Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_2019

Experiment: Def Chlorate 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_2018 and Psest_2019 are separated by 76 nucleotidesPsest_2019 and Psest_2020 are separated by 87 nucleotidesPsest_2020 and Psest_2021 overlap by 4 nucleotides Psest_2018: Psest_2018 - Uncharacterized conserved protein, at 2,136,821 to 2,137,987 _2018 Psest_2019: Psest_2019 - adenylosuccinate lyase, at 2,138,064 to 2,139,434 _2019 Psest_2020: Psest_2020 - Uncharacterized protein involved in purine metabolism, at 2,139,522 to 2,140,160 _2020 Psest_2021: Psest_2021 - tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase, at 2,140,157 to 2,141,284 _2021 Position (kb) 2138 2139 2140Strain fitness (log2 ratio) -2 -1 0 1 2at 2137.186 kb on + strand, within Psest_2018at 2137.186 kb on + strand, within Psest_2018at 2137.187 kb on - strand, within Psest_2018at 2137.365 kb on + strand, within Psest_2018at 2137.575 kb on + strand, within Psest_2018at 2137.575 kb on + strand, within Psest_2018at 2137.576 kb on - strand, within Psest_2018at 2137.617 kb on + strand, within Psest_2018at 2137.618 kb on - strand, within Psest_2018at 2138.004 kb on + strandat 2138.005 kb on - strandat 2138.005 kb on - strandat 2138.007 kb on - strandat 2138.008 kb on + strandat 2138.009 kb on - strandat 2138.009 kb on - strandat 2139.483 kb on - strandat 2139.483 kb on - strandat 2139.483 kb on - strandat 2139.754 kb on + strand, within Psest_2020at 2139.754 kb on + strand, within Psest_2020at 2139.754 kb on + strand, within Psest_2020at 2139.756 kb on + strand, within Psest_2020at 2139.756 kb on + strand, within Psest_2020at 2139.757 kb on - strand, within Psest_2020at 2139.905 kb on - strand, within Psest_2020at 2139.910 kb on - strand, within Psest_2020at 2140.000 kb on + strand, within Psest_2020

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Per-strain Table

Position Strand Gene LocusTag Fraction Def Chlorate 20 mM
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2,137,186 + Psest_2018 0.31 -0.2
2,137,186 + Psest_2018 0.31 -0.2
2,137,187 - Psest_2018 0.31 +0.3
2,137,365 + Psest_2018 0.47 +0.7
2,137,575 + Psest_2018 0.65 +1.9
2,137,575 + Psest_2018 0.65 -1.5
2,137,576 - Psest_2018 0.65 -1.3
2,137,617 + Psest_2018 0.68 -1.6
2,137,618 - Psest_2018 0.68 +0.9
2,138,004 + -2.5
2,138,005 - +0.6
2,138,005 - -0.4
2,138,007 - -0.9
2,138,008 + -0.7
2,138,009 - +0.0
2,138,009 - +0.4
2,139,483 - -0.6
2,139,483 - -0.3
2,139,483 - +0.3
2,139,754 + Psest_2020 0.36 -0.1
2,139,754 + Psest_2020 0.36 -2.0
2,139,754 + Psest_2020 0.36 +1.5
2,139,756 + Psest_2020 0.37 -0.3
2,139,756 + Psest_2020 0.37 +0.7
2,139,757 - Psest_2020 0.37 +1.4
2,139,905 - Psest_2020 0.60 +1.5
2,139,910 - Psest_2020 0.61 +0.7
2,140,000 + Psest_2020 0.75 +1.2

Or see this region's nucleotide sequence