Experiment: L-Threonine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0357 and SO0358 are separated by 199 nucleotides SO0358 and spoT are separated by 24 nucleotides
SO0357: SO0357 - metallo-beta-lactamase superfamily protein (NCBI ptt file), at 365,794 to 367,647
SO0357
SO0358: SO0358 - endoribonuclease L-PSP, putative (NCBI ptt file), at 367,847 to 368,230
SO0358
SO0359: spoT - guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (NCBI ptt file), at 368,255 to 370,360
spoT
Position (kb) 367
368
369 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 366.906 kb on + strand, within SO0357 at 367.120 kb on - strand, within SO0357 at 367.177 kb on + strand, within SO0357 at 367.199 kb on - strand, within SO0357 at 367.237 kb on - strand, within SO0357 at 367.443 kb on - strand, within SO0357 at 367.465 kb on - strand at 367.640 kb on + strand at 367.765 kb on + strand at 367.783 kb on - strand at 367.815 kb on - strand at 367.845 kb on + strand at 367.845 kb on + strand at 367.897 kb on + strand, within SO0358 at 367.905 kb on - strand, within SO0358 at 367.923 kb on + strand, within SO0358 at 367.923 kb on + strand, within SO0358 at 367.931 kb on - strand, within SO0358 at 367.931 kb on - strand, within SO0358 at 367.931 kb on - strand, within SO0358 at 367.976 kb on + strand, within SO0358 at 367.983 kb on - strand, within SO0358 at 367.984 kb on - strand, within SO0358 at 367.984 kb on - strand, within SO0358 at 368.363 kb on + strand at 368.388 kb on - strand at 368.496 kb on - strand, within spoT at 368.581 kb on + strand, within spoT at 368.588 kb on + strand, within spoT at 368.875 kb on + strand, within spoT at 368.883 kb on - strand, within spoT at 368.898 kb on - strand, within spoT at 368.928 kb on - strand, within spoT at 368.930 kb on + strand, within spoT
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (N) remove 366,906 + SO0357 0.60 +0.0 367,120 - SO0357 0.72 +0.1 367,177 + SO0357 0.75 -0.1 367,199 - SO0357 0.76 -1.7 367,237 - SO0357 0.78 -0.6 367,443 - SO0357 0.89 -0.7 367,465 - +3.3 367,640 + +1.0 367,765 + +0.6 367,783 - -0.2 367,815 - -2.1 367,845 + +0.7 367,845 + +0.6 367,897 + SO0358 0.13 -2.7 367,905 - SO0358 0.15 -2.6 367,923 + SO0358 0.20 +0.8 367,923 + SO0358 0.20 -2.6 367,931 - SO0358 0.22 -4.1 367,931 - SO0358 0.22 -4.4 367,931 - SO0358 0.22 -3.7 367,976 + SO0358 0.34 -2.4 367,983 - SO0358 0.35 -3.6 367,984 - SO0358 0.36 -4.9 367,984 - SO0358 0.36 -3.5 368,363 + -2.7 368,388 - -0.6 368,496 - spoT SO0359 0.11 -0.3 368,581 + spoT SO0359 0.15 -0.6 368,588 + spoT SO0359 0.16 -3.5 368,875 + spoT SO0359 0.29 -2.8 368,883 - spoT SO0359 0.30 -1.8 368,898 - spoT SO0359 0.31 +3.3 368,928 - spoT SO0359 0.32 -4.4 368,930 + spoT SO0359 0.32 -1.3
Or see this region's nucleotide sequence