Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_1129

Experiment: Def Chlorate 40 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_1127 and Psest_1128 are separated by 120 nucleotidesPsest_1128 and Psest_1129 are separated by 272 nucleotidesPsest_1129 and Psest_1130 are separated by 114 nucleotides Psest_1127: Psest_1127 - hypothetical protein, at 1,222,728 to 1,223,192 _1127 Psest_1128: Psest_1128 - Uncharacterized protein conserved in bacteria, at 1,223,313 to 1,223,768 _1128 Psest_1129: Psest_1129 - preprotein translocase, SecA subunit, at 1,224,041 to 1,226,797 _1129 Psest_1130: Psest_1130 - glutamate N-acetyltransferase/amino-acid acetyltransferase, at 1,226,912 to 1,228,129 _1130 Position (kb) 1224 1225 1226 1227Strain fitness (log2 ratio) -2 -1 0 1 2at 1223.043 kb on + strand, within Psest_1127at 1223.155 kb on - strandat 1223.155 kb on - strandat 1223.303 kb on + strandat 1223.304 kb on - strandat 1223.778 kb on + strandat 1223.778 kb on + strandat 1223.818 kb on - strandat 1223.818 kb on - strandat 1226.768 kb on + strandat 1226.768 kb on + strandat 1226.795 kb on + strandat 1226.796 kb on - strandat 1226.890 kb on + strandat 1226.890 kb on + strandat 1226.890 kb on + strandat 1226.890 kb on + strandat 1226.890 kb on + strandat 1226.891 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Def Chlorate 40 mM
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1,223,043 + Psest_1127 0.68 -0.5
1,223,155 - -1.3
1,223,155 - +0.5
1,223,303 + +0.3
1,223,304 - -0.1
1,223,778 + -2.1
1,223,778 + +0.1
1,223,818 - +2.0
1,223,818 - -0.9
1,226,768 + -1.0
1,226,768 + -2.3
1,226,795 + +0.3
1,226,796 - +1.7
1,226,890 + +0.5
1,226,890 + -0.0
1,226,890 + -0.8
1,226,890 + +0.6
1,226,890 + +0.6
1,226,891 - +1.5

Or see this region's nucleotide sequence