Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_1021

Experiment: Def Chlorate 40 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_1019 and Psest_1020 are separated by 17 nucleotidesPsest_1020 and Psest_1021 are separated by 78 nucleotidesPsest_1021 and Psest_1022 are separated by 213 nucleotides Psest_1019: Psest_1019 - acetyl-CoA carboxylase, biotin carboxylase subunit, at 1,105,699 to 1,107,048 _1019 Psest_1020: Psest_1020 - acetyl-CoA carboxylase, biotin carboxyl carrier protein, at 1,107,066 to 1,107,521 _1020 Psest_1021: Psest_1021 - 3-dehydroquinate dehydratase, type II, at 1,107,600 to 1,108,043 _1021 Psest_1022: Psest_1022 - Thiol:disulfide interchange protein, at 1,108,257 to 1,110,068 _1022 Position (kb) 1107 1108 1109Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1108.172 kb on - strandat 1108.308 kb on + strandat 1108.321 kb on - strandat 1108.343 kb on + strandat 1108.391 kb on - strandat 1108.446 kb on + strand, within Psest_1022at 1108.454 kb on - strand, within Psest_1022at 1108.534 kb on + strand, within Psest_1022at 1108.535 kb on - strand, within Psest_1022at 1108.726 kb on + strand, within Psest_1022at 1108.737 kb on + strand, within Psest_1022at 1108.738 kb on - strand, within Psest_1022at 1108.863 kb on + strand, within Psest_1022at 1108.911 kb on + strand, within Psest_1022at 1108.911 kb on + strand, within Psest_1022at 1108.911 kb on + strand, within Psest_1022at 1108.912 kb on - strand, within Psest_1022at 1108.912 kb on - strand, within Psest_1022

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Per-strain Table

Position Strand Gene LocusTag Fraction Def Chlorate 40 mM
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1,108,172 - -1.5
1,108,308 + -1.2
1,108,321 - -2.6
1,108,343 + -2.4
1,108,391 - +0.2
1,108,446 + Psest_1022 0.10 -0.5
1,108,454 - Psest_1022 0.11 -4.0
1,108,534 + Psest_1022 0.15 -1.5
1,108,535 - Psest_1022 0.15 -2.2
1,108,726 + Psest_1022 0.26 -1.5
1,108,737 + Psest_1022 0.26 -2.0
1,108,738 - Psest_1022 0.27 -1.2
1,108,863 + Psest_1022 0.33 -0.2
1,108,911 + Psest_1022 0.36 +0.8
1,108,911 + Psest_1022 0.36 +1.8
1,108,911 + Psest_1022 0.36 +0.2
1,108,912 - Psest_1022 0.36 -1.6
1,108,912 - Psest_1022 0.36 -1.5

Or see this region's nucleotide sequence