Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1290 and folB are separated by 261 nucleotides folB and folK-2 are separated by 42 nucleotides folK-2 and SO1293 are separated by 18 nucleotides SO1293 and SO1294 are separated by 84 nucleotides
SO1290: SO1290 - conserved hypothetical protein TIGR00023 (NCBI ptt file), at 1,341,340 to 1,341,951
SO1290
SO1291: folB - dihydroneopterin aldolase (NCBI ptt file), at 1,342,213 to 1,342,563
folB
SO1292: folK-2 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (NCBI ptt file), at 1,342,606 to 1,343,109
folK-2
SO1293: SO1293 - undecaprenol kinase, putative (NCBI ptt file), at 1,343,128 to 1,343,928
SO1293
SO1294: SO1294 - ribosomal RNA large subunit methyltransferase A, putative (NCBI ptt file), at 1,344,013 to 1,344,840
SO1294
Position (kb)
1342
1343
1344 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1341.624 kb on + strand, within SO1290 at 1341.688 kb on + strand, within SO1290 at 1341.693 kb on + strand, within SO1290 at 1341.725 kb on - strand, within SO1290 at 1341.749 kb on + strand, within SO1290 at 1341.757 kb on - strand, within SO1290 at 1341.805 kb on - strand, within SO1290 at 1341.930 kb on - strand at 1341.968 kb on - strand at 1342.019 kb on + strand at 1342.019 kb on + strand at 1342.019 kb on + strand at 1342.027 kb on - strand at 1342.027 kb on - strand at 1342.152 kb on + strand at 1342.158 kb on + strand at 1342.176 kb on + strand at 1342.592 kb on + strand at 1342.592 kb on + strand at 1342.599 kb on + strand at 1342.599 kb on - strand at 1342.624 kb on + strand at 1342.645 kb on - strand at 1342.927 kb on + strand, within folK-2 at 1342.971 kb on - strand, within folK-2 at 1343.013 kb on + strand, within folK-2 at 1343.090 kb on + strand at 1343.161 kb on + strand at 1343.223 kb on + strand, within SO1293 at 1343.223 kb on + strand, within SO1293 at 1343.231 kb on - strand, within SO1293 at 1343.231 kb on - strand, within SO1293 at 1343.247 kb on - strand, within SO1293 at 1343.253 kb on - strand, within SO1293 at 1343.268 kb on + strand, within SO1293 at 1343.313 kb on + strand, within SO1293 at 1343.321 kb on - strand, within SO1293 at 1343.321 kb on - strand, within SO1293 at 1343.321 kb on - strand, within SO1293 at 1343.332 kb on - strand, within SO1293 at 1343.356 kb on + strand, within SO1293 at 1343.357 kb on - strand, within SO1293 at 1343.642 kb on + strand, within SO1293 at 1343.797 kb on + strand, within SO1293 at 1343.905 kb on - strand at 1343.910 kb on - strand at 1343.915 kb on - strand at 1344.017 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 1,341,624 + SO1290 0.46 +2.5 1,341,688 + SO1290 0.57 +1.7 1,341,693 + SO1290 0.58 +0.8 1,341,725 - SO1290 0.63 +1.0 1,341,749 + SO1290 0.67 +1.3 1,341,757 - SO1290 0.68 +0.9 1,341,805 - SO1290 0.76 +1.1 1,341,930 - +1.4 1,341,968 - -0.5 1,342,019 + +1.0 1,342,019 + +1.6 1,342,019 + +1.4 1,342,027 - -2.6 1,342,027 - -1.3 1,342,152 + +0.5 1,342,158 + +0.8 1,342,176 + +0.4 1,342,592 + -0.7 1,342,592 + -0.9 1,342,599 + -0.7 1,342,599 - -3.1 1,342,624 + -0.6 1,342,645 - -2.5 1,342,927 + folK-2 SO1292 0.64 -2.7 1,342,971 - folK-2 SO1292 0.72 -3.0 1,343,013 + folK-2 SO1292 0.81 -1.6 1,343,090 + -1.6 1,343,161 + -0.1 1,343,223 + SO1293 0.12 -0.3 1,343,223 + SO1293 0.12 +0.3 1,343,231 - SO1293 0.13 +0.1 1,343,231 - SO1293 0.13 -0.2 1,343,247 - SO1293 0.15 -1.7 1,343,253 - SO1293 0.16 +1.3 1,343,268 + SO1293 0.17 +0.9 1,343,313 + SO1293 0.23 -0.4 1,343,321 - SO1293 0.24 +0.1 1,343,321 - SO1293 0.24 +0.6 1,343,321 - SO1293 0.24 +0.7 1,343,332 - SO1293 0.25 -0.1 1,343,356 + SO1293 0.28 +0.6 1,343,357 - SO1293 0.29 +0.8 1,343,642 + SO1293 0.64 -1.7 1,343,797 + SO1293 0.84 +0.5 1,343,905 - -0.8 1,343,910 - +1.4 1,343,915 - -0.2 1,344,017 - +0.5
Or see this region's nucleotide sequence