Experiment: L-Threonine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt argH and mrcA are separated by 74 nucleotides mrcA and SO0281 are separated by 189 nucleotides
SO0279: argH - argininosuccinate lyase (NCBI ptt file), at 284,611 to 285,978
argH
SO0280: mrcA - penicillin-binding protein 1A (NCBI ptt file), at 286,053 to 288,572
mrcA
SO0281: SO0281 - type IV pilus biogenesis protein PilM (NCBI ptt file), at 288,762 to 289,841
SO0281
Position (kb) 286
287
288
289 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 285.133 kb on - strand, within argH at 285.154 kb on - strand, within argH at 285.236 kb on + strand, within argH at 285.244 kb on - strand, within argH at 285.261 kb on - strand, within argH at 285.283 kb on + strand, within argH at 285.319 kb on + strand, within argH at 285.322 kb on - strand, within argH at 285.324 kb on + strand, within argH at 285.342 kb on - strand, within argH at 285.403 kb on + strand, within argH at 285.403 kb on + strand, within argH at 285.403 kb on + strand, within argH at 285.403 kb on + strand, within argH at 285.505 kb on + strand, within argH at 285.587 kb on - strand, within argH at 285.623 kb on + strand, within argH at 285.669 kb on + strand, within argH at 285.669 kb on + strand, within argH at 285.696 kb on + strand, within argH at 285.735 kb on + strand, within argH at 285.784 kb on - strand, within argH at 285.821 kb on + strand, within argH at 285.837 kb on + strand, within argH at 285.846 kb on - strand at 285.908 kb on + strand at 285.939 kb on - strand at 286.059 kb on + strand at 288.616 kb on - strand at 288.631 kb on - strand at 288.631 kb on - strand at 288.650 kb on + strand at 288.722 kb on - strand at 288.805 kb on + strand at 288.845 kb on + strand at 288.845 kb on + strand at 288.845 kb on + strand at 288.952 kb on + strand, within SO0281 at 288.960 kb on - strand, within SO0281 at 289.042 kb on - strand, within SO0281 at 289.103 kb on + strand, within SO0281 at 289.190 kb on + strand, within SO0281 at 289.190 kb on + strand, within SO0281 at 289.193 kb on - strand, within SO0281 at 289.193 kb on - strand, within SO0281 at 289.198 kb on - strand, within SO0281 at 289.198 kb on - strand, within SO0281 at 289.198 kb on - strand, within SO0281 at 289.198 kb on - strand, within SO0281 at 289.198 kb on - strand, within SO0281 at 289.203 kb on - strand, within SO0281 at 289.223 kb on - strand, within SO0281 at 289.272 kb on + strand, within SO0281 at 289.279 kb on + strand, within SO0281 at 289.280 kb on - strand, within SO0281 at 289.284 kb on + strand, within SO0281 at 289.284 kb on + strand, within SO0281 at 289.284 kb on + strand, within SO0281 at 289.311 kb on - strand, within SO0281 at 289.332 kb on + strand, within SO0281 at 289.338 kb on - strand, within SO0281 at 289.392 kb on + strand, within SO0281 at 289.410 kb on + strand, within SO0281 at 289.410 kb on + strand, within SO0281 at 289.422 kb on + strand, within SO0281 at 289.428 kb on + strand, within SO0281 at 289.436 kb on - strand, within SO0281 at 289.436 kb on - strand, within SO0281 at 289.436 kb on - strand, within SO0281 at 289.454 kb on + strand, within SO0281 at 289.550 kb on - strand, within SO0281
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (N) remove 285,133 - argH SO0279 0.38 -3.5 285,154 - argH SO0279 0.40 -1.7 285,236 + argH SO0279 0.46 -5.4 285,244 - argH SO0279 0.46 -2.7 285,261 - argH SO0279 0.48 -4.7 285,283 + argH SO0279 0.49 -2.8 285,319 + argH SO0279 0.52 -0.9 285,322 - argH SO0279 0.52 -4.5 285,324 + argH SO0279 0.52 -4.8 285,342 - argH SO0279 0.53 -4.3 285,403 + argH SO0279 0.58 -2.7 285,403 + argH SO0279 0.58 -0.8 285,403 + argH SO0279 0.58 -4.9 285,403 + argH SO0279 0.58 -4.6 285,505 + argH SO0279 0.65 -2.8 285,587 - argH SO0279 0.71 -0.2 285,623 + argH SO0279 0.74 -4.1 285,669 + argH SO0279 0.77 -5.1 285,669 + argH SO0279 0.77 -5.0 285,696 + argH SO0279 0.79 -3.9 285,735 + argH SO0279 0.82 -3.3 285,784 - argH SO0279 0.86 -2.4 285,821 + argH SO0279 0.88 -2.3 285,837 + argH SO0279 0.90 -3.2 285,846 - -6.1 285,908 + -5.3 285,939 - -1.8 286,059 + +0.9 288,616 - -0.5 288,631 - -0.3 288,631 - +0.6 288,650 + +0.2 288,722 - +0.6 288,805 + +0.2 288,845 + +1.8 288,845 + -1.2 288,845 + -1.0 288,952 + SO0281 0.18 +0.0 288,960 - SO0281 0.18 -0.1 289,042 - SO0281 0.26 -0.2 289,103 + SO0281 0.32 -0.1 289,190 + SO0281 0.40 -0.2 289,190 + SO0281 0.40 +0.4 289,193 - SO0281 0.40 -0.3 289,193 - SO0281 0.40 +1.1 289,198 - SO0281 0.40 +0.8 289,198 - SO0281 0.40 -0.7 289,198 - SO0281 0.40 -0.3 289,198 - SO0281 0.40 +0.1 289,198 - SO0281 0.40 -0.3 289,203 - SO0281 0.41 -0.0 289,223 - SO0281 0.43 +1.8 289,272 + SO0281 0.47 +0.0 289,279 + SO0281 0.48 +0.8 289,280 - SO0281 0.48 -0.1 289,284 + SO0281 0.48 +0.2 289,284 + SO0281 0.48 -2.0 289,284 + SO0281 0.48 -0.7 289,311 - SO0281 0.51 -0.0 289,332 + SO0281 0.53 +0.5 289,338 - SO0281 0.53 -1.0 289,392 + SO0281 0.58 -0.1 289,410 + SO0281 0.60 -0.9 289,410 + SO0281 0.60 -2.1 289,422 + SO0281 0.61 -0.5 289,428 + SO0281 0.62 -0.1 289,436 - SO0281 0.62 -1.1 289,436 - SO0281 0.62 +0.3 289,436 - SO0281 0.62 +0.8 289,454 + SO0281 0.64 -0.5 289,550 - SO0281 0.73 -0.3
Or see this region's nucleotide sequence