Strain Fitness in Shewanella oneidensis MR-1 around SO3670

Experiment: Choline chloride 150 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthugA and tonB1 are separated by 245 nucleotidestonB1 and exbB1 are separated by 56 nucleotidesexbB1 and exbD1 overlap by 4 nucleotides SO3669: hugA - heme transport protein (NCBI ptt file), at 3,821,526 to 3,823,619 hugA SO3670: tonB1 - TonB1 protein (NCBI ptt file), at 3,823,865 to 3,824,689 tonB1 SO3671: exbB1 - TonB system transport protein ExbB1 (NCBI ptt file), at 3,824,746 to 3,825,528 exbB1 SO3672: exbD1 - TonB system transport protein ExbD1 (NCBI ptt file), at 3,825,525 to 3,825,962 exbD1 Position (kb) 3823 3824 3825Strain fitness (log2 ratio) -3 -2 -1 0 1at 3823.002 kb on + strand, within hugAat 3823.011 kb on - strand, within hugAat 3823.044 kb on + strand, within hugAat 3823.136 kb on + strand, within hugAat 3823.144 kb on - strand, within hugAat 3823.144 kb on - strand, within hugAat 3823.144 kb on - strand, within hugAat 3823.196 kb on + strand, within hugAat 3823.280 kb on + strand, within hugAat 3823.412 kb on + strandat 3823.437 kb on - strandat 3823.464 kb on + strandat 3823.464 kb on + strandat 3823.472 kb on - strandat 3823.472 kb on - strandat 3823.472 kb on - strandat 3823.505 kb on - strandat 3823.510 kb on - strandat 3823.520 kb on - strandat 3823.520 kb on - strandat 3823.561 kb on - strandat 3823.742 kb on + strandat 3823.921 kb on + strandat 3823.921 kb on + strandat 3823.929 kb on - strandat 3823.929 kb on - strandat 3823.936 kb on + strandat 3823.944 kb on - strandat 3823.944 kb on - strandat 3823.951 kb on + strand, within tonB1at 3823.983 kb on - strand, within tonB1at 3823.989 kb on - strand, within tonB1at 3823.998 kb on - strand, within tonB1at 3824.012 kb on - strand, within tonB1at 3824.126 kb on + strand, within tonB1at 3824.144 kb on - strand, within tonB1at 3824.185 kb on + strand, within tonB1at 3824.250 kb on + strand, within tonB1at 3824.250 kb on + strand, within tonB1at 3824.258 kb on - strand, within tonB1at 3824.449 kb on + strand, within tonB1at 3824.563 kb on - strand, within tonB1at 3824.575 kb on - strand, within tonB1at 3824.576 kb on - strand, within tonB1at 3824.581 kb on + strand, within tonB1at 3824.631 kb on + strandat 3824.656 kb on + strandat 3824.656 kb on + strandat 3824.705 kb on + strandat 3824.705 kb on + strandat 3824.713 kb on - strandat 3824.715 kb on - strandat 3824.803 kb on - strandat 3824.803 kb on - strandat 3824.831 kb on + strand, within exbB1at 3824.834 kb on - strand, within exbB1at 3824.997 kb on + strand, within exbB1at 3825.035 kb on + strand, within exbB1at 3825.074 kb on - strand, within exbB1at 3825.103 kb on - strand, within exbB1at 3825.103 kb on - strand, within exbB1at 3825.172 kb on + strand, within exbB1at 3825.196 kb on - strand, within exbB1at 3825.229 kb on - strand, within exbB1at 3825.243 kb on + strand, within exbB1at 3825.243 kb on + strand, within exbB1at 3825.243 kb on + strand, within exbB1at 3825.251 kb on - strand, within exbB1at 3825.251 kb on - strand, within exbB1at 3825.251 kb on - strand, within exbB1at 3825.256 kb on - strand, within exbB1at 3825.258 kb on + strand, within exbB1at 3825.304 kb on - strand, within exbB1at 3825.388 kb on - strand, within exbB1at 3825.439 kb on - strand, within exbB1at 3825.441 kb on + strand, within exbB1at 3825.478 kb on + strandat 3825.486 kb on + strandat 3825.527 kb on + strandat 3825.532 kb on + strandat 3825.546 kb on + strandat 3825.554 kb on - strandat 3825.639 kb on + strand, within exbD1

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Per-strain Table

Position Strand Gene LocusTag Fraction Choline chloride 150 mM
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3,823,002 + hugA SO3669 0.70 -0.3
3,823,011 - hugA SO3669 0.71 -0.3
3,823,044 + hugA SO3669 0.72 -0.9
3,823,136 + hugA SO3669 0.77 +0.3
3,823,144 - hugA SO3669 0.77 -0.5
3,823,144 - hugA SO3669 0.77 -0.3
3,823,144 - hugA SO3669 0.77 -0.6
3,823,196 + hugA SO3669 0.80 +0.7
3,823,280 + hugA SO3669 0.84 -1.1
3,823,412 + +0.3
3,823,437 - -0.2
3,823,464 + +0.7
3,823,464 + +0.2
3,823,472 - -0.3
3,823,472 - -0.2
3,823,472 - +0.4
3,823,505 - -0.2
3,823,510 - +1.3
3,823,520 - -0.4
3,823,520 - +0.2
3,823,561 - +0.9
3,823,742 + +0.1
3,823,921 + -1.0
3,823,921 + -0.1
3,823,929 - +0.3
3,823,929 - +0.5
3,823,936 + -0.0
3,823,944 - -0.5
3,823,944 - +0.4
3,823,951 + tonB1 SO3670 0.10 -1.2
3,823,983 - tonB1 SO3670 0.14 -0.8
3,823,989 - tonB1 SO3670 0.15 -1.1
3,823,998 - tonB1 SO3670 0.16 -0.2
3,824,012 - tonB1 SO3670 0.18 -1.6
3,824,126 + tonB1 SO3670 0.32 -0.3
3,824,144 - tonB1 SO3670 0.34 -1.2
3,824,185 + tonB1 SO3670 0.39 -0.4
3,824,250 + tonB1 SO3670 0.47 -2.8
3,824,250 + tonB1 SO3670 0.47 -1.1
3,824,258 - tonB1 SO3670 0.48 -1.0
3,824,449 + tonB1 SO3670 0.71 -0.8
3,824,563 - tonB1 SO3670 0.85 +0.5
3,824,575 - tonB1 SO3670 0.86 +0.4
3,824,576 - tonB1 SO3670 0.86 +0.5
3,824,581 + tonB1 SO3670 0.87 +0.7
3,824,631 + -0.5
3,824,656 + -0.6
3,824,656 + -0.0
3,824,705 + -0.1
3,824,705 + -0.6
3,824,713 - +0.1
3,824,715 - -1.8
3,824,803 - -0.7
3,824,803 - -1.3
3,824,831 + exbB1 SO3671 0.11 +0.7
3,824,834 - exbB1 SO3671 0.11 +0.6
3,824,997 + exbB1 SO3671 0.32 +0.2
3,825,035 + exbB1 SO3671 0.37 +0.5
3,825,074 - exbB1 SO3671 0.42 -0.2
3,825,103 - exbB1 SO3671 0.46 -0.6
3,825,103 - exbB1 SO3671 0.46 -0.5
3,825,172 + exbB1 SO3671 0.54 +0.3
3,825,196 - exbB1 SO3671 0.57 +0.9
3,825,229 - exbB1 SO3671 0.62 -0.1
3,825,243 + exbB1 SO3671 0.63 -0.2
3,825,243 + exbB1 SO3671 0.63 +0.3
3,825,243 + exbB1 SO3671 0.63 -0.1
3,825,251 - exbB1 SO3671 0.64 +0.9
3,825,251 - exbB1 SO3671 0.64 -1.4
3,825,251 - exbB1 SO3671 0.64 +1.1
3,825,256 - exbB1 SO3671 0.65 +0.4
3,825,258 + exbB1 SO3671 0.65 -1.1
3,825,304 - exbB1 SO3671 0.71 -0.6
3,825,388 - exbB1 SO3671 0.82 -0.5
3,825,439 - exbB1 SO3671 0.89 -0.3
3,825,441 + exbB1 SO3671 0.89 +1.0
3,825,478 + -0.2
3,825,486 + +0.2
3,825,527 + +0.8
3,825,532 + -0.7
3,825,546 + -1.0
3,825,554 - -0.3
3,825,639 + exbD1 SO3672 0.26 -0.8

Or see this region's nucleotide sequence