Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0009

Experiment: Def Chlorate 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0007 and Psest_0008 are separated by 50 nucleotidesPsest_0008 and Psest_0009 overlap by 4 nucleotidesPsest_0009 and Psest_0010 overlap by 32 nucleotides Psest_0007: Psest_0007 - diguanylate cyclase (GGDEF) domain, at 8,642 to 10,012 _0007 Psest_0008: Psest_0008 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 10,063 to 10,812 _0008 Psest_0009: Psest_0009 - histidinol-phosphate phosphatase family domain/HAD-superfamily hydrolase, subfamily IIIA, at 10,809 to 11,387 _0009 Psest_0010: Psest_0010 - glycyl-tRNA synthetase, tetrameric type, beta subunit, at 11,356 to 13,410 _0010 Position (kb) 10 11 12Strain fitness (log2 ratio) -2 -1 0 1at 9.883 kb on - strandat 9.883 kb on - strandat 10.001 kb on + strandat 10.002 kb on - strandat 10.002 kb on - strandat 10.002 kb on - strandat 10.002 kb on - strandat 10.002 kb on - strandat 10.013 kb on - strandat 10.828 kb on - strandat 10.828 kb on - strandat 10.828 kb on - strandat 11.358 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Def Chlorate 20 mM
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9,883 - -0.5
9,883 - +0.1
10,001 + +0.3
10,002 - +0.9
10,002 - -0.5
10,002 - +0.6
10,002 - -1.0
10,002 - +0.9
10,013 - -0.4
10,828 - +0.2
10,828 - +1.5
10,828 - -1.8
11,358 - +0.5

Or see this region's nucleotide sequence