Experiment: Def Chlorate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1127 and Psest_1128 are separated by 120 nucleotides Psest_1128 and Psest_1129 are separated by 272 nucleotides Psest_1129 and Psest_1130 are separated by 114 nucleotides
Psest_1127: Psest_1127 - hypothetical protein, at 1,222,728 to 1,223,192
_1127
Psest_1128: Psest_1128 - Uncharacterized protein conserved in bacteria, at 1,223,313 to 1,223,768
_1128
Psest_1129: Psest_1129 - preprotein translocase, SecA subunit, at 1,224,041 to 1,226,797
_1129
Psest_1130: Psest_1130 - glutamate N-acetyltransferase/amino-acid acetyltransferase, at 1,226,912 to 1,228,129
_1130
Position (kb)
1224
1225
1226
1227 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1223.043 kb on + strand, within Psest_1127 at 1223.155 kb on - strand at 1223.155 kb on - strand at 1223.303 kb on + strand at 1223.304 kb on - strand at 1223.778 kb on + strand at 1223.778 kb on + strand at 1223.818 kb on - strand at 1223.818 kb on - strand at 1226.768 kb on + strand at 1226.768 kb on + strand at 1226.795 kb on + strand at 1226.796 kb on - strand at 1226.890 kb on + strand at 1226.890 kb on + strand at 1226.890 kb on + strand at 1226.890 kb on + strand at 1226.890 kb on + strand at 1226.891 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Chlorate 40 mM remove 1,223,043 + Psest_1127 0.68 -0.5 1,223,155 - -1.3 1,223,155 - +0.5 1,223,303 + +0.3 1,223,304 - -0.1 1,223,778 + -2.1 1,223,778 + +0.1 1,223,818 - +2.0 1,223,818 - -0.9 1,226,768 + -1.0 1,226,768 + -2.3 1,226,795 + +0.3 1,226,796 - +1.7 1,226,890 + +0.5 1,226,890 + -0.0 1,226,890 + -0.8 1,226,890 + +0.6 1,226,890 + +0.6 1,226,891 - +1.5
Or see this region's nucleotide sequence