Experiment: Choline chloride 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3140 and SO3140.1 are separated by 524 nucleotides SO3140.1 and SO3140.2 are separated by 38 nucleotides SO3140.2 and SO3140.3 overlap by 4 nucleotides
SO3140: SO3140 - thymidine kinase (NCBI ptt file), at 3,273,023 to 3,273,601
SO3140
SO3140.1: SO3140.1 - hypothetical protein (NCBI ptt file), at 3,274,126 to 3,274,533
SO3140.1
SO3140.2: SO3140.2 - hypothetical protein (NCBI ptt file), at 3,274,572 to 3,275,504
SO3140.2
SO3140.3: SO3140.3 - hypothetical protein (NCBI ptt file), at 3,275,501 to 3,275,869
SO3140.3
Position (kb)
3274
3275 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3273.143 kb on + strand, within SO3140 at 3273.237 kb on + strand, within SO3140 at 3273.237 kb on + strand, within SO3140 at 3273.266 kb on - strand, within SO3140 at 3273.496 kb on + strand, within SO3140 at 3273.537 kb on + strand, within SO3140 at 3273.646 kb on + strand at 3273.646 kb on + strand at 3273.684 kb on + strand at 3273.692 kb on - strand at 3274.068 kb on + strand at 3274.083 kb on + strand at 3274.127 kb on - strand at 3274.204 kb on + strand, within SO3140.1 at 3274.279 kb on + strand, within SO3140.1 at 3274.294 kb on - strand, within SO3140.1 at 3274.448 kb on - strand, within SO3140.1 at 3274.519 kb on - strand at 3274.522 kb on + strand at 3274.535 kb on + strand at 3274.579 kb on + strand at 3274.587 kb on - strand at 3274.593 kb on + strand at 3274.643 kb on + strand at 3274.651 kb on - strand at 3274.651 kb on - strand at 3274.700 kb on - strand, within SO3140.2 at 3274.708 kb on - strand, within SO3140.2 at 3274.734 kb on - strand, within SO3140.2 at 3274.806 kb on - strand, within SO3140.2 at 3274.823 kb on - strand, within SO3140.2 at 3274.870 kb on + strand, within SO3140.2 at 3275.011 kb on - strand, within SO3140.2 at 3275.081 kb on + strand, within SO3140.2 at 3275.089 kb on - strand, within SO3140.2 at 3275.114 kb on + strand, within SO3140.2 at 3275.232 kb on + strand, within SO3140.2 at 3275.240 kb on - strand, within SO3140.2 at 3275.280 kb on - strand, within SO3140.2 at 3275.289 kb on - strand, within SO3140.2 at 3275.313 kb on + strand, within SO3140.2 at 3275.371 kb on + strand, within SO3140.2 at 3275.379 kb on - strand, within SO3140.2 at 3275.379 kb on - strand, within SO3140.2 at 3275.379 kb on - strand, within SO3140.2 at 3275.379 kb on - strand, within SO3140.2 at 3275.489 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 150 mM remove 3,273,143 + SO3140 0.21 +0.7 3,273,237 + SO3140 0.37 -0.3 3,273,237 + SO3140 0.37 -1.2 3,273,266 - SO3140 0.42 +0.8 3,273,496 + SO3140 0.82 +0.2 3,273,537 + SO3140 0.89 -1.0 3,273,646 + +0.3 3,273,646 + -0.9 3,273,684 + -2.3 3,273,692 - +0.2 3,274,068 + +1.1 3,274,083 + -0.1 3,274,127 - +0.1 3,274,204 + SO3140.1 0.19 -0.4 3,274,279 + SO3140.1 0.38 -1.1 3,274,294 - SO3140.1 0.41 -0.6 3,274,448 - SO3140.1 0.79 -0.5 3,274,519 - +1.1 3,274,522 + -1.2 3,274,535 + +0.2 3,274,579 + -0.4 3,274,587 - -0.2 3,274,593 + -1.1 3,274,643 + +0.3 3,274,651 - +0.9 3,274,651 - -0.0 3,274,700 - SO3140.2 0.14 +0.5 3,274,708 - SO3140.2 0.15 -0.2 3,274,734 - SO3140.2 0.17 +2.4 3,274,806 - SO3140.2 0.25 -0.3 3,274,823 - SO3140.2 0.27 +1.5 3,274,870 + SO3140.2 0.32 -0.4 3,275,011 - SO3140.2 0.47 +0.2 3,275,081 + SO3140.2 0.55 -0.2 3,275,089 - SO3140.2 0.55 -0.4 3,275,114 + SO3140.2 0.58 -0.2 3,275,232 + SO3140.2 0.71 +0.5 3,275,240 - SO3140.2 0.72 -1.0 3,275,280 - SO3140.2 0.76 +0.8 3,275,289 - SO3140.2 0.77 +0.6 3,275,313 + SO3140.2 0.79 -0.3 3,275,371 + SO3140.2 0.86 -0.1 3,275,379 - SO3140.2 0.86 -0.6 3,275,379 - SO3140.2 0.86 -0.7 3,275,379 - SO3140.2 0.86 -0.5 3,275,379 - SO3140.2 0.86 -0.6 3,275,489 - -0.0
Or see this region's nucleotide sequence