Experiment: L-Threonine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ppc and argC are separated by 698 nucleotides argC and argB are separated by 31 nucleotides argB and argF are separated by 12 nucleotides
SO0274: ppc - phosphoenolpyruvate carboxylase (NCBI ptt file), at 277,180 to 279,825
ppc
SO0275: argC - N-acetyl-gamma-glutamyl-phosphate reductase (NCBI ptt file), at 280,524 to 281,504
argC
SO0276: argB - acetylglutamate kinase (NCBI ptt file), at 281,536 to 282,318
argB
SO0277: argF - ornithine carbamoyltransferase (NCBI ptt file), at 282,331 to 283,236
argF
Position (kb) 280
281
282 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 279.550 kb on - strand, within ppc at 279.651 kb on + strand at 279.659 kb on - strand at 279.772 kb on + strand at 279.886 kb on + strand at 279.894 kb on - strand at 279.967 kb on + strand at 280.213 kb on - strand at 280.341 kb on + strand at 280.341 kb on - strand at 280.349 kb on - strand at 280.379 kb on + strand at 280.384 kb on + strand at 280.392 kb on - strand at 280.461 kb on - strand at 280.553 kb on + strand at 280.571 kb on + strand at 280.578 kb on + strand at 280.579 kb on - strand at 280.579 kb on - strand at 280.586 kb on - strand at 280.586 kb on - strand at 280.615 kb on - strand at 280.623 kb on - strand, within argC at 280.662 kb on + strand, within argC at 280.670 kb on - strand, within argC at 280.670 kb on - strand, within argC at 280.674 kb on + strand, within argC at 280.674 kb on + strand, within argC at 280.674 kb on + strand, within argC at 280.674 kb on + strand, within argC at 280.674 kb on + strand, within argC at 280.682 kb on - strand, within argC at 280.689 kb on + strand at 280.697 kb on - strand, within argC at 280.704 kb on - strand, within argC at 280.755 kb on - strand, within argC at 280.757 kb on + strand, within argC at 280.820 kb on - strand, within argC at 280.837 kb on + strand, within argC at 280.882 kb on - strand, within argC at 280.977 kb on + strand, within argC at 281.045 kb on - strand, within argC at 281.047 kb on + strand, within argC at 281.047 kb on + strand, within argC at 281.055 kb on - strand, within argC at 281.067 kb on - strand, within argC at 281.080 kb on - strand, within argC at 281.125 kb on + strand, within argC at 281.125 kb on + strand, within argC at 281.133 kb on - strand, within argC at 281.133 kb on - strand, within argC at 281.142 kb on + strand, within argC at 281.250 kb on - strand, within argC at 281.255 kb on + strand, within argC at 281.255 kb on + strand, within argC at 281.309 kb on - strand, within argC at 281.359 kb on + strand, within argC at 281.421 kb on + strand at 281.469 kb on + strand at 281.732 kb on - strand, within argB at 281.880 kb on + strand, within argB at 281.880 kb on + strand, within argB at 282.002 kb on + strand, within argB at 282.010 kb on - strand, within argB at 282.103 kb on + strand, within argB at 282.133 kb on + strand, within argB at 282.214 kb on + strand, within argB at 282.455 kb on - strand, within argF
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (N) remove 279,550 - ppc SO0274 0.90 -1.7 279,651 + -3.4 279,659 - -2.2 279,772 + -1.7 279,886 + -4.3 279,894 - +0.5 279,967 + -4.1 280,213 - +0.2 280,341 + -5.1 280,341 - -5.3 280,349 - -4.7 280,379 + -1.5 280,384 + -3.7 280,392 - -2.2 280,461 - -3.5 280,553 + -1.5 280,571 + -4.5 280,578 + -4.1 280,579 - -3.6 280,579 - -1.1 280,586 - -4.9 280,586 - -3.4 280,615 - -3.6 280,623 - argC SO0275 0.10 -2.7 280,662 + argC SO0275 0.14 -5.4 280,670 - argC SO0275 0.15 -5.0 280,670 - argC SO0275 0.15 -3.3 280,674 + argC SO0275 0.15 -2.5 280,674 + argC SO0275 0.15 -4.6 280,674 + argC SO0275 0.15 -5.0 280,674 + argC SO0275 0.15 -5.4 280,674 + argC SO0275 0.15 -4.9 280,682 - argC SO0275 0.16 -6.0 280,689 + +1.6 280,697 - argC SO0275 0.18 -3.2 280,704 - argC SO0275 0.18 -1.7 280,755 - argC SO0275 0.24 -3.7 280,757 + argC SO0275 0.24 -3.0 280,820 - argC SO0275 0.30 -4.9 280,837 + argC SO0275 0.32 -3.3 280,882 - argC SO0275 0.36 -4.7 280,977 + argC SO0275 0.46 -2.5 281,045 - argC SO0275 0.53 -1.5 281,047 + argC SO0275 0.53 -3.1 281,047 + argC SO0275 0.53 -4.2 281,055 - argC SO0275 0.54 -3.5 281,067 - argC SO0275 0.55 -2.9 281,080 - argC SO0275 0.57 -1.9 281,125 + argC SO0275 0.61 -2.4 281,125 + argC SO0275 0.61 -2.0 281,133 - argC SO0275 0.62 -4.5 281,133 - argC SO0275 0.62 -4.4 281,142 + argC SO0275 0.63 -1.4 281,250 - argC SO0275 0.74 -5.3 281,255 + argC SO0275 0.75 -1.4 281,255 + argC SO0275 0.75 -4.3 281,309 - argC SO0275 0.80 -3.0 281,359 + argC SO0275 0.85 -3.0 281,421 + -5.2 281,469 + -2.7 281,732 - argB SO0276 0.25 -4.3 281,880 + argB SO0276 0.44 -1.9 281,880 + argB SO0276 0.44 -3.6 282,002 + argB SO0276 0.60 -4.4 282,010 - argB SO0276 0.61 -3.7 282,103 + argB SO0276 0.72 -3.8 282,133 + argB SO0276 0.76 -3.9 282,214 + argB SO0276 0.87 -2.9 282,455 - argF SO0277 0.14 -3.8
Or see this region's nucleotide sequence