Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1651 and SO1652 are separated by 120 nucleotides SO1652 and rfbD are separated by 16 nucleotides rfbD and SO1654 overlap by 26 nucleotides SO1654 and cysQ-2 are separated by 15 nucleotides
SO1651: SO1651 - Snf2 family protein (NCBI ptt file), at 1,737,303 to 1,740,515
SO1651
SO1652: SO1652 - conserved hypothetical protein (NCBI ptt file), at 1,740,636 to 1,741,394
SO1652
SO1653: rfbD - dTDP-4-dehydrorhamnose reductase (NCBI ptt file), at 1,741,411 to 1,742,397
rfbD
SO1654: SO1654 - hypothetical protein (NCBI ptt file), at 1,742,372 to 1,742,725
SO1654
SO1655: cysQ-2 - cysQ protein (NCBI ptt file), at 1,742,741 to 1,743,502
cysQ-2
Position (kb)
1741
1742
1743 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1740.482 kb on - strand at 1740.482 kb on - strand at 1740.505 kb on - strand at 1740.517 kb on - strand at 1740.558 kb on + strand at 1740.686 kb on + strand at 1740.694 kb on - strand at 1740.773 kb on - strand, within SO1652 at 1740.914 kb on + strand, within SO1652 at 1740.967 kb on + strand, within SO1652 at 1740.972 kb on + strand, within SO1652 at 1740.989 kb on + strand, within SO1652 at 1740.989 kb on + strand, within SO1652 at 1740.989 kb on + strand, within SO1652 at 1741.120 kb on + strand, within SO1652 at 1741.190 kb on - strand, within SO1652 at 1741.295 kb on + strand, within SO1652 at 1741.316 kb on - strand, within SO1652 at 1742.391 kb on + strand at 1742.392 kb on + strand at 1742.399 kb on - strand at 1742.407 kb on + strand at 1742.531 kb on + strand, within SO1654 at 1742.538 kb on - strand, within SO1654 at 1742.566 kb on + strand, within SO1654 at 1742.566 kb on + strand, within SO1654 at 1742.566 kb on + strand, within SO1654 at 1742.566 kb on + strand, within SO1654 at 1742.566 kb on + strand, within SO1654 at 1743.129 kb on - strand, within cysQ-2 at 1743.338 kb on - strand, within cysQ-2
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 1,740,482 - +0.6 1,740,482 - -0.7 1,740,505 - -0.3 1,740,517 - +0.7 1,740,558 + +0.7 1,740,686 + +0.2 1,740,694 - -0.3 1,740,773 - SO1652 0.18 +0.2 1,740,914 + SO1652 0.37 -0.3 1,740,967 + SO1652 0.44 +0.5 1,740,972 + SO1652 0.44 +0.2 1,740,989 + SO1652 0.47 +0.1 1,740,989 + SO1652 0.47 -0.5 1,740,989 + SO1652 0.47 -0.5 1,741,120 + SO1652 0.64 +0.5 1,741,190 - SO1652 0.73 -0.6 1,741,295 + SO1652 0.87 -0.1 1,741,316 - SO1652 0.90 -1.0 1,742,391 + +0.7 1,742,392 + -0.1 1,742,399 - +0.6 1,742,407 + -0.8 1,742,531 + SO1654 0.45 -0.3 1,742,538 - SO1654 0.47 +0.2 1,742,566 + SO1654 0.55 +1.0 1,742,566 + SO1654 0.55 +0.2 1,742,566 + SO1654 0.55 -0.3 1,742,566 + SO1654 0.55 -0.3 1,742,566 + SO1654 0.55 +0.3 1,743,129 - cysQ-2 SO1655 0.51 -5.2 1,743,338 - cysQ-2 SO1655 0.78 -3.7
Or see this region's nucleotide sequence