Experiment: Choline chloride 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hugA and tonB1 are separated by 245 nucleotides tonB1 and exbB1 are separated by 56 nucleotides exbB1 and exbD1 overlap by 4 nucleotides
SO3669: hugA - heme transport protein (NCBI ptt file), at 3,821,526 to 3,823,619
hugA
SO3670: tonB1 - TonB1 protein (NCBI ptt file), at 3,823,865 to 3,824,689
tonB1
SO3671: exbB1 - TonB system transport protein ExbB1 (NCBI ptt file), at 3,824,746 to 3,825,528
exbB1
SO3672: exbD1 - TonB system transport protein ExbD1 (NCBI ptt file), at 3,825,525 to 3,825,962
exbD1
Position (kb)
3823
3824
3825 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3823.002 kb on + strand, within hugA at 3823.011 kb on - strand, within hugA at 3823.044 kb on + strand, within hugA at 3823.136 kb on + strand, within hugA at 3823.144 kb on - strand, within hugA at 3823.144 kb on - strand, within hugA at 3823.144 kb on - strand, within hugA at 3823.196 kb on + strand, within hugA at 3823.280 kb on + strand, within hugA at 3823.412 kb on + strand at 3823.437 kb on - strand at 3823.464 kb on + strand at 3823.464 kb on + strand at 3823.472 kb on - strand at 3823.472 kb on - strand at 3823.472 kb on - strand at 3823.505 kb on - strand at 3823.510 kb on - strand at 3823.520 kb on - strand at 3823.520 kb on - strand at 3823.561 kb on - strand at 3823.742 kb on + strand at 3823.921 kb on + strand at 3823.921 kb on + strand at 3823.929 kb on - strand at 3823.929 kb on - strand at 3823.936 kb on + strand at 3823.944 kb on - strand at 3823.944 kb on - strand at 3823.951 kb on + strand, within tonB1 at 3823.983 kb on - strand, within tonB1 at 3823.989 kb on - strand, within tonB1 at 3823.998 kb on - strand, within tonB1 at 3824.012 kb on - strand, within tonB1 at 3824.126 kb on + strand, within tonB1 at 3824.144 kb on - strand, within tonB1 at 3824.185 kb on + strand, within tonB1 at 3824.250 kb on + strand, within tonB1 at 3824.250 kb on + strand, within tonB1 at 3824.258 kb on - strand, within tonB1 at 3824.449 kb on + strand, within tonB1 at 3824.563 kb on - strand, within tonB1 at 3824.575 kb on - strand, within tonB1 at 3824.576 kb on - strand, within tonB1 at 3824.581 kb on + strand, within tonB1 at 3824.631 kb on + strand at 3824.656 kb on + strand at 3824.656 kb on + strand at 3824.705 kb on + strand at 3824.705 kb on + strand at 3824.713 kb on - strand at 3824.715 kb on - strand at 3824.803 kb on - strand at 3824.803 kb on - strand at 3824.831 kb on + strand, within exbB1 at 3824.834 kb on - strand, within exbB1 at 3824.997 kb on + strand, within exbB1 at 3825.035 kb on + strand, within exbB1 at 3825.074 kb on - strand, within exbB1 at 3825.103 kb on - strand, within exbB1 at 3825.103 kb on - strand, within exbB1 at 3825.172 kb on + strand, within exbB1 at 3825.196 kb on - strand, within exbB1 at 3825.229 kb on - strand, within exbB1 at 3825.243 kb on + strand, within exbB1 at 3825.243 kb on + strand, within exbB1 at 3825.243 kb on + strand, within exbB1 at 3825.251 kb on - strand, within exbB1 at 3825.251 kb on - strand, within exbB1 at 3825.251 kb on - strand, within exbB1 at 3825.256 kb on - strand, within exbB1 at 3825.258 kb on + strand, within exbB1 at 3825.304 kb on - strand, within exbB1 at 3825.388 kb on - strand, within exbB1 at 3825.439 kb on - strand, within exbB1 at 3825.441 kb on + strand, within exbB1 at 3825.478 kb on + strand at 3825.486 kb on + strand at 3825.527 kb on + strand at 3825.532 kb on + strand at 3825.546 kb on + strand at 3825.554 kb on - strand at 3825.639 kb on + strand, within exbD1
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 150 mM remove 3,823,002 + hugA SO3669 0.70 -0.3 3,823,011 - hugA SO3669 0.71 -0.3 3,823,044 + hugA SO3669 0.72 -0.9 3,823,136 + hugA SO3669 0.77 +0.3 3,823,144 - hugA SO3669 0.77 -0.5 3,823,144 - hugA SO3669 0.77 -0.3 3,823,144 - hugA SO3669 0.77 -0.6 3,823,196 + hugA SO3669 0.80 +0.7 3,823,280 + hugA SO3669 0.84 -1.1 3,823,412 + +0.3 3,823,437 - -0.2 3,823,464 + +0.7 3,823,464 + +0.2 3,823,472 - -0.3 3,823,472 - -0.2 3,823,472 - +0.4 3,823,505 - -0.2 3,823,510 - +1.3 3,823,520 - -0.4 3,823,520 - +0.2 3,823,561 - +0.9 3,823,742 + +0.1 3,823,921 + -1.0 3,823,921 + -0.1 3,823,929 - +0.3 3,823,929 - +0.5 3,823,936 + -0.0 3,823,944 - -0.5 3,823,944 - +0.4 3,823,951 + tonB1 SO3670 0.10 -1.2 3,823,983 - tonB1 SO3670 0.14 -0.8 3,823,989 - tonB1 SO3670 0.15 -1.1 3,823,998 - tonB1 SO3670 0.16 -0.2 3,824,012 - tonB1 SO3670 0.18 -1.6 3,824,126 + tonB1 SO3670 0.32 -0.3 3,824,144 - tonB1 SO3670 0.34 -1.2 3,824,185 + tonB1 SO3670 0.39 -0.4 3,824,250 + tonB1 SO3670 0.47 -2.8 3,824,250 + tonB1 SO3670 0.47 -1.1 3,824,258 - tonB1 SO3670 0.48 -1.0 3,824,449 + tonB1 SO3670 0.71 -0.8 3,824,563 - tonB1 SO3670 0.85 +0.5 3,824,575 - tonB1 SO3670 0.86 +0.4 3,824,576 - tonB1 SO3670 0.86 +0.5 3,824,581 + tonB1 SO3670 0.87 +0.7 3,824,631 + -0.5 3,824,656 + -0.6 3,824,656 + -0.0 3,824,705 + -0.1 3,824,705 + -0.6 3,824,713 - +0.1 3,824,715 - -1.8 3,824,803 - -0.7 3,824,803 - -1.3 3,824,831 + exbB1 SO3671 0.11 +0.7 3,824,834 - exbB1 SO3671 0.11 +0.6 3,824,997 + exbB1 SO3671 0.32 +0.2 3,825,035 + exbB1 SO3671 0.37 +0.5 3,825,074 - exbB1 SO3671 0.42 -0.2 3,825,103 - exbB1 SO3671 0.46 -0.6 3,825,103 - exbB1 SO3671 0.46 -0.5 3,825,172 + exbB1 SO3671 0.54 +0.3 3,825,196 - exbB1 SO3671 0.57 +0.9 3,825,229 - exbB1 SO3671 0.62 -0.1 3,825,243 + exbB1 SO3671 0.63 -0.2 3,825,243 + exbB1 SO3671 0.63 +0.3 3,825,243 + exbB1 SO3671 0.63 -0.1 3,825,251 - exbB1 SO3671 0.64 +0.9 3,825,251 - exbB1 SO3671 0.64 -1.4 3,825,251 - exbB1 SO3671 0.64 +1.1 3,825,256 - exbB1 SO3671 0.65 +0.4 3,825,258 + exbB1 SO3671 0.65 -1.1 3,825,304 - exbB1 SO3671 0.71 -0.6 3,825,388 - exbB1 SO3671 0.82 -0.5 3,825,439 - exbB1 SO3671 0.89 -0.3 3,825,441 + exbB1 SO3671 0.89 +1.0 3,825,478 + -0.2 3,825,486 + +0.2 3,825,527 + +0.8 3,825,532 + -0.7 3,825,546 + -1.0 3,825,554 - -0.3 3,825,639 + exbD1 SO3672 0.26 -0.8
Or see this region's nucleotide sequence