Experiment: L-Threonine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3339 and SO3340 are separated by 31 nucleotides SO3340 and SO3341 are separated by 111 nucleotides SO3341 and SO3342 are separated by 36 nucleotides
SO3339: SO3339 - OmpA family protein (NCBI ptt file), at 3,486,976 to 3,487,566
SO3339
SO3340: SO3340 - hypothetical Small-conductance mechanosensitive channel (NCBI ptt file), at 3,487,598 to 3,488,425
SO3340
SO3341: SO3341 - antioxidant, AhpC/TSA family (NCBI ptt file), at 3,488,537 to 3,489,130
SO3341
SO3342: SO3342 - putative transporter, required for L-alanine utilization (from data) , at 3,489,167 to 3,489,808
SO3342
Position (kb)
3488
3489
3490 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3487.625 kb on - strand at 3487.629 kb on + strand at 3487.629 kb on + strand at 3487.637 kb on - strand at 3487.653 kb on - strand at 3487.655 kb on - strand at 3487.660 kb on + strand at 3487.712 kb on + strand, within SO3340 at 3487.712 kb on + strand, within SO3340 at 3487.720 kb on - strand, within SO3340 at 3487.732 kb on - strand, within SO3340 at 3487.735 kb on + strand, within SO3340 at 3487.735 kb on + strand, within SO3340 at 3487.735 kb on + strand, within SO3340 at 3487.735 kb on + strand, within SO3340 at 3487.741 kb on - strand, within SO3340 at 3487.763 kb on - strand, within SO3340 at 3487.832 kb on - strand, within SO3340 at 3487.891 kb on + strand, within SO3340 at 3487.893 kb on + strand, within SO3340 at 3487.893 kb on + strand, within SO3340 at 3487.893 kb on + strand, within SO3340 at 3487.901 kb on + strand, within SO3340 at 3487.901 kb on + strand, within SO3340 at 3487.901 kb on + strand, within SO3340 at 3487.901 kb on + strand, within SO3340 at 3487.901 kb on - strand, within SO3340 at 3487.901 kb on - strand, within SO3340 at 3487.901 kb on - strand, within SO3340 at 3487.907 kb on + strand, within SO3340 at 3487.909 kb on - strand, within SO3340 at 3487.909 kb on - strand, within SO3340 at 3487.921 kb on + strand, within SO3340 at 3487.923 kb on - strand, within SO3340 at 3487.943 kb on + strand, within SO3340 at 3487.943 kb on - strand, within SO3340 at 3488.001 kb on - strand, within SO3340 at 3488.038 kb on - strand, within SO3340 at 3488.062 kb on - strand, within SO3340 at 3488.063 kb on + strand, within SO3340 at 3488.071 kb on - strand, within SO3340 at 3488.093 kb on + strand, within SO3340 at 3488.103 kb on + strand, within SO3340 at 3488.106 kb on - strand, within SO3340 at 3488.154 kb on - strand, within SO3340 at 3488.203 kb on + strand, within SO3340 at 3488.211 kb on - strand, within SO3340 at 3488.222 kb on + strand, within SO3340 at 3488.227 kb on + strand, within SO3340 at 3488.227 kb on + strand, within SO3340 at 3488.227 kb on + strand, within SO3340 at 3488.235 kb on - strand, within SO3340 at 3488.235 kb on - strand, within SO3340 at 3488.243 kb on - strand, within SO3340 at 3488.348 kb on + strand at 3488.410 kb on + strand at 3488.494 kb on + strand at 3488.494 kb on + strand at 3488.494 kb on + strand at 3488.502 kb on - strand at 3488.502 kb on - strand at 3488.502 kb on - strand at 3488.524 kb on - strand at 3488.595 kb on + strand at 3488.603 kb on - strand, within SO3341 at 3488.603 kb on - strand, within SO3341 at 3488.609 kb on + strand, within SO3341 at 3488.626 kb on + strand, within SO3341 at 3488.636 kb on + strand, within SO3341 at 3488.660 kb on + strand, within SO3341 at 3488.702 kb on - strand, within SO3341 at 3488.766 kb on + strand, within SO3341 at 3488.766 kb on + strand, within SO3341 at 3488.774 kb on - strand, within SO3341 at 3488.774 kb on - strand, within SO3341 at 3488.774 kb on - strand, within SO3341 at 3488.774 kb on - strand, within SO3341 at 3488.779 kb on - strand, within SO3341 at 3488.786 kb on - strand, within SO3341 at 3488.815 kb on + strand, within SO3341 at 3488.852 kb on + strand, within SO3341 at 3488.857 kb on - strand, within SO3341 at 3488.873 kb on + strand, within SO3341 at 3488.873 kb on + strand, within SO3341 at 3488.873 kb on + strand, within SO3341 at 3488.873 kb on + strand, within SO3341 at 3488.873 kb on + strand, within SO3341 at 3488.873 kb on + strand, within SO3341 at 3488.881 kb on - strand, within SO3341 at 3488.881 kb on - strand, within SO3341 at 3488.881 kb on - strand, within SO3341 at 3488.881 kb on - strand, within SO3341 at 3488.881 kb on - strand, within SO3341 at 3488.881 kb on - strand, within SO3341 at 3488.881 kb on - strand, within SO3341 at 3488.881 kb on - strand, within SO3341 at 3488.881 kb on - strand, within SO3341 at 3488.881 kb on - strand, within SO3341 at 3488.881 kb on - strand, within SO3341 at 3488.881 kb on - strand, within SO3341 at 3488.881 kb on - strand, within SO3341 at 3488.938 kb on - strand, within SO3341 at 3488.968 kb on - strand, within SO3341 at 3489.045 kb on + strand, within SO3341 at 3489.194 kb on - strand at 3489.194 kb on - strand at 3489.217 kb on + strand at 3489.317 kb on + strand, within SO3342 at 3489.325 kb on - strand, within SO3342 at 3489.325 kb on - strand, within SO3342 at 3489.431 kb on + strand, within SO3342 at 3489.447 kb on + strand, within SO3342 at 3489.490 kb on + strand, within SO3342 at 3489.508 kb on + strand, within SO3342 at 3489.621 kb on + strand, within SO3342 at 3489.685 kb on - strand, within SO3342 at 3489.721 kb on + strand, within SO3342 at 3489.740 kb on + strand, within SO3342 at 3489.948 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (N) remove 3,487,625 - +0.6 3,487,629 + +0.1 3,487,629 + +0.7 3,487,637 - +1.5 3,487,653 - +1.1 3,487,655 - +1.4 3,487,660 + +1.5 3,487,712 + SO3340 0.14 +1.5 3,487,712 + SO3340 0.14 +0.6 3,487,720 - SO3340 0.15 +1.3 3,487,732 - SO3340 0.16 +0.7 3,487,735 + SO3340 0.17 +0.0 3,487,735 + SO3340 0.17 -2.7 3,487,735 + SO3340 0.17 +1.2 3,487,735 + SO3340 0.17 +0.6 3,487,741 - SO3340 0.17 +0.3 3,487,763 - SO3340 0.20 +1.0 3,487,832 - SO3340 0.28 +0.7 3,487,891 + SO3340 0.35 -0.5 3,487,893 + SO3340 0.36 -0.4 3,487,893 + SO3340 0.36 +0.3 3,487,893 + SO3340 0.36 +0.3 3,487,901 + SO3340 0.37 -1.3 3,487,901 + SO3340 0.37 +0.6 3,487,901 + SO3340 0.37 +0.8 3,487,901 + SO3340 0.37 +0.5 3,487,901 - SO3340 0.37 +0.8 3,487,901 - SO3340 0.37 +0.9 3,487,901 - SO3340 0.37 +1.6 3,487,907 + SO3340 0.37 +1.0 3,487,909 - SO3340 0.38 +2.0 3,487,909 - SO3340 0.38 -0.2 3,487,921 + SO3340 0.39 -1.7 3,487,923 - SO3340 0.39 +1.8 3,487,943 + SO3340 0.42 -0.5 3,487,943 - SO3340 0.42 +0.3 3,488,001 - SO3340 0.49 +0.1 3,488,038 - SO3340 0.53 +0.1 3,488,062 - SO3340 0.56 +1.8 3,488,063 + SO3340 0.56 +0.4 3,488,071 - SO3340 0.57 +2.2 3,488,093 + SO3340 0.60 +1.3 3,488,103 + SO3340 0.61 +0.6 3,488,106 - SO3340 0.61 +1.2 3,488,154 - SO3340 0.67 +0.6 3,488,203 + SO3340 0.73 +1.2 3,488,211 - SO3340 0.74 +0.4 3,488,222 + SO3340 0.75 +0.1 3,488,227 + SO3340 0.76 +0.2 3,488,227 + SO3340 0.76 +0.2 3,488,227 + SO3340 0.76 +1.2 3,488,235 - SO3340 0.77 -0.6 3,488,235 - SO3340 0.77 +0.7 3,488,243 - SO3340 0.78 +1.7 3,488,348 + -0.9 3,488,410 + +0.8 3,488,494 + -0.1 3,488,494 + +0.1 3,488,494 + +0.3 3,488,502 - +0.6 3,488,502 - +1.4 3,488,502 - -0.4 3,488,524 - +0.4 3,488,595 + -0.3 3,488,603 - SO3341 0.11 -0.3 3,488,603 - SO3341 0.11 +0.9 3,488,609 + SO3341 0.12 +0.7 3,488,626 + SO3341 0.15 +0.1 3,488,636 + SO3341 0.17 +0.8 3,488,660 + SO3341 0.21 +0.4 3,488,702 - SO3341 0.28 -2.4 3,488,766 + SO3341 0.39 -1.3 3,488,766 + SO3341 0.39 +2.0 3,488,774 - SO3341 0.40 -3.0 3,488,774 - SO3341 0.40 +0.1 3,488,774 - SO3341 0.40 +0.5 3,488,774 - SO3341 0.40 -1.6 3,488,779 - SO3341 0.41 +0.4 3,488,786 - SO3341 0.42 -0.3 3,488,815 + SO3341 0.47 -0.4 3,488,852 + SO3341 0.53 -0.6 3,488,857 - SO3341 0.54 -1.7 3,488,873 + SO3341 0.57 -0.9 3,488,873 + SO3341 0.57 +0.2 3,488,873 + SO3341 0.57 -0.7 3,488,873 + SO3341 0.57 +1.0 3,488,873 + SO3341 0.57 -0.9 3,488,873 + SO3341 0.57 +0.6 3,488,881 - SO3341 0.58 -0.9 3,488,881 - SO3341 0.58 -0.3 3,488,881 - SO3341 0.58 +0.2 3,488,881 - SO3341 0.58 -0.5 3,488,881 - SO3341 0.58 -0.3 3,488,881 - SO3341 0.58 -0.5 3,488,881 - SO3341 0.58 +1.1 3,488,881 - SO3341 0.58 -0.8 3,488,881 - SO3341 0.58 -0.9 3,488,881 - SO3341 0.58 -0.5 3,488,881 - SO3341 0.58 -2.2 3,488,881 - SO3341 0.58 +0.1 3,488,881 - SO3341 0.58 +1.4 3,488,938 - SO3341 0.68 -0.0 3,488,968 - SO3341 0.73 +0.0 3,489,045 + SO3341 0.86 -0.3 3,489,194 - +1.5 3,489,194 - +1.3 3,489,217 + +1.3 3,489,317 + SO3342 0.23 +2.6 3,489,325 - SO3342 0.25 +1.2 3,489,325 - SO3342 0.25 +1.0 3,489,431 + SO3342 0.41 +1.9 3,489,447 + SO3342 0.44 +1.9 3,489,490 + SO3342 0.50 -0.1 3,489,508 + SO3342 0.53 +1.2 3,489,621 + SO3342 0.71 +1.8 3,489,685 - SO3342 0.81 +0.7 3,489,721 + SO3342 0.86 +1.8 3,489,740 + SO3342 0.89 +2.5 3,489,948 - -0.2
Or see this region's nucleotide sequence