Experiment: Def Chlorate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1013 and Psest_1014 are separated by 199 nucleotides Psest_1014 and Psest_1015 are separated by 76 nucleotides Psest_1015 and Psest_1016 overlap by 4 nucleotides
Psest_1013: Psest_1013 - phosphoribosylamine--glycine ligase, at 1,098,615 to 1,099,907
_1013
Psest_1014: Psest_1014 - phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase, at 1,100,107 to 1,101,711
_1014
Psest_1015: Psest_1015 - Factor for inversion stimulation Fis, transcriptional activator, at 1,101,788 to 1,102,108
_1015
Psest_1016: Psest_1016 - putative TIM-barrel protein, nifR3 family, at 1,102,105 to 1,103,118
_1016
Position (kb)
1100
1101
1102 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1099.114 kb on + strand, within Psest_1013 at 1099.114 kb on + strand, within Psest_1013 at 1099.115 kb on - strand, within Psest_1013 at 1099.179 kb on - strand, within Psest_1013 at 1099.213 kb on + strand, within Psest_1013 at 1099.214 kb on - strand, within Psest_1013 at 1099.510 kb on + strand, within Psest_1013 at 1099.510 kb on + strand, within Psest_1013 at 1099.510 kb on + strand, within Psest_1013 at 1099.510 kb on + strand, within Psest_1013 at 1099.510 kb on + strand, within Psest_1013 at 1099.510 kb on + strand, within Psest_1013 at 1099.547 kb on - strand, within Psest_1013 at 1099.899 kb on + strand at 1099.899 kb on + strand at 1099.899 kb on + strand at 1099.899 kb on + strand at 1099.899 kb on + strand at 1099.900 kb on - strand at 1099.900 kb on - strand at 1099.900 kb on - strand at 1099.900 kb on - strand at 1099.900 kb on - strand at 1099.900 kb on - strand at 1099.972 kb on + strand at 1099.979 kb on - strand at 1100.001 kb on + strand at 1100.001 kb on + strand at 1100.001 kb on + strand at 1100.002 kb on - strand at 1100.002 kb on - strand at 1100.002 kb on - strand at 1100.108 kb on + strand at 1101.204 kb on - strand, within Psest_1014 at 1102.083 kb on - strand at 1102.254 kb on - strand, within Psest_1016 at 1102.302 kb on - strand, within Psest_1016 at 1102.302 kb on - strand, within Psest_1016 at 1102.392 kb on - strand, within Psest_1016 at 1102.392 kb on - strand, within Psest_1016 at 1102.563 kb on - strand, within Psest_1016 at 1102.563 kb on - strand, within Psest_1016 at 1102.580 kb on + strand, within Psest_1016 at 1102.581 kb on - strand, within Psest_1016 at 1102.581 kb on - strand, within Psest_1016
Per-strain Table
Position Strand Gene LocusTag Fraction Def Chlorate 20 mM remove 1,099,114 + Psest_1013 0.39 -0.2 1,099,114 + Psest_1013 0.39 -1.5 1,099,115 - Psest_1013 0.39 -2.7 1,099,179 - Psest_1013 0.44 -0.9 1,099,213 + Psest_1013 0.46 -1.7 1,099,214 - Psest_1013 0.46 -0.9 1,099,510 + Psest_1013 0.69 -1.6 1,099,510 + Psest_1013 0.69 -0.1 1,099,510 + Psest_1013 0.69 +0.1 1,099,510 + Psest_1013 0.69 -0.9 1,099,510 + Psest_1013 0.69 -0.2 1,099,510 + Psest_1013 0.69 +0.1 1,099,547 - Psest_1013 0.72 -0.6 1,099,899 + -1.7 1,099,899 + -0.1 1,099,899 + -2.3 1,099,899 + -1.5 1,099,899 + -1.7 1,099,900 - -2.6 1,099,900 - -1.2 1,099,900 - -2.2 1,099,900 - -2.2 1,099,900 - -0.7 1,099,900 - -1.2 1,099,972 + -2.0 1,099,979 - -0.0 1,100,001 + -3.4 1,100,001 + -1.1 1,100,001 + +0.9 1,100,002 - +0.3 1,100,002 - -1.1 1,100,002 - +0.4 1,100,108 + +0.1 1,101,204 - Psest_1014 0.68 -0.9 1,102,083 - -0.8 1,102,254 - Psest_1016 0.15 +0.4 1,102,302 - Psest_1016 0.19 -1.4 1,102,302 - Psest_1016 0.19 -0.7 1,102,392 - Psest_1016 0.28 -0.9 1,102,392 - Psest_1016 0.28 -1.7 1,102,563 - Psest_1016 0.45 -0.6 1,102,563 - Psest_1016 0.45 +0.2 1,102,580 + Psest_1016 0.47 -0.0 1,102,581 - Psest_1016 0.47 -1.0 1,102,581 - Psest_1016 0.47 +0.4
Or see this region's nucleotide sequence