Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_1986

Experiment: Def Chlorate 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_1985 and Psest_1986 overlap by 4 nucleotidesPsest_1986 and Psest_1987 overlap by 4 nucleotidesPsest_1987 and Psest_1988 are separated by 191 nucleotides Psest_1985: Psest_1985 - histidinol-phosphate aminotransferase, at 2,101,462 to 2,102,571 _1985 Psest_1986: Psest_1986 - 3-phosphoshikimate 1-carboxyvinyltransferase, at 2,102,568 to 2,104,811 _1986 Psest_1987: Psest_1987 - cytidylate kinase, at 2,104,808 to 2,105,497 _1987 Psest_1988: Psest_1988 - ribosomal protein S1, at 2,105,689 to 2,107,371 _1988 Position (kb) 2102 2103 2104 2105Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2101.625 kb on + strand, within Psest_1985at 2101.625 kb on + strand, within Psest_1985at 2102.059 kb on + strand, within Psest_1985at 2102.059 kb on + strand, within Psest_1985at 2102.059 kb on + strand, within Psest_1985at 2102.059 kb on + strand, within Psest_1985at 2102.059 kb on + strand, within Psest_1985at 2102.059 kb on + strand, within Psest_1985at 2102.059 kb on + strand, within Psest_1985at 2102.182 kb on + strand, within Psest_1985at 2102.213 kb on + strand, within Psest_1985at 2102.247 kb on + strand, within Psest_1985at 2102.293 kb on + strand, within Psest_1985at 2103.460 kb on + strand, within Psest_1986at 2103.477 kb on + strand, within Psest_1986

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Per-strain Table

Position Strand Gene LocusTag Fraction Def Chlorate 20 mM
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2,101,625 + Psest_1985 0.15 -1.2
2,101,625 + Psest_1985 0.15 -0.5
2,102,059 + Psest_1985 0.54 -0.7
2,102,059 + Psest_1985 0.54 +0.3
2,102,059 + Psest_1985 0.54 -0.3
2,102,059 + Psest_1985 0.54 -1.2
2,102,059 + Psest_1985 0.54 -3.3
2,102,059 + Psest_1985 0.54 -1.5
2,102,059 + Psest_1985 0.54 -0.6
2,102,182 + Psest_1985 0.65 -0.6
2,102,213 + Psest_1985 0.68 +0.3
2,102,247 + Psest_1985 0.71 +2.6
2,102,293 + Psest_1985 0.75 -0.8
2,103,460 + Psest_1986 0.40 +0.4
2,103,477 + Psest_1986 0.41 -0.2

Or see this region's nucleotide sequence