Experiment: Def Chlorate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3028 and Psest_3029 are separated by 58 nucleotides Psest_3029 and Psest_3030 are separated by 76 nucleotides Psest_3030 and Psest_3031 are separated by 44 nucleotides Psest_3031 and Psest_3033 are separated by 349 nucleotides
Psest_3028: Psest_3028 - Protein of unknown function (DUF2946)., at 3,214,760 to 3,215,125
_3028
Psest_3029: Psest_3029 - 3-methyladenine DNA glycosylase, at 3,215,184 to 3,215,855
_3029
Psest_3030: Psest_3030 - phospho-2-dehydro-3-deoxyheptonate aldolase, at 3,215,932 to 3,217,005
_3030
Psest_3031: Psest_3031 - hypothetical protein, at 3,217,050 to 3,217,145
_3031
Psest_3033: Psest_3033 - Predicted ATPase of the PP-loop superfamily implicated in cell cycle control, at 3,217,495 to 3,218,319
_3033
Position (kb)
3215
3216
3217
3218 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3214.976 kb on - strand, within Psest_3028 at 3215.090 kb on + strand at 3215.090 kb on + strand at 3215.091 kb on - strand at 3215.091 kb on - strand at 3215.091 kb on - strand at 3215.136 kb on + strand at 3215.137 kb on - strand at 3215.731 kb on + strand, within Psest_3029 at 3215.827 kb on + strand at 3215.845 kb on + strand at 3215.845 kb on + strand at 3215.846 kb on - strand at 3215.846 kb on - strand at 3215.846 kb on - strand at 3215.846 kb on - strand at 3215.846 kb on - strand at 3215.846 kb on - strand at 3215.846 kb on - strand at 3216.159 kb on + strand, within Psest_3030 at 3216.248 kb on + strand, within Psest_3030 at 3216.248 kb on + strand, within Psest_3030 at 3216.248 kb on + strand, within Psest_3030 at 3216.248 kb on + strand, within Psest_3030 at 3216.249 kb on - strand, within Psest_3030 at 3216.249 kb on - strand, within Psest_3030 at 3216.477 kb on + strand, within Psest_3030 at 3216.478 kb on - strand, within Psest_3030 at 3216.478 kb on - strand, within Psest_3030 at 3216.478 kb on - strand, within Psest_3030 at 3216.478 kb on - strand, within Psest_3030 at 3216.478 kb on - strand, within Psest_3030 at 3216.478 kb on - strand, within Psest_3030 at 3216.518 kb on + strand, within Psest_3030 at 3216.576 kb on + strand, within Psest_3030 at 3216.692 kb on + strand, within Psest_3030 at 3216.692 kb on + strand, within Psest_3030 at 3216.693 kb on - strand, within Psest_3030 at 3216.693 kb on - strand, within Psest_3030 at 3216.759 kb on - strand, within Psest_3030 at 3216.985 kb on + strand at 3216.985 kb on + strand at 3216.985 kb on + strand at 3216.986 kb on - strand at 3216.986 kb on - strand at 3216.986 kb on - strand at 3217.135 kb on + strand, within Psest_3031 at 3217.254 kb on - strand at 3217.270 kb on - strand at 3217.305 kb on + strand at 3217.306 kb on - strand at 3217.306 kb on - strand at 3217.306 kb on - strand at 3217.306 kb on - strand at 3217.308 kb on + strand at 3217.746 kb on - strand, within Psest_3033 at 3217.772 kb on + strand, within Psest_3033 at 3217.940 kb on + strand, within Psest_3033 at 3217.940 kb on + strand, within Psest_3033 at 3217.941 kb on - strand, within Psest_3033 at 3217.941 kb on - strand, within Psest_3033 at 3217.941 kb on - strand, within Psest_3033 at 3217.941 kb on - strand, within Psest_3033
Per-strain Table
Position Strand Gene LocusTag Fraction Def Chlorate 40 mM remove 3,214,976 - Psest_3028 0.59 +0.8 3,215,090 + +1.2 3,215,090 + -1.8 3,215,091 - +1.8 3,215,091 - +1.8 3,215,091 - -0.2 3,215,136 + -0.8 3,215,137 - +1.2 3,215,731 + Psest_3029 0.81 +0.8 3,215,827 + -1.3 3,215,845 + -0.5 3,215,845 + -0.6 3,215,846 - -0.9 3,215,846 - -1.0 3,215,846 - -1.6 3,215,846 - -1.0 3,215,846 - +0.8 3,215,846 - +1.1 3,215,846 - +0.2 3,216,159 + Psest_3030 0.21 -0.4 3,216,248 + Psest_3030 0.29 -2.5 3,216,248 + Psest_3030 0.29 -3.8 3,216,248 + Psest_3030 0.29 -2.8 3,216,248 + Psest_3030 0.29 -2.9 3,216,249 - Psest_3030 0.30 -1.0 3,216,249 - Psest_3030 0.30 -2.8 3,216,477 + Psest_3030 0.51 -1.4 3,216,478 - Psest_3030 0.51 -1.8 3,216,478 - Psest_3030 0.51 -2.0 3,216,478 - Psest_3030 0.51 -1.3 3,216,478 - Psest_3030 0.51 -3.9 3,216,478 - Psest_3030 0.51 -3.2 3,216,478 - Psest_3030 0.51 -1.7 3,216,518 + Psest_3030 0.55 -1.8 3,216,576 + Psest_3030 0.60 -3.8 3,216,692 + Psest_3030 0.71 -1.8 3,216,692 + Psest_3030 0.71 -2.9 3,216,693 - Psest_3030 0.71 -1.3 3,216,693 - Psest_3030 0.71 -2.7 3,216,759 - Psest_3030 0.77 -0.4 3,216,985 + -1.0 3,216,985 + -2.9 3,216,985 + -2.4 3,216,986 - -0.7 3,216,986 - -1.8 3,216,986 - -3.2 3,217,135 + Psest_3031 0.89 -3.4 3,217,254 - +0.1 3,217,270 - -2.1 3,217,305 + -3.8 3,217,306 - -0.8 3,217,306 - -1.3 3,217,306 - -1.9 3,217,306 - -1.4 3,217,308 + -2.3 3,217,746 - Psest_3033 0.30 +0.3 3,217,772 + Psest_3033 0.34 -0.8 3,217,940 + Psest_3033 0.54 -0.6 3,217,940 + Psest_3033 0.54 -0.2 3,217,941 - Psest_3033 0.54 -2.2 3,217,941 - Psest_3033 0.54 -0.7 3,217,941 - Psest_3033 0.54 +0.7 3,217,941 - Psest_3033 0.54 -0.8
Or see this region's nucleotide sequence