Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1034 and cobT are separated by 312 nucleotides cobT and cobS are separated by 106 nucleotides cobS and cobU overlap by 4 nucleotides
SO1034: SO1034 - iron-compound ABC transporter, permease protein (NCBI ptt file), at 1,072,962 to 1,074,104
SO1034
SO1035: cobT - nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (NCBI ptt file), at 1,074,417 to 1,075,469
cobT
SO1036: cobS - cobalamin 5'-phosphate synthase (NCBI ptt file), at 1,075,576 to 1,076,364
cobS
SO1037: cobU - cobinamide kinase/cobinamide phosphate guanylyltransferase (NCBI ptt file), at 1,076,361 to 1,076,966
cobU
Position (kb)
1074
1075
1076 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1073.463 kb on - strand, within SO1034 at 1073.544 kb on + strand, within SO1034 at 1073.784 kb on + strand, within SO1034 at 1073.784 kb on + strand, within SO1034 at 1073.784 kb on + strand, within SO1034 at 1073.791 kb on + strand, within SO1034 at 1073.792 kb on - strand, within SO1034 at 1073.792 kb on - strand, within SO1034 at 1073.792 kb on - strand, within SO1034 at 1073.792 kb on - strand, within SO1034 at 1073.792 kb on - strand, within SO1034 at 1073.799 kb on - strand, within SO1034 at 1073.834 kb on + strand, within SO1034 at 1073.900 kb on - strand, within SO1034 at 1073.905 kb on + strand, within SO1034 at 1073.963 kb on - strand, within SO1034 at 1074.016 kb on + strand at 1074.093 kb on - strand at 1074.121 kb on - strand at 1074.419 kb on - strand at 1074.424 kb on + strand at 1074.470 kb on - strand at 1074.474 kb on - strand at 1074.533 kb on + strand, within cobT at 1074.581 kb on - strand, within cobT at 1074.744 kb on - strand, within cobT at 1074.759 kb on + strand, within cobT at 1074.774 kb on - strand, within cobT at 1075.054 kb on + strand, within cobT at 1075.159 kb on + strand, within cobT at 1075.167 kb on - strand, within cobT at 1075.257 kb on - strand, within cobT at 1075.328 kb on + strand, within cobT at 1075.362 kb on + strand, within cobT at 1075.459 kb on - strand at 1075.461 kb on + strand at 1075.464 kb on + strand at 1075.469 kb on - strand at 1075.694 kb on - strand, within cobS at 1075.742 kb on - strand, within cobS at 1075.849 kb on + strand, within cobS at 1075.884 kb on - strand, within cobS at 1075.885 kb on + strand, within cobS at 1076.038 kb on + strand, within cobS at 1076.071 kb on - strand, within cobS at 1076.086 kb on - strand, within cobS at 1076.168 kb on + strand, within cobS at 1076.230 kb on + strand, within cobS at 1076.248 kb on - strand, within cobS at 1076.330 kb on + strand at 1076.373 kb on + strand at 1076.463 kb on - strand, within cobU
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 1,073,463 - SO1034 0.44 -1.8 1,073,544 + SO1034 0.51 -0.9 1,073,784 + SO1034 0.72 -1.0 1,073,784 + SO1034 0.72 -2.3 1,073,784 + SO1034 0.72 -0.4 1,073,791 + SO1034 0.73 -3.2 1,073,792 - SO1034 0.73 -1.9 1,073,792 - SO1034 0.73 -1.6 1,073,792 - SO1034 0.73 -2.4 1,073,792 - SO1034 0.73 -1.9 1,073,792 - SO1034 0.73 -1.7 1,073,799 - SO1034 0.73 -2.4 1,073,834 + SO1034 0.76 -1.9 1,073,900 - SO1034 0.82 -2.2 1,073,905 + SO1034 0.83 -4.0 1,073,963 - SO1034 0.88 -1.2 1,074,016 + -1.7 1,074,093 - -0.2 1,074,121 - +0.8 1,074,419 - -0.6 1,074,424 + -0.4 1,074,470 - -2.0 1,074,474 - -0.5 1,074,533 + cobT SO1035 0.11 -0.6 1,074,581 - cobT SO1035 0.16 -0.1 1,074,744 - cobT SO1035 0.31 -0.4 1,074,759 + cobT SO1035 0.32 +0.1 1,074,774 - cobT SO1035 0.34 +1.1 1,075,054 + cobT SO1035 0.60 +0.9 1,075,159 + cobT SO1035 0.70 -0.5 1,075,167 - cobT SO1035 0.71 -0.6 1,075,257 - cobT SO1035 0.80 +0.4 1,075,328 + cobT SO1035 0.87 +0.9 1,075,362 + cobT SO1035 0.90 -1.6 1,075,459 - -0.6 1,075,461 + -0.0 1,075,464 + +0.4 1,075,469 - -0.9 1,075,694 - cobS SO1036 0.15 -0.8 1,075,742 - cobS SO1036 0.21 -0.6 1,075,849 + cobS SO1036 0.35 -0.5 1,075,884 - cobS SO1036 0.39 -1.9 1,075,885 + cobS SO1036 0.39 -0.0 1,076,038 + cobS SO1036 0.59 +0.5 1,076,071 - cobS SO1036 0.63 +0.4 1,076,086 - cobS SO1036 0.65 -0.9 1,076,168 + cobS SO1036 0.75 +0.5 1,076,230 + cobS SO1036 0.83 +0.3 1,076,248 - cobS SO1036 0.85 -0.1 1,076,330 + -2.6 1,076,373 + -0.5 1,076,463 - cobU SO1037 0.17 -0.5
Or see this region's nucleotide sequence