Strain Fitness in Shewanella oneidensis MR-1 around SO1525

Experiment: L-Threonine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSO1523 and grpE are separated by 99 nucleotidesgrpE and dxs are separated by 117 nucleotidesdxs and ispA are separated by 21 nucleotidesispA and SO_1527 overlap by 7 nucleotides SO1523: SO1523 - conserved hypothetical protein (NCBI ptt file), at 1,599,467 to 1,600,345 SO1523 SO1524: grpE - heat shock protein GrpE (NCBI ptt file), at 1,600,445 to 1,601,065 grpE SO1525: dxs - deoxyxylulose-5-phosphate synthase (NCBI ptt file), at 1,601,183 to 1,603,051 dxs SO1526: ispA - geranyltranstransferase (NCBI ptt file), at 1,603,073 to 1,603,954 ispA SO_1527: SO_1527 - no description, at 1,603,948 to 1,604,196 _1527 Position (kb) 1601 1602 1603 1604Strain fitness (log2 ratio) -1 0 1 2 3at 1601.014 kb on + strandat 1601.066 kb on + strandat 1601.199 kb on + strandat 1601.207 kb on - strandat 1601.207 kb on - strandat 1601.207 kb on - strandat 1601.207 kb on - strandat 1604.042 kb on + strand, within SO_1527

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Threonine (N)
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1,601,014 + +0.9
1,601,066 + +1.5
1,601,199 + -1.1
1,601,207 - +0.9
1,601,207 - -0.1
1,601,207 - -0.2
1,601,207 - -1.0
1,604,042 + SO_1527 0.38 +3.2

Or see this region's nucleotide sequence