Strain Fitness in Shewanella oneidensis MR-1 around SO3211

Experiment: L-Glutamic (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcheY-3 and fliA are separated by 47 nucleotidesfliA and flhG overlap by 8 nucleotidesflhG and flhF are separated by 34 nucleotides SO3209: cheY-3 - chemotaxis protein CheY (NCBI ptt file), at 3,349,724 to 3,350,107 cheY-3 SO3210: fliA - RNA polymerase sigma-27 factor (NCBI ptt file), at 3,350,155 to 3,350,868 fliA SO3211: flhG - flagellar biosynthetic protein FlhG (NCBI ptt file), at 3,350,861 to 3,351,712 flhG SO3212: flhF - flagellar biosynthetic protein FlhF (NCBI ptt file), at 3,351,747 to 3,353,123 flhF Position (kb) 3350 3351 3352Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3349.899 kb on + strand, within cheY-3at 3350.007 kb on + strand, within cheY-3at 3350.116 kb on - strandat 3350.191 kb on - strandat 3350.534 kb on - strand, within fliAat 3350.671 kb on - strand, within fliAat 3350.686 kb on - strand, within fliAat 3350.731 kb on - strand, within fliAat 3350.732 kb on + strand, within fliAat 3350.845 kb on + strandat 3350.853 kb on - strandat 3350.853 kb on - strandat 3350.853 kb on - strandat 3351.311 kb on - strand, within flhGat 3351.457 kb on - strand, within flhGat 3351.511 kb on - strand, within flhGat 3351.571 kb on - strand, within flhGat 3351.591 kb on + strand, within flhGat 3351.638 kb on + strandat 3351.646 kb on - strandat 3351.770 kb on - strandat 3351.770 kb on - strandat 3351.982 kb on - strand, within flhFat 3352.025 kb on - strand, within flhFat 3352.032 kb on + strand, within flhFat 3352.045 kb on - strand, within flhFat 3352.109 kb on + strand, within flhFat 3352.260 kb on + strand, within flhFat 3352.310 kb on - strand, within flhFat 3352.383 kb on + strand, within flhFat 3352.621 kb on - strand, within flhFat 3352.690 kb on + strand, within flhF

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamic (N)
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3,349,899 + cheY-3 SO3209 0.46 +1.5
3,350,007 + cheY-3 SO3209 0.74 +2.1
3,350,116 - +1.0
3,350,191 - +1.9
3,350,534 - fliA SO3210 0.53 +2.3
3,350,671 - fliA SO3210 0.72 +1.8
3,350,686 - fliA SO3210 0.74 +2.2
3,350,731 - fliA SO3210 0.81 +2.4
3,350,732 + fliA SO3210 0.81 +3.1
3,350,845 + +2.0
3,350,853 - +1.4
3,350,853 - +2.8
3,350,853 - +1.6
3,351,311 - flhG SO3211 0.53 -3.5
3,351,457 - flhG SO3211 0.70 -3.2
3,351,511 - flhG SO3211 0.76 -0.9
3,351,571 - flhG SO3211 0.83 -2.9
3,351,591 + flhG SO3211 0.86 -0.4
3,351,638 + +0.1
3,351,646 - -2.7
3,351,770 - -0.5
3,351,770 - +0.6
3,351,982 - flhF SO3212 0.17 +0.3
3,352,025 - flhF SO3212 0.20 +0.1
3,352,032 + flhF SO3212 0.21 -1.4
3,352,045 - flhF SO3212 0.22 -0.4
3,352,109 + flhF SO3212 0.26 -1.3
3,352,260 + flhF SO3212 0.37 +1.9
3,352,310 - flhF SO3212 0.41 -0.4
3,352,383 + flhF SO3212 0.46 -0.6
3,352,621 - flhF SO3212 0.63 +0.3
3,352,690 + flhF SO3212 0.68 -0.4

Or see this region's nucleotide sequence