Strain Fitness in Shewanella oneidensis MR-1 around SO3414

Experiment: L-Glutamic (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthrC and thrB are separated by 72 nucleotidesthrB and thrA overlap by 4 nucleotides SO3413: thrC - threonine synthase (NCBI ptt file), at 3,552,916 to 3,554,199 thrC SO3414: thrB - homoserine kinase (NCBI ptt file), at 3,554,272 to 3,555,210 thrB SO3415: thrA - aspartokinase I/homoserine dehydrogenase, threonine-sensitive (NCBI ptt file), at 3,555,207 to 3,557,675 thrA Position (kb) 3554 3555 3556Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 3553.294 kb on + strand, within thrCat 3553.312 kb on + strand, within thrCat 3553.331 kb on + strand, within thrCat 3553.339 kb on - strand, within thrCat 3553.339 kb on - strand, within thrCat 3553.385 kb on - strand, within thrCat 3553.385 kb on - strand, within thrCat 3553.503 kb on + strand, within thrCat 3553.594 kb on + strand, within thrCat 3553.733 kb on + strand, within thrCat 3553.733 kb on + strand, within thrCat 3553.783 kb on - strand, within thrCat 3553.788 kb on - strand, within thrCat 3553.959 kb on - strand, within thrCat 3554.007 kb on + strand, within thrCat 3554.007 kb on + strand, within thrCat 3554.007 kb on + strand, within thrCat 3554.007 kb on + strand, within thrCat 3554.015 kb on - strand, within thrCat 3554.015 kb on - strand, within thrCat 3554.015 kb on - strand, within thrCat 3554.015 kb on - strand, within thrCat 3554.051 kb on + strand, within thrCat 3554.103 kb on - strandat 3554.122 kb on + strandat 3554.151 kb on + strandat 3554.221 kb on - strandat 3554.224 kb on + strandat 3554.232 kb on - strandat 3554.244 kb on + strandat 3554.279 kb on + strandat 3554.279 kb on + strandat 3554.279 kb on + strandat 3554.481 kb on - strand, within thrBat 3554.498 kb on + strand, within thrBat 3554.505 kb on - strand, within thrBat 3554.556 kb on + strand, within thrBat 3554.612 kb on - strand, within thrBat 3554.612 kb on - strand, within thrBat 3554.656 kb on + strand, within thrBat 3554.689 kb on - strand, within thrBat 3554.689 kb on - strand, within thrBat 3554.694 kb on + strand, within thrBat 3554.694 kb on + strand, within thrBat 3554.784 kb on + strand, within thrBat 3554.795 kb on - strand, within thrBat 3554.892 kb on + strand, within thrBat 3554.911 kb on + strand, within thrBat 3554.918 kb on - strand, within thrBat 3554.955 kb on - strand, within thrBat 3555.075 kb on + strand, within thrBat 3555.092 kb on + strand, within thrBat 3555.206 kb on + strandat 3555.206 kb on + strandat 3555.218 kb on + strandat 3555.224 kb on - strandat 3555.280 kb on - strandat 3555.295 kb on - strandat 3555.305 kb on - strandat 3555.310 kb on - strandat 3555.398 kb on - strandat 3555.439 kb on - strandat 3555.448 kb on + strandat 3555.448 kb on + strandat 3555.456 kb on - strand, within thrAat 3555.456 kb on - strand, within thrAat 3555.638 kb on + strand, within thrAat 3555.726 kb on - strand, within thrAat 3555.780 kb on - strand, within thrAat 3555.878 kb on + strand, within thrAat 3556.011 kb on - strand, within thrAat 3556.027 kb on + strand, within thrAat 3556.058 kb on + strand, within thrA

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamic (N)
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3,553,294 + thrC SO3413 0.29 -3.2
3,553,312 + thrC SO3413 0.31 -5.5
3,553,331 + thrC SO3413 0.32 -1.8
3,553,339 - thrC SO3413 0.33 -2.1
3,553,339 - thrC SO3413 0.33 -1.6
3,553,385 - thrC SO3413 0.37 -1.5
3,553,385 - thrC SO3413 0.37 -1.5
3,553,503 + thrC SO3413 0.46 -1.8
3,553,594 + thrC SO3413 0.53 -2.6
3,553,733 + thrC SO3413 0.64 -2.5
3,553,733 + thrC SO3413 0.64 -1.8
3,553,783 - thrC SO3413 0.68 -3.4
3,553,788 - thrC SO3413 0.68 -3.1
3,553,959 - thrC SO3413 0.81 -2.4
3,554,007 + thrC SO3413 0.85 -4.9
3,554,007 + thrC SO3413 0.85 -2.6
3,554,007 + thrC SO3413 0.85 -2.4
3,554,007 + thrC SO3413 0.85 -1.9
3,554,015 - thrC SO3413 0.86 -4.2
3,554,015 - thrC SO3413 0.86 -3.3
3,554,015 - thrC SO3413 0.86 -2.5
3,554,015 - thrC SO3413 0.86 -3.2
3,554,051 + thrC SO3413 0.88 -4.6
3,554,103 - -3.2
3,554,122 + -3.4
3,554,151 + -3.3
3,554,221 - -2.4
3,554,224 + -3.4
3,554,232 - -1.4
3,554,244 + -2.0
3,554,279 + -4.0
3,554,279 + -2.6
3,554,279 + -2.0
3,554,481 - thrB SO3414 0.22 -2.5
3,554,498 + thrB SO3414 0.24 -3.1
3,554,505 - thrB SO3414 0.25 -3.4
3,554,556 + thrB SO3414 0.30 -0.9
3,554,612 - thrB SO3414 0.36 -2.0
3,554,612 - thrB SO3414 0.36 -3.1
3,554,656 + thrB SO3414 0.41 -3.3
3,554,689 - thrB SO3414 0.44 -3.7
3,554,689 - thrB SO3414 0.44 -3.0
3,554,694 + thrB SO3414 0.45 -4.4
3,554,694 + thrB SO3414 0.45 -5.0
3,554,784 + thrB SO3414 0.55 +1.2
3,554,795 - thrB SO3414 0.56 -4.2
3,554,892 + thrB SO3414 0.66 -4.5
3,554,911 + thrB SO3414 0.68 -4.2
3,554,918 - thrB SO3414 0.69 -2.2
3,554,955 - thrB SO3414 0.73 -3.1
3,555,075 + thrB SO3414 0.86 -3.0
3,555,092 + thrB SO3414 0.87 -4.6
3,555,206 + -3.2
3,555,206 + -3.2
3,555,218 + -2.6
3,555,224 - -1.4
3,555,280 - -0.7
3,555,295 - +0.8
3,555,305 - -0.4
3,555,310 - -0.5
3,555,398 - -0.3
3,555,439 - -0.3
3,555,448 + -2.6
3,555,448 + -3.3
3,555,456 - thrA SO3415 0.10 +0.2
3,555,456 - thrA SO3415 0.10 +0.3
3,555,638 + thrA SO3415 0.17 -2.5
3,555,726 - thrA SO3415 0.21 -0.7
3,555,780 - thrA SO3415 0.23 -0.2
3,555,878 + thrA SO3415 0.27 -2.7
3,556,011 - thrA SO3415 0.33 -1.1
3,556,027 + thrA SO3415 0.33 -3.9
3,556,058 + thrA SO3415 0.34 -2.6

Or see this region's nucleotide sequence