Strain Fitness in Shewanella oneidensis MR-1 around SO2832

Experiment: L-Threonine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSO2830 and ribA are separated by 42 nucleotidesribA and SO2832 are separated by 262 nucleotidesSO2832 and nrdG are separated by 125 nucleotidesnrdG and nrdD are separated by 115 nucleotides SO2830: SO2830 - hypothetical protein (NCBI ptt file), at 2,948,015 to 2,948,953 SO2830 SO2831: ribA - GTP cyclohydrolase II (NCBI ptt file), at 2,948,996 to 2,949,607 ribA SO2832: SO2832 - conserved hypothetical protein (NCBI ptt file), at 2,949,870 to 2,950,226 SO2832 SO2833: nrdG - anaerobic ribonucleoside-triphosphate reductase activating protein (NCBI ptt file), at 2,950,352 to 2,950,813 nrdG SO2834: nrdD - anaerobic ribonucleoside-triphosphate reductase (NCBI ptt file), at 2,950,929 to 2,953,046 nrdD Position (kb) 2949 2950 2951Strain fitness (log2 ratio) -2 -1 0 1 2at 2948.920 kb on + strandat 2948.961 kb on - strandat 2949.707 kb on - strandat 2949.790 kb on - strandat 2949.921 kb on + strand, within SO2832at 2949.921 kb on + strand, within SO2832at 2949.929 kb on - strand, within SO2832at 2949.929 kb on - strand, within SO2832at 2949.978 kb on + strand, within SO2832at 2949.978 kb on + strand, within SO2832at 2949.986 kb on - strand, within SO2832at 2949.986 kb on - strand, within SO2832at 2949.986 kb on - strand, within SO2832at 2950.122 kb on - strand, within SO2832at 2950.122 kb on - strand, within SO2832at 2950.263 kb on + strandat 2950.263 kb on + strandat 2950.263 kb on + strandat 2950.271 kb on - strandat 2950.271 kb on - strandat 2950.271 kb on - strandat 2950.301 kb on + strandat 2950.317 kb on - strandat 2950.459 kb on - strand, within nrdGat 2950.459 kb on - strand, within nrdGat 2950.541 kb on - strand, within nrdGat 2950.693 kb on + strand, within nrdGat 2950.866 kb on + strandat 2950.866 kb on + strandat 2950.891 kb on + strandat 2950.937 kb on + strandat 2950.974 kb on + strandat 2951.075 kb on - strandat 2951.145 kb on + strand, within nrdD

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Threonine (N)
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2,948,920 + +0.8
2,948,961 - -0.1
2,949,707 - +1.1
2,949,790 - +0.8
2,949,921 + SO2832 0.14 +2.1
2,949,921 + SO2832 0.14 +2.0
2,949,929 - SO2832 0.17 +0.8
2,949,929 - SO2832 0.17 +0.5
2,949,978 + SO2832 0.30 +2.3
2,949,978 + SO2832 0.30 +2.4
2,949,986 - SO2832 0.32 +2.5
2,949,986 - SO2832 0.32 +1.3
2,949,986 - SO2832 0.32 +1.0
2,950,122 - SO2832 0.71 +0.3
2,950,122 - SO2832 0.71 +2.7
2,950,263 + +1.7
2,950,263 + +2.0
2,950,263 + +0.8
2,950,271 - +0.8
2,950,271 - +0.1
2,950,271 - -0.1
2,950,301 + +2.3
2,950,317 - +1.3
2,950,459 - nrdG SO2833 0.23 -2.2
2,950,459 - nrdG SO2833 0.23 -0.3
2,950,541 - nrdG SO2833 0.41 +0.2
2,950,693 + nrdG SO2833 0.74 +0.6
2,950,866 + +0.0
2,950,866 + +0.1
2,950,891 + -0.7
2,950,937 + +0.1
2,950,974 + +0.5
2,951,075 - +1.0
2,951,145 + nrdD SO2834 0.10 -0.1

Or see this region's nucleotide sequence