Experiment: Def Chlorate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0059 and Psest_0060 are separated by 2 nucleotides Psest_0060 and Psest_0061 are separated by 118 nucleotides Psest_0061 and Psest_0062 are separated by 175 nucleotides Psest_0062 and Psest_0063 overlap by 4 nucleotides
Psest_0059: Psest_0059 - hypothetical protein, at 60,782 to 61,153
_0059
Psest_0060: Psest_0060 - Predicted Fe-S protein, at 61,156 to 61,338
_0060
Psest_0061: Psest_0061 - hypothetical protein, at 61,457 to 61,786
_0061
Psest_0062: Psest_0062 - tryptophan synthase, alpha subunit, at 61,962 to 62,771
_0062
Psest_0063: Psest_0063 - tryptophan synthase, beta subunit, at 62,768 to 63,979
_0063
Position (kb) 61
62
63 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 61.215 kb on + strand, within Psest_0060 at 61.216 kb on - strand, within Psest_0060 at 61.290 kb on - strand, within Psest_0060 at 61.416 kb on + strand at 61.417 kb on - strand at 61.530 kb on + strand, within Psest_0061 at 61.530 kb on + strand, within Psest_0061 at 61.530 kb on + strand, within Psest_0061 at 61.531 kb on - strand, within Psest_0061 at 61.641 kb on + strand, within Psest_0061 at 61.653 kb on + strand, within Psest_0061 at 61.896 kb on + strand at 61.897 kb on - strand at 61.900 kb on + strand at 61.900 kb on + strand at 61.900 kb on + strand at 61.901 kb on - strand at 61.901 kb on - strand at 61.901 kb on - strand at 61.901 kb on - strand at 61.901 kb on - strand at 61.980 kb on + strand at 61.980 kb on + strand at 61.981 kb on - strand at 62.245 kb on + strand, within Psest_0062 at 62.245 kb on + strand, within Psest_0062 at 62.245 kb on + strand, within Psest_0062 at 62.245 kb on + strand, within Psest_0062 at 62.246 kb on - strand, within Psest_0062 at 62.246 kb on - strand, within Psest_0062 at 62.246 kb on - strand, within Psest_0062 at 62.246 kb on - strand, within Psest_0062 at 62.246 kb on - strand, within Psest_0062 at 62.248 kb on + strand, within Psest_0062 at 62.249 kb on - strand, within Psest_0062 at 62.249 kb on - strand, within Psest_0062 at 62.249 kb on - strand, within Psest_0062 at 62.464 kb on + strand, within Psest_0062 at 62.464 kb on + strand, within Psest_0062 at 62.464 kb on + strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.684 kb on - strand, within Psest_0062 at 62.992 kb on - strand, within Psest_0063 at 62.992 kb on - strand, within Psest_0063 at 63.405 kb on + strand, within Psest_0063 at 63.405 kb on + strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.564 kb on + strand, within Psest_0063 at 63.565 kb on - strand, within Psest_0063 at 63.565 kb on - strand, within Psest_0063 at 63.565 kb on - strand, within Psest_0063 at 63.565 kb on - strand, within Psest_0063 at 63.565 kb on - strand, within Psest_0063 at 63.741 kb on + strand, within Psest_0063 at 63.741 kb on + strand, within Psest_0063 at 63.741 kb on + strand, within Psest_0063 at 63.741 kb on + strand, within Psest_0063 at 63.741 kb on + strand, within Psest_0063 at 63.742 kb on - strand, within Psest_0063 at 63.742 kb on - strand, within Psest_0063 at 63.742 kb on - strand, within Psest_0063 at 63.742 kb on - strand, within Psest_0063
Per-strain Table
Position Strand Gene LocusTag Fraction Def Chlorate 20 mM remove 61,215 + Psest_0060 0.32 -0.9 61,216 - Psest_0060 0.33 -1.8 61,290 - Psest_0060 0.73 -1.0 61,416 + +0.0 61,417 - +0.0 61,530 + Psest_0061 0.22 -0.3 61,530 + Psest_0061 0.22 +0.7 61,530 + Psest_0061 0.22 +3.3 61,531 - Psest_0061 0.22 +0.1 61,641 + Psest_0061 0.56 -0.8 61,653 + Psest_0061 0.59 -1.0 61,896 + +0.7 61,897 - +0.0 61,900 + -1.1 61,900 + +0.7 61,900 + +1.3 61,901 - -0.5 61,901 - -0.4 61,901 - -0.6 61,901 - +1.8 61,901 - +0.3 61,980 + +1.0 61,980 + +1.7 61,981 - +2.8 62,245 + Psest_0062 0.35 -0.6 62,245 + Psest_0062 0.35 -1.8 62,245 + Psest_0062 0.35 -2.1 62,245 + Psest_0062 0.35 -1.9 62,246 - Psest_0062 0.35 +1.3 62,246 - Psest_0062 0.35 -2.4 62,246 - Psest_0062 0.35 -0.5 62,246 - Psest_0062 0.35 +0.3 62,246 - Psest_0062 0.35 -1.1 62,248 + Psest_0062 0.35 -2.0 62,249 - Psest_0062 0.35 +0.0 62,249 - Psest_0062 0.35 +0.3 62,249 - Psest_0062 0.35 -0.8 62,464 + Psest_0062 0.62 -2.9 62,464 + Psest_0062 0.62 -3.3 62,464 + Psest_0062 0.62 -4.5 62,465 - Psest_0062 0.62 -1.1 62,465 - Psest_0062 0.62 -1.3 62,465 - Psest_0062 0.62 -3.3 62,465 - Psest_0062 0.62 -2.1 62,465 - Psest_0062 0.62 -1.3 62,465 - Psest_0062 0.62 -0.7 62,465 - Psest_0062 0.62 +1.3 62,465 - Psest_0062 0.62 -1.0 62,465 - Psest_0062 0.62 -1.9 62,465 - Psest_0062 0.62 +2.3 62,465 - Psest_0062 0.62 -1.8 62,465 - Psest_0062 0.62 +0.3 62,684 - Psest_0062 0.89 -4.2 62,992 - Psest_0063 0.18 -0.6 62,992 - Psest_0063 0.18 -2.5 63,405 + Psest_0063 0.53 +1.3 63,405 + Psest_0063 0.53 -0.8 63,406 - Psest_0063 0.53 -1.7 63,406 - Psest_0063 0.53 -1.4 63,406 - Psest_0063 0.53 -2.2 63,406 - Psest_0063 0.53 -2.1 63,406 - Psest_0063 0.53 +0.3 63,406 - Psest_0063 0.53 +1.3 63,406 - Psest_0063 0.53 +1.3 63,406 - Psest_0063 0.53 -0.5 63,564 + Psest_0063 0.66 -1.8 63,565 - Psest_0063 0.66 +0.3 63,565 - Psest_0063 0.66 -2.8 63,565 - Psest_0063 0.66 -0.5 63,565 - Psest_0063 0.66 -1.1 63,565 - Psest_0063 0.66 -1.9 63,741 + Psest_0063 0.80 -2.6 63,741 + Psest_0063 0.80 +0.3 63,741 + Psest_0063 0.80 +1.3 63,741 + Psest_0063 0.80 -0.7 63,741 + Psest_0063 0.80 -1.5 63,742 - Psest_0063 0.80 -3.0 63,742 - Psest_0063 0.80 -1.7 63,742 - Psest_0063 0.80 +0.0 63,742 - Psest_0063 0.80 -0.5
Or see this region's nucleotide sequence