Experiment: Def Chlorate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2018 and Psest_2019 are separated by 76 nucleotides Psest_2019 and Psest_2020 are separated by 87 nucleotides Psest_2020 and Psest_2021 overlap by 4 nucleotides
Psest_2018: Psest_2018 - Uncharacterized conserved protein, at 2,136,821 to 2,137,987
_2018
Psest_2019: Psest_2019 - adenylosuccinate lyase, at 2,138,064 to 2,139,434
_2019
Psest_2020: Psest_2020 - Uncharacterized protein involved in purine metabolism, at 2,139,522 to 2,140,160
_2020
Psest_2021: Psest_2021 - tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase, at 2,140,157 to 2,141,284
_2021
Position (kb)
2138
2139
2140 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2137.186 kb on + strand, within Psest_2018 at 2137.186 kb on + strand, within Psest_2018 at 2137.187 kb on - strand, within Psest_2018 at 2137.365 kb on + strand, within Psest_2018 at 2137.575 kb on + strand, within Psest_2018 at 2137.575 kb on + strand, within Psest_2018 at 2137.576 kb on - strand, within Psest_2018 at 2137.617 kb on + strand, within Psest_2018 at 2137.618 kb on - strand, within Psest_2018 at 2138.004 kb on + strand at 2138.005 kb on - strand at 2138.005 kb on - strand at 2138.007 kb on - strand at 2138.008 kb on + strand at 2138.009 kb on - strand at 2138.009 kb on - strand at 2139.483 kb on - strand at 2139.483 kb on - strand at 2139.483 kb on - strand at 2139.754 kb on + strand, within Psest_2020 at 2139.754 kb on + strand, within Psest_2020 at 2139.754 kb on + strand, within Psest_2020 at 2139.756 kb on + strand, within Psest_2020 at 2139.756 kb on + strand, within Psest_2020 at 2139.757 kb on - strand, within Psest_2020 at 2139.905 kb on - strand, within Psest_2020 at 2139.910 kb on - strand, within Psest_2020 at 2140.000 kb on + strand, within Psest_2020
Per-strain Table
Position Strand Gene LocusTag Fraction Def Chlorate 20 mM remove 2,137,186 + Psest_2018 0.31 -0.2 2,137,186 + Psest_2018 0.31 -0.2 2,137,187 - Psest_2018 0.31 +0.3 2,137,365 + Psest_2018 0.47 +0.7 2,137,575 + Psest_2018 0.65 +1.9 2,137,575 + Psest_2018 0.65 -1.5 2,137,576 - Psest_2018 0.65 -1.3 2,137,617 + Psest_2018 0.68 -1.6 2,137,618 - Psest_2018 0.68 +0.9 2,138,004 + -2.5 2,138,005 - +0.6 2,138,005 - -0.4 2,138,007 - -0.9 2,138,008 + -0.7 2,138,009 - +0.0 2,138,009 - +0.4 2,139,483 - -0.6 2,139,483 - -0.3 2,139,483 - +0.3 2,139,754 + Psest_2020 0.36 -0.1 2,139,754 + Psest_2020 0.36 -2.0 2,139,754 + Psest_2020 0.36 +1.5 2,139,756 + Psest_2020 0.37 -0.3 2,139,756 + Psest_2020 0.37 +0.7 2,139,757 - Psest_2020 0.37 +1.4 2,139,905 - Psest_2020 0.60 +1.5 2,139,910 - Psest_2020 0.61 +0.7 2,140,000 + Psest_2020 0.75 +1.2
Or see this region's nucleotide sequence