Experiment: Def Chlorate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2537 and Psest_2538 are separated by 106 nucleotides Psest_2538 and Psest_2539 are separated by 24 nucleotides Psest_2539 and Psest_2540 are separated by 54 nucleotides Psest_2540 and Psest_2541 are separated by 27 nucleotides
Psest_2537: Psest_2537 - oxygen-independent coproporphyrinogen III oxidase, at 2,717,609 to 2,718,991
_2537
Psest_2538: Psest_2538 - cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases, at 2,719,098 to 2,719,832
_2538
Psest_2539: Psest_2539 - adenine phosphoribosyltransferase, at 2,719,857 to 2,720,405
_2539
Psest_2540: Psest_2540 - Uncharacterized protein SCO1/SenC/PrrC, involved in biogenesis of respiratory and photosynthetic systems, at 2,720,460 to 2,721,068
_2540
Psest_2541: Psest_2541 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 2,721,096 to 2,721,797
_2541
Position (kb)
2719
2720
2721 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2718.947 kb on + strand at 2718.948 kb on - strand at 2718.962 kb on - strand at 2718.989 kb on + strand at 2719.028 kb on - strand at 2719.058 kb on + strand at 2719.072 kb on - strand at 2719.097 kb on + strand at 2719.097 kb on + strand at 2719.097 kb on + strand at 2719.097 kb on + strand at 2719.097 kb on + strand at 2719.097 kb on + strand at 2719.097 kb on + strand at 2719.098 kb on - strand at 2719.098 kb on - strand at 2719.098 kb on - strand at 2719.098 kb on - strand at 2719.098 kb on - strand at 2719.098 kb on - strand at 2719.098 kb on - strand at 2719.256 kb on - strand, within Psest_2538 at 2719.313 kb on + strand, within Psest_2538 at 2719.774 kb on + strand at 2719.775 kb on - strand at 2719.892 kb on - strand at 2719.940 kb on - strand, within Psest_2539 at 2720.145 kb on + strand, within Psest_2539 at 2720.145 kb on + strand, within Psest_2539 at 2720.293 kb on + strand, within Psest_2539 at 2720.294 kb on - strand, within Psest_2539 at 2720.432 kb on - strand at 2720.454 kb on + strand at 2720.455 kb on - strand at 2720.455 kb on - strand at 2720.567 kb on - strand, within Psest_2540 at 2720.567 kb on - strand, within Psest_2540 at 2720.663 kb on - strand, within Psest_2540 at 2720.702 kb on + strand, within Psest_2540 at 2720.728 kb on + strand, within Psest_2540 at 2720.770 kb on + strand, within Psest_2540 at 2720.820 kb on + strand, within Psest_2540 at 2720.820 kb on + strand, within Psest_2540 at 2720.820 kb on + strand, within Psest_2540 at 2720.821 kb on - strand, within Psest_2540 at 2720.821 kb on - strand, within Psest_2540 at 2720.821 kb on - strand, within Psest_2540 at 2720.821 kb on - strand, within Psest_2540 at 2720.821 kb on - strand, within Psest_2540 at 2720.821 kb on - strand, within Psest_2540 at 2720.829 kb on + strand, within Psest_2540 at 2720.830 kb on - strand, within Psest_2540 at 2720.830 kb on - strand, within Psest_2540 at 2720.830 kb on - strand, within Psest_2540 at 2720.830 kb on - strand, within Psest_2540 at 2720.830 kb on - strand, within Psest_2540 at 2720.830 kb on - strand, within Psest_2540 at 2720.830 kb on - strand, within Psest_2540 at 2720.833 kb on - strand, within Psest_2540 at 2720.938 kb on - strand, within Psest_2540 at 2720.938 kb on - strand, within Psest_2540 at 2720.938 kb on - strand, within Psest_2540 at 2721.113 kb on + strand at 2721.123 kb on + strand at 2721.124 kb on - strand at 2721.124 kb on - strand at 2721.127 kb on + strand at 2721.127 kb on + strand at 2721.127 kb on + strand at 2721.128 kb on - strand at 2721.128 kb on - strand at 2721.128 kb on - strand at 2721.128 kb on - strand at 2721.128 kb on - strand at 2721.128 kb on - strand at 2721.128 kb on - strand at 2721.128 kb on - strand at 2721.128 kb on - strand at 2721.209 kb on + strand, within Psest_2541 at 2721.210 kb on - strand, within Psest_2541 at 2721.210 kb on - strand, within Psest_2541 at 2721.252 kb on + strand, within Psest_2541 at 2721.252 kb on + strand, within Psest_2541 at 2721.273 kb on - strand, within Psest_2541 at 2721.352 kb on - strand, within Psest_2541
Per-strain Table
Position Strand Gene LocusTag Fraction Def Chlorate 20 mM remove 2,718,947 + +1.9 2,718,948 - -0.4 2,718,962 - +0.8 2,718,989 + +1.0 2,719,028 - +1.4 2,719,058 + +1.2 2,719,072 - +2.5 2,719,097 + +3.2 2,719,097 + +2.6 2,719,097 + +2.7 2,719,097 + +3.2 2,719,097 + +3.9 2,719,097 + +2.7 2,719,097 + +2.2 2,719,098 - +3.9 2,719,098 - +2.3 2,719,098 - +2.7 2,719,098 - +3.3 2,719,098 - +2.6 2,719,098 - +2.8 2,719,098 - +2.7 2,719,256 - Psest_2538 0.21 +4.1 2,719,313 + Psest_2538 0.29 +3.9 2,719,774 + +4.0 2,719,775 - +4.0 2,719,892 - +0.6 2,719,940 - Psest_2539 0.15 +0.4 2,720,145 + Psest_2539 0.52 -0.4 2,720,145 + Psest_2539 0.52 +0.1 2,720,293 + Psest_2539 0.79 -0.2 2,720,294 - Psest_2539 0.80 -0.5 2,720,432 - -0.7 2,720,454 + -0.2 2,720,455 - +2.4 2,720,455 - -2.9 2,720,567 - Psest_2540 0.18 +0.0 2,720,567 - Psest_2540 0.18 -0.8 2,720,663 - Psest_2540 0.33 +1.2 2,720,702 + Psest_2540 0.40 -0.9 2,720,728 + Psest_2540 0.44 -0.2 2,720,770 + Psest_2540 0.51 +1.4 2,720,820 + Psest_2540 0.59 +0.1 2,720,820 + Psest_2540 0.59 +0.2 2,720,820 + Psest_2540 0.59 -0.1 2,720,821 - Psest_2540 0.59 +1.0 2,720,821 - Psest_2540 0.59 -0.3 2,720,821 - Psest_2540 0.59 +0.8 2,720,821 - Psest_2540 0.59 -0.5 2,720,821 - Psest_2540 0.59 -1.4 2,720,821 - Psest_2540 0.59 +0.5 2,720,829 + Psest_2540 0.61 +0.7 2,720,830 - Psest_2540 0.61 -1.5 2,720,830 - Psest_2540 0.61 +0.8 2,720,830 - Psest_2540 0.61 -0.5 2,720,830 - Psest_2540 0.61 +1.3 2,720,830 - Psest_2540 0.61 +0.1 2,720,830 - Psest_2540 0.61 +1.1 2,720,830 - Psest_2540 0.61 -0.4 2,720,833 - Psest_2540 0.61 +0.2 2,720,938 - Psest_2540 0.78 -0.0 2,720,938 - Psest_2540 0.78 +1.0 2,720,938 - Psest_2540 0.78 -0.6 2,721,113 + +0.8 2,721,123 + +0.8 2,721,124 - -2.0 2,721,124 - -0.3 2,721,127 + +1.0 2,721,127 + +1.7 2,721,127 + +0.2 2,721,128 - -0.1 2,721,128 - +0.4 2,721,128 - -0.7 2,721,128 - -0.5 2,721,128 - +1.1 2,721,128 - +0.3 2,721,128 - +1.3 2,721,128 - -2.1 2,721,128 - +0.3 2,721,209 + Psest_2541 0.16 +0.6 2,721,210 - Psest_2541 0.16 +0.1 2,721,210 - Psest_2541 0.16 +1.8 2,721,252 + Psest_2541 0.22 +1.6 2,721,252 + Psest_2541 0.22 -0.6 2,721,273 - Psest_2541 0.25 -0.6 2,721,352 - Psest_2541 0.36 +0.1
Or see this region's nucleotide sequence