Experiment: L-Glutamic (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3063 and purF are separated by 95 nucleotides purF and SO3065 are separated by 182 nucleotides SO3065 and SO3066 are separated by 109 nucleotides
SO3063: SO3063 - sodium:alanine symporter family protein (NCBI ptt file), at 3,178,629 to 3,180,116
SO3063
SO3064: purF - amidophosphoribosyltransferase (NCBI ptt file), at 3,180,212 to 3,181,726
purF
SO3065: SO3065 - colicin V production protein (NCBI ptt file), at 3,181,909 to 3,182,397
SO3065
SO3066: SO3066 - conserved hypothetical protein (NCBI ptt file), at 3,182,507 to 3,183,352
SO3066
Position (kb)
3180
3181
3182 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3179.261 kb on + strand, within SO3063 at 3179.430 kb on + strand, within SO3063 at 3179.430 kb on + strand, within SO3063 at 3179.438 kb on - strand, within SO3063 at 3179.466 kb on + strand, within SO3063 at 3179.547 kb on + strand, within SO3063 at 3179.580 kb on + strand, within SO3063 at 3179.587 kb on + strand, within SO3063 at 3179.595 kb on - strand, within SO3063 at 3179.665 kb on + strand, within SO3063 at 3179.690 kb on - strand, within SO3063 at 3180.065 kb on + strand at 3180.065 kb on + strand at 3180.203 kb on + strand at 3180.236 kb on - strand at 3180.242 kb on - strand at 3180.293 kb on - strand at 3180.343 kb on - strand at 3180.669 kb on + strand, within purF at 3180.886 kb on - strand, within purF at 3180.897 kb on - strand, within purF at 3181.235 kb on - strand, within purF at 3181.268 kb on + strand, within purF at 3181.330 kb on + strand, within purF at 3181.407 kb on - strand, within purF at 3181.554 kb on - strand, within purF at 3181.587 kb on + strand at 3181.595 kb on - strand at 3181.645 kb on - strand at 3181.652 kb on - strand at 3182.017 kb on + strand, within SO3065 at 3182.025 kb on - strand, within SO3065 at 3182.059 kb on + strand, within SO3065 at 3182.420 kb on + strand at 3182.428 kb on - strand at 3182.448 kb on - strand at 3182.590 kb on - strand at 3182.638 kb on + strand, within SO3066 at 3182.658 kb on + strand, within SO3066 at 3182.658 kb on + strand, within SO3066 at 3182.658 kb on + strand, within SO3066 at 3182.666 kb on - strand, within SO3066
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamic (N) remove 3,179,261 + SO3063 0.42 -1.3 3,179,430 + SO3063 0.54 +0.1 3,179,430 + SO3063 0.54 -0.3 3,179,438 - SO3063 0.54 +0.1 3,179,466 + SO3063 0.56 +0.6 3,179,547 + SO3063 0.62 -0.5 3,179,580 + SO3063 0.64 -0.6 3,179,587 + SO3063 0.64 +1.0 3,179,595 - SO3063 0.65 +1.3 3,179,665 + SO3063 0.70 +0.5 3,179,690 - SO3063 0.71 -0.3 3,180,065 + +0.2 3,180,065 + -0.7 3,180,203 + -1.5 3,180,236 - -0.5 3,180,242 - +0.8 3,180,293 - -0.1 3,180,343 - -2.1 3,180,669 + purF SO3064 0.30 -3.2 3,180,886 - purF SO3064 0.44 -0.6 3,180,897 - purF SO3064 0.45 -1.4 3,181,235 - purF SO3064 0.68 -2.5 3,181,268 + purF SO3064 0.70 -2.0 3,181,330 + purF SO3064 0.74 -2.4 3,181,407 - purF SO3064 0.79 -0.8 3,181,554 - purF SO3064 0.89 -0.5 3,181,587 + -1.8 3,181,595 - -2.3 3,181,645 - -2.2 3,181,652 - -1.6 3,182,017 + SO3065 0.22 -1.9 3,182,025 - SO3065 0.24 +0.2 3,182,059 + SO3065 0.31 -1.4 3,182,420 + -2.4 3,182,428 - +2.3 3,182,448 - -0.7 3,182,590 - -0.2 3,182,638 + SO3066 0.15 -1.5 3,182,658 + SO3066 0.18 +0.0 3,182,658 + SO3066 0.18 -0.8 3,182,658 + SO3066 0.18 -0.2 3,182,666 - SO3066 0.19 -0.7
Or see this region's nucleotide sequence