Experiment: L-Threonine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hsdS-2 and hsdM-2 overlap by 4 nucleotides hsdM-2 and SO4266 are separated by 127 nucleotides
SO4264: hsdS-2 - type I restriction-modification system, S subunit (NCBI ptt file), at 4,439,626 to 4,441,113
hsdS-2
SO4265: hsdM-2 - type I restriction-modification system, M subunit (NCBI ptt file), at 4,441,110 to 4,442,723
hsdM-2
SO4266: SO4266 - conserved hypothetical protein (NCBI ptt file), at 4,442,851 to 4,443,969
SO4266
Position (kb)
4441
4442
4443 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4440.129 kb on + strand, within hsdS-2 at 4440.150 kb on + strand, within hsdS-2 at 4440.150 kb on + strand, within hsdS-2 at 4440.150 kb on + strand, within hsdS-2 at 4440.158 kb on - strand, within hsdS-2 at 4440.236 kb on + strand, within hsdS-2 at 4440.241 kb on + strand, within hsdS-2 at 4440.244 kb on - strand, within hsdS-2 at 4440.245 kb on - strand, within hsdS-2 at 4440.279 kb on + strand, within hsdS-2 at 4440.282 kb on - strand, within hsdS-2 at 4440.307 kb on + strand, within hsdS-2 at 4440.312 kb on + strand, within hsdS-2 at 4440.327 kb on - strand, within hsdS-2 at 4440.332 kb on + strand, within hsdS-2 at 4440.332 kb on + strand, within hsdS-2 at 4440.332 kb on + strand, within hsdS-2 at 4440.332 kb on + strand, within hsdS-2 at 4440.332 kb on + strand, within hsdS-2 at 4440.333 kb on + strand, within hsdS-2 at 4440.335 kb on - strand, within hsdS-2 at 4440.340 kb on - strand, within hsdS-2 at 4440.340 kb on - strand, within hsdS-2 at 4440.343 kb on + strand, within hsdS-2 at 4440.346 kb on - strand, within hsdS-2 at 4440.351 kb on - strand, within hsdS-2 at 4440.356 kb on + strand, within hsdS-2 at 4440.364 kb on - strand, within hsdS-2 at 4440.364 kb on - strand, within hsdS-2 at 4440.381 kb on + strand, within hsdS-2 at 4440.387 kb on - strand, within hsdS-2 at 4440.389 kb on - strand, within hsdS-2 at 4440.419 kb on - strand, within hsdS-2 at 4440.437 kb on + strand, within hsdS-2 at 4440.437 kb on + strand, within hsdS-2 at 4440.437 kb on + strand, within hsdS-2 at 4440.445 kb on - strand, within hsdS-2 at 4440.445 kb on - strand, within hsdS-2 at 4440.447 kb on - strand, within hsdS-2 at 4440.452 kb on - strand, within hsdS-2 at 4440.459 kb on + strand, within hsdS-2 at 4440.581 kb on + strand, within hsdS-2 at 4440.581 kb on + strand, within hsdS-2 at 4440.589 kb on - strand, within hsdS-2 at 4440.591 kb on + strand, within hsdS-2 at 4440.625 kb on - strand, within hsdS-2 at 4440.643 kb on - strand, within hsdS-2 at 4440.652 kb on - strand, within hsdS-2 at 4440.695 kb on + strand, within hsdS-2 at 4440.695 kb on + strand, within hsdS-2 at 4440.695 kb on + strand, within hsdS-2 at 4440.698 kb on - strand, within hsdS-2 at 4440.703 kb on - strand, within hsdS-2 at 4440.706 kb on + strand, within hsdS-2 at 4440.706 kb on + strand, within hsdS-2 at 4440.714 kb on - strand, within hsdS-2 at 4440.714 kb on - strand, within hsdS-2 at 4440.772 kb on - strand, within hsdS-2 at 4440.772 kb on - strand, within hsdS-2 at 4440.791 kb on - strand, within hsdS-2 at 4440.801 kb on - strand, within hsdS-2 at 4440.801 kb on - strand, within hsdS-2 at 4440.806 kb on + strand, within hsdS-2 at 4440.808 kb on - strand, within hsdS-2 at 4440.819 kb on - strand, within hsdS-2 at 4440.823 kb on + strand, within hsdS-2 at 4440.845 kb on - strand, within hsdS-2 at 4440.925 kb on + strand, within hsdS-2 at 4440.925 kb on + strand, within hsdS-2 at 4440.935 kb on + strand, within hsdS-2 at 4440.935 kb on + strand, within hsdS-2 at 4440.935 kb on + strand, within hsdS-2 at 4440.935 kb on + strand, within hsdS-2 at 4440.935 kb on + strand, within hsdS-2 at 4440.935 kb on + strand, within hsdS-2 at 4440.935 kb on + strand, within hsdS-2 at 4440.935 kb on + strand, within hsdS-2 at 4440.935 kb on + strand, within hsdS-2 at 4440.935 kb on + strand, within hsdS-2 at 4440.943 kb on - strand, within hsdS-2 at 4440.943 kb on - strand, within hsdS-2 at 4440.943 kb on - strand, within hsdS-2 at 4440.943 kb on - strand, within hsdS-2 at 4440.943 kb on - strand, within hsdS-2 at 4440.943 kb on - strand, within hsdS-2 at 4440.943 kb on - strand, within hsdS-2 at 4440.943 kb on - strand, within hsdS-2 at 4440.943 kb on - strand, within hsdS-2 at 4440.943 kb on - strand, within hsdS-2 at 4440.943 kb on - strand, within hsdS-2 at 4440.943 kb on - strand, within hsdS-2 at 4440.943 kb on - strand, within hsdS-2 at 4440.943 kb on - strand, within hsdS-2 at 4440.943 kb on - strand, within hsdS-2 at 4440.943 kb on - strand, within hsdS-2 at 4440.943 kb on - strand, within hsdS-2 at 4440.954 kb on + strand, within hsdS-2 at 4440.954 kb on + strand, within hsdS-2 at 4440.956 kb on - strand, within hsdS-2 at 4440.962 kb on - strand, within hsdS-2 at 4440.962 kb on - strand, within hsdS-2 at 4440.995 kb on - strand at 4441.069 kb on + strand at 4441.069 kb on + strand at 4441.069 kb on + strand at 4441.069 kb on + strand at 4441.069 kb on + strand at 4441.069 kb on + strand at 4441.069 kb on + strand at 4441.069 kb on + strand at 4441.069 kb on + strand at 4441.069 kb on + strand at 4441.069 kb on + strand at 4441.077 kb on - strand at 4441.077 kb on - strand at 4441.077 kb on - strand at 4441.077 kb on - strand at 4441.077 kb on - strand at 4441.077 kb on - strand at 4441.077 kb on - strand at 4441.077 kb on - strand at 4441.077 kb on - strand at 4441.077 kb on - strand at 4441.077 kb on - strand at 4441.077 kb on - strand at 4441.087 kb on - strand at 4441.087 kb on - strand at 4441.103 kb on + strand at 4441.116 kb on - strand at 4441.134 kb on - strand at 4441.134 kb on - strand at 4441.134 kb on - strand at 4441.180 kb on + strand at 4441.180 kb on + strand at 4441.180 kb on + strand at 4441.180 kb on + strand at 4441.180 kb on + strand at 4441.188 kb on - strand at 4441.188 kb on - strand at 4441.188 kb on - strand at 4441.188 kb on - strand at 4441.195 kb on - strand at 4441.200 kb on - strand at 4441.212 kb on + strand at 4441.214 kb on - strand at 4441.256 kb on + strand at 4441.277 kb on - strand, within hsdM-2 at 4441.285 kb on + strand, within hsdM-2 at 4441.293 kb on - strand, within hsdM-2 at 4441.341 kb on + strand, within hsdM-2 at 4441.348 kb on + strand, within hsdM-2 at 4441.349 kb on - strand, within hsdM-2 at 4441.411 kb on + strand, within hsdM-2 at 4441.475 kb on - strand, within hsdM-2 at 4441.489 kb on + strand, within hsdM-2 at 4441.568 kb on - strand, within hsdM-2 at 4441.612 kb on + strand, within hsdM-2 at 4441.638 kb on - strand, within hsdM-2 at 4441.645 kb on + strand, within hsdM-2 at 4441.653 kb on - strand, within hsdM-2 at 4441.658 kb on - strand, within hsdM-2 at 4441.684 kb on + strand, within hsdM-2 at 4441.718 kb on - strand, within hsdM-2 at 4441.746 kb on - strand, within hsdM-2 at 4441.789 kb on - strand, within hsdM-2 at 4441.828 kb on - strand, within hsdM-2 at 4441.847 kb on - strand, within hsdM-2 at 4441.927 kb on - strand, within hsdM-2 at 4442.207 kb on + strand, within hsdM-2 at 4442.207 kb on + strand, within hsdM-2 at 4442.231 kb on + strand, within hsdM-2 at 4442.231 kb on + strand, within hsdM-2 at 4442.238 kb on + strand, within hsdM-2 at 4442.238 kb on + strand, within hsdM-2 at 4442.246 kb on - strand, within hsdM-2 at 4442.246 kb on - strand, within hsdM-2 at 4442.246 kb on - strand, within hsdM-2 at 4442.246 kb on - strand, within hsdM-2 at 4442.254 kb on + strand, within hsdM-2 at 4442.262 kb on - strand, within hsdM-2 at 4442.294 kb on - strand, within hsdM-2 at 4442.360 kb on + strand, within hsdM-2 at 4442.368 kb on - strand, within hsdM-2 at 4442.368 kb on - strand, within hsdM-2 at 4442.388 kb on + strand, within hsdM-2 at 4442.462 kb on + strand, within hsdM-2 at 4442.462 kb on + strand, within hsdM-2 at 4442.470 kb on - strand, within hsdM-2 at 4442.509 kb on - strand, within hsdM-2 at 4442.510 kb on - strand, within hsdM-2 at 4442.565 kb on + strand at 4442.668 kb on + strand at 4442.676 kb on - strand at 4442.760 kb on + strand at 4442.766 kb on + strand at 4442.774 kb on - strand at 4442.805 kb on - strand at 4442.809 kb on + strand at 4442.857 kb on - strand at 4442.872 kb on - strand at 4442.911 kb on - strand at 4442.953 kb on - strand at 4442.963 kb on - strand, within SO4266 at 4442.991 kb on + strand, within SO4266 at 4443.045 kb on - strand, within SO4266 at 4443.168 kb on - strand, within SO4266 at 4443.182 kb on + strand, within SO4266 at 4443.189 kb on + strand at 4443.189 kb on + strand, within SO4266 at 4443.190 kb on - strand, within SO4266 at 4443.190 kb on - strand, within SO4266 at 4443.190 kb on - strand, within SO4266 at 4443.190 kb on - strand, within SO4266 at 4443.285 kb on + strand, within SO4266 at 4443.312 kb on - strand, within SO4266 at 4443.350 kb on - strand, within SO4266 at 4443.355 kb on + strand, within SO4266 at 4443.374 kb on + strand, within SO4266 at 4443.383 kb on + strand, within SO4266 at 4443.383 kb on + strand, within SO4266 at 4443.552 kb on + strand, within SO4266 at 4443.575 kb on + strand, within SO4266 at 4443.584 kb on - strand, within SO4266 at 4443.584 kb on - strand, within SO4266 at 4443.644 kb on - strand at 4443.691 kb on - strand, within SO4266
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (N) remove 4,440,129 + hsdS-2 SO4264 0.34 +1.0 4,440,150 + hsdS-2 SO4264 0.35 -0.6 4,440,150 + hsdS-2 SO4264 0.35 +0.7 4,440,150 + hsdS-2 SO4264 0.35 +0.7 4,440,158 - hsdS-2 SO4264 0.36 -0.3 4,440,236 + hsdS-2 SO4264 0.41 +0.7 4,440,241 + hsdS-2 SO4264 0.41 +0.3 4,440,244 - hsdS-2 SO4264 0.42 +0.5 4,440,245 - hsdS-2 SO4264 0.42 +0.4 4,440,279 + hsdS-2 SO4264 0.44 +1.1 4,440,282 - hsdS-2 SO4264 0.44 +0.1 4,440,307 + hsdS-2 SO4264 0.46 +0.4 4,440,312 + hsdS-2 SO4264 0.46 -1.8 4,440,327 - hsdS-2 SO4264 0.47 +0.4 4,440,332 + hsdS-2 SO4264 0.47 -0.1 4,440,332 + hsdS-2 SO4264 0.47 +0.3 4,440,332 + hsdS-2 SO4264 0.47 +0.7 4,440,332 + hsdS-2 SO4264 0.47 -0.5 4,440,332 + hsdS-2 SO4264 0.47 +0.9 4,440,333 + hsdS-2 SO4264 0.48 +0.8 4,440,335 - hsdS-2 SO4264 0.48 +1.1 4,440,340 - hsdS-2 SO4264 0.48 +0.0 4,440,340 - hsdS-2 SO4264 0.48 +0.4 4,440,343 + hsdS-2 SO4264 0.48 -0.0 4,440,346 - hsdS-2 SO4264 0.48 +0.0 4,440,351 - hsdS-2 SO4264 0.49 -0.0 4,440,356 + hsdS-2 SO4264 0.49 +0.2 4,440,364 - hsdS-2 SO4264 0.50 +0.8 4,440,364 - hsdS-2 SO4264 0.50 +0.5 4,440,381 + hsdS-2 SO4264 0.51 +0.2 4,440,387 - hsdS-2 SO4264 0.51 +0.3 4,440,389 - hsdS-2 SO4264 0.51 +0.7 4,440,419 - hsdS-2 SO4264 0.53 -0.4 4,440,437 + hsdS-2 SO4264 0.55 -0.7 4,440,437 + hsdS-2 SO4264 0.55 +0.9 4,440,437 + hsdS-2 SO4264 0.55 -0.0 4,440,445 - hsdS-2 SO4264 0.55 -0.6 4,440,445 - hsdS-2 SO4264 0.55 -0.4 4,440,447 - hsdS-2 SO4264 0.55 +0.9 4,440,452 - hsdS-2 SO4264 0.56 -0.0 4,440,459 + hsdS-2 SO4264 0.56 +1.0 4,440,581 + hsdS-2 SO4264 0.64 +1.0 4,440,581 + hsdS-2 SO4264 0.64 -0.6 4,440,589 - hsdS-2 SO4264 0.65 +0.5 4,440,591 + hsdS-2 SO4264 0.65 +0.2 4,440,625 - hsdS-2 SO4264 0.67 -2.3 4,440,643 - hsdS-2 SO4264 0.68 -1.0 4,440,652 - hsdS-2 SO4264 0.69 +0.4 4,440,695 + hsdS-2 SO4264 0.72 +0.8 4,440,695 + hsdS-2 SO4264 0.72 -1.1 4,440,695 + hsdS-2 SO4264 0.72 +0.4 4,440,698 - hsdS-2 SO4264 0.72 +0.8 4,440,703 - hsdS-2 SO4264 0.72 +0.2 4,440,706 + hsdS-2 SO4264 0.73 +0.1 4,440,706 + hsdS-2 SO4264 0.73 +0.0 4,440,714 - hsdS-2 SO4264 0.73 -0.4 4,440,714 - hsdS-2 SO4264 0.73 -1.3 4,440,772 - hsdS-2 SO4264 0.77 -1.7 4,440,772 - hsdS-2 SO4264 0.77 +0.4 4,440,791 - hsdS-2 SO4264 0.78 +0.0 4,440,801 - hsdS-2 SO4264 0.79 -0.9 4,440,801 - hsdS-2 SO4264 0.79 +0.8 4,440,806 + hsdS-2 SO4264 0.79 +0.9 4,440,808 - hsdS-2 SO4264 0.79 +0.5 4,440,819 - hsdS-2 SO4264 0.80 -0.6 4,440,823 + hsdS-2 SO4264 0.80 +1.0 4,440,845 - hsdS-2 SO4264 0.82 -0.7 4,440,925 + hsdS-2 SO4264 0.87 +1.0 4,440,925 + hsdS-2 SO4264 0.87 +1.3 4,440,935 + hsdS-2 SO4264 0.88 +0.5 4,440,935 + hsdS-2 SO4264 0.88 -1.0 4,440,935 + hsdS-2 SO4264 0.88 +0.0 4,440,935 + hsdS-2 SO4264 0.88 -0.6 4,440,935 + hsdS-2 SO4264 0.88 +2.1 4,440,935 + hsdS-2 SO4264 0.88 +1.5 4,440,935 + hsdS-2 SO4264 0.88 +0.4 4,440,935 + hsdS-2 SO4264 0.88 +0.9 4,440,935 + hsdS-2 SO4264 0.88 +0.5 4,440,935 + hsdS-2 SO4264 0.88 +0.4 4,440,943 - hsdS-2 SO4264 0.89 +1.3 4,440,943 - hsdS-2 SO4264 0.89 -0.3 4,440,943 - hsdS-2 SO4264 0.89 -1.5 4,440,943 - hsdS-2 SO4264 0.89 -0.1 4,440,943 - hsdS-2 SO4264 0.89 +0.3 4,440,943 - hsdS-2 SO4264 0.89 +1.4 4,440,943 - hsdS-2 SO4264 0.89 -0.3 4,440,943 - hsdS-2 SO4264 0.89 +0.2 4,440,943 - hsdS-2 SO4264 0.89 -0.2 4,440,943 - hsdS-2 SO4264 0.89 +1.7 4,440,943 - hsdS-2 SO4264 0.89 -1.1 4,440,943 - hsdS-2 SO4264 0.89 +0.2 4,440,943 - hsdS-2 SO4264 0.89 +0.4 4,440,943 - hsdS-2 SO4264 0.89 +1.4 4,440,943 - hsdS-2 SO4264 0.89 -1.3 4,440,943 - hsdS-2 SO4264 0.89 -0.8 4,440,943 - hsdS-2 SO4264 0.89 +0.3 4,440,954 + hsdS-2 SO4264 0.89 +0.7 4,440,954 + hsdS-2 SO4264 0.89 +0.4 4,440,956 - hsdS-2 SO4264 0.89 -0.6 4,440,962 - hsdS-2 SO4264 0.90 +1.2 4,440,962 - hsdS-2 SO4264 0.90 +0.9 4,440,995 - -0.9 4,441,069 + +0.1 4,441,069 + -0.9 4,441,069 + +1.0 4,441,069 + +0.5 4,441,069 + -0.1 4,441,069 + -0.5 4,441,069 + +0.2 4,441,069 + -0.1 4,441,069 + +0.3 4,441,069 + -0.2 4,441,069 + +1.8 4,441,077 - -0.2 4,441,077 - -0.1 4,441,077 - +0.5 4,441,077 - -1.9 4,441,077 - +0.4 4,441,077 - -0.2 4,441,077 - +0.9 4,441,077 - -0.7 4,441,077 - +0.5 4,441,077 - -0.9 4,441,077 - +0.1 4,441,077 - +0.7 4,441,087 - -1.5 4,441,087 - +0.4 4,441,103 + +0.1 4,441,116 - -0.7 4,441,134 - -1.0 4,441,134 - +1.6 4,441,134 - -0.5 4,441,180 + -0.9 4,441,180 + -0.0 4,441,180 + +0.6 4,441,180 + -0.3 4,441,180 + -0.7 4,441,188 - -0.1 4,441,188 - -1.8 4,441,188 - +0.0 4,441,188 - +1.8 4,441,195 - -0.3 4,441,200 - +0.7 4,441,212 + -0.3 4,441,214 - -1.8 4,441,256 + +0.1 4,441,277 - hsdM-2 SO4265 0.10 +0.1 4,441,285 + hsdM-2 SO4265 0.11 +0.5 4,441,293 - hsdM-2 SO4265 0.11 -0.7 4,441,341 + hsdM-2 SO4265 0.14 -0.4 4,441,348 + hsdM-2 SO4265 0.15 -0.5 4,441,349 - hsdM-2 SO4265 0.15 +0.5 4,441,411 + hsdM-2 SO4265 0.19 +0.2 4,441,475 - hsdM-2 SO4265 0.23 -0.7 4,441,489 + hsdM-2 SO4265 0.23 +0.7 4,441,568 - hsdM-2 SO4265 0.28 -1.7 4,441,612 + hsdM-2 SO4265 0.31 -1.5 4,441,638 - hsdM-2 SO4265 0.33 +0.5 4,441,645 + hsdM-2 SO4265 0.33 +0.2 4,441,653 - hsdM-2 SO4265 0.34 -0.3 4,441,658 - hsdM-2 SO4265 0.34 -0.7 4,441,684 + hsdM-2 SO4265 0.36 -0.2 4,441,718 - hsdM-2 SO4265 0.38 -0.4 4,441,746 - hsdM-2 SO4265 0.39 +0.2 4,441,789 - hsdM-2 SO4265 0.42 +1.0 4,441,828 - hsdM-2 SO4265 0.44 +0.7 4,441,847 - hsdM-2 SO4265 0.46 -1.5 4,441,927 - hsdM-2 SO4265 0.51 +0.2 4,442,207 + hsdM-2 SO4265 0.68 -0.0 4,442,207 + hsdM-2 SO4265 0.68 +0.9 4,442,231 + hsdM-2 SO4265 0.69 -0.2 4,442,231 + hsdM-2 SO4265 0.69 +0.2 4,442,238 + hsdM-2 SO4265 0.70 -0.6 4,442,238 + hsdM-2 SO4265 0.70 -0.7 4,442,246 - hsdM-2 SO4265 0.70 +0.3 4,442,246 - hsdM-2 SO4265 0.70 +0.3 4,442,246 - hsdM-2 SO4265 0.70 -0.4 4,442,246 - hsdM-2 SO4265 0.70 -2.1 4,442,254 + hsdM-2 SO4265 0.71 -0.1 4,442,262 - hsdM-2 SO4265 0.71 +0.7 4,442,294 - hsdM-2 SO4265 0.73 +1.0 4,442,360 + hsdM-2 SO4265 0.77 +1.0 4,442,368 - hsdM-2 SO4265 0.78 +1.2 4,442,368 - hsdM-2 SO4265 0.78 -0.5 4,442,388 + hsdM-2 SO4265 0.79 +0.2 4,442,462 + hsdM-2 SO4265 0.84 -0.2 4,442,462 + hsdM-2 SO4265 0.84 +0.7 4,442,470 - hsdM-2 SO4265 0.84 +0.0 4,442,509 - hsdM-2 SO4265 0.87 +0.8 4,442,510 - hsdM-2 SO4265 0.87 -2.3 4,442,565 + +0.1 4,442,668 + +0.8 4,442,676 - -2.3 4,442,760 + +0.3 4,442,766 + +0.6 4,442,774 - -0.4 4,442,805 - +0.7 4,442,809 + -0.7 4,442,857 - -0.3 4,442,872 - +1.0 4,442,911 - +0.9 4,442,953 - -0.3 4,442,963 - SO4266 0.10 -0.9 4,442,991 + SO4266 0.13 -0.4 4,443,045 - SO4266 0.17 -0.5 4,443,168 - SO4266 0.28 +0.1 4,443,182 + SO4266 0.30 -1.0 4,443,189 + +0.1 4,443,189 + SO4266 0.30 +0.5 4,443,190 - SO4266 0.30 +0.8 4,443,190 - SO4266 0.30 -0.5 4,443,190 - SO4266 0.30 -0.2 4,443,190 - SO4266 0.30 -3.9 4,443,285 + SO4266 0.39 +0.9 4,443,312 - SO4266 0.41 -1.1 4,443,350 - SO4266 0.45 +1.2 4,443,355 + SO4266 0.45 +0.2 4,443,374 + SO4266 0.47 -0.0 4,443,383 + SO4266 0.48 -0.3 4,443,383 + SO4266 0.48 +1.1 4,443,552 + SO4266 0.63 +0.1 4,443,575 + SO4266 0.65 +1.0 4,443,584 - SO4266 0.66 +0.1 4,443,584 - SO4266 0.66 -0.3 4,443,644 - +1.1 4,443,691 - SO4266 0.75 -0.5
Or see this region's nucleotide sequence