Strain Fitness in Shewanella oneidensis MR-1 around SO2774

Experiment: L-Threonine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSO2772 and SO2773 are separated by 116 nucleotidesSO2773 and fabF-1 are separated by 177 nucleotidesfabF-1 and acpP are separated by 209 nucleotidesacpP and fabG-1 are separated by 163 nucleotides SO2772: SO2772 - cytosolic long-chain acyl-CoA thioester hydrolase family protein (NCBI ptt file), at 2,895,188 to 2,895,667 SO2772 SO2773: SO2773 - hypothetical protein (NCBI ptt file), at 2,895,784 to 2,896,152 SO2773 SO2774: fabF-1 - 3-oxoacyl-(acyl-carrier-protein) synthase II (NCBI ptt file), at 2,896,330 to 2,897,568 fabF-1 SO2775: acpP - acyl carrier protein (NCBI ptt file), at 2,897,778 to 2,898,011 acpP SO2776: fabG-1 - 3-oxoacyl-(acyl-carrier-protein) reductase (NCBI ptt file), at 2,898,175 to 2,898,921 fabG-1 Position (kb) 2896 2897 2898Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2895.530 kb on + strand, within SO2772at 2895.548 kb on + strand, within SO2772at 2895.548 kb on + strand, within SO2772at 2895.548 kb on + strand, within SO2772at 2895.556 kb on - strand, within SO2772at 2895.556 kb on - strand, within SO2772at 2895.588 kb on + strand, within SO2772at 2895.938 kb on - strand, within SO2773at 2895.992 kb on + strand, within SO2773at 2896.128 kb on - strandat 2896.381 kb on + strandat 2896.392 kb on - strandat 2896.927 kb on - strand, within fabF-1at 2896.979 kb on - strand, within fabF-1at 2897.280 kb on - strand, within fabF-1at 2897.285 kb on - strand, within fabF-1at 2897.301 kb on - strand, within fabF-1at 2897.360 kb on - strand, within fabF-1at 2897.473 kb on - strandat 2897.485 kb on - strandat 2897.532 kb on + strandat 2897.600 kb on + strandat 2898.140 kb on + strandat 2898.180 kb on - strandat 2898.180 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Threonine (N)
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2,895,530 + SO2772 0.71 -2.5
2,895,548 + SO2772 0.75 -2.0
2,895,548 + SO2772 0.75 -1.6
2,895,548 + SO2772 0.75 +0.5
2,895,556 - SO2772 0.77 -1.1
2,895,556 - SO2772 0.77 -2.4
2,895,588 + SO2772 0.83 -1.8
2,895,938 - SO2773 0.42 +0.2
2,895,992 + SO2773 0.56 -1.6
2,896,128 - +0.4
2,896,381 + +2.5
2,896,392 - +1.9
2,896,927 - fabF-1 SO2774 0.48 +1.6
2,896,979 - fabF-1 SO2774 0.52 +1.8
2,897,280 - fabF-1 SO2774 0.77 +0.0
2,897,285 - fabF-1 SO2774 0.77 +0.7
2,897,301 - fabF-1 SO2774 0.78 +1.2
2,897,360 - fabF-1 SO2774 0.83 +2.9
2,897,473 - +3.3
2,897,485 - +2.6
2,897,532 + +2.2
2,897,600 + +0.8
2,898,140 + +1.6
2,898,180 - +1.9
2,898,180 - +2.3

Or see this region's nucleotide sequence