415 0.27 -4.6
3,556,011 - thrA SO3415 0.33 -1.4
3,556,027 + thrA SO3415 0.33 -2.8
3,556,058 + thrA SO3415 0.34 -4.6
3,556,230 + thrA SO3415 0.41 -2.9
3,556,230 + thrA SO3415 0.41 -3.9
3,556,234 - thrA SO3415 0.42 -4.8
3,556,271 + thrA SO3415 0.43 -2.2
3,556,299 + thrA SO3415 0.44 -4.3
3,556,330 + thrA SO3415 0.45 -2.9
3,556,330 + thrA SO3415 0.45 +0.0
3,556,330 + thrA SO3415 0.45 -3.1
3,556,330 + thrA SO3415 0.45 -2.6
3,556,330 + thrA SO3415 0.45 -2.4
3,556,338 - thrA SO3415 0.46 -3.5
3,556,338 - thrA SO3415 0.46 -4.6
3,556,338 - thrA SO3415 0.46 -2.6
3,556,364 + thrA SO3415 0.47 -3.6
3,556,364 + thrA SO3415 0.47 -4.1
3,556,418 - thrA SO3415 0.49 -3.1
3,556,442 + thrA SO3415 0.50 -2.8
3,556,512 + thrA SO3415 0.53 -2.7
3,556,859 + thrA SO3415 0.67 -1.1
3,556,875 - thrA SO3415 0.68 -3.3
3,556,875 - thrA SO3415 0.68 -3.8
3,557,046 + thrA SO3415 0.74 -1.3
3,557,064 - thrA SO3415 0.75 -3.2
3,557,064 - thrA SO3415 0.75 -2.0
3,557,070 + thrA SO3415 0.75 -4.0
3,557,070 + thrA SO3415 0.75 -3.3
3,557,176 - thrA SO3415 0.80 -2.2
3,557,233 - thrA SO3415 0.82 -3.6
3,557,256 - thrA SO3415 0.83 -4.0
3,557,424 + thrA SO3415 0.90 -4.4
3,557,437 - -2.8
3,557,547 - -1.5
3,557,573 - -4.3
3,557,682 + -2.2
3,557,690 - +0.7
3,557,711 - -0.4
3,557,721 - +0.1
3,557,766 - -4.0
3,557,779 - -3.3
3,557,842 - +0.4
3,557,851 + -2.0
3,557,859 - -2.9
3,557,859 - -1.4
3,558,135 - -0.0
3,558,227 - -0.3
3,558,306 - +1.4
3,558,549 - slyD SO3417 0.35 +0.8

Or see this region's nucleotide sequence