Strain Fitness in Shewanella oneidensis MR-1 around SO3022

Experiment: L-Glutamic (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttrpD and trpC/F are separated by 92 nucleotidestrpC/F and trpB are separated by 119 nucleotides SO3021: trpD - anthranilate phosphoribosyltransferase (NCBI ptt file), at 3,132,201 to 3,133,244 trpD SO3022: trpC/F - indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase (NCBI ptt file), at 3,133,337 to 3,134,746 trpC/F SO3023: trpB - tryptophan synthase, beta subunit (NCBI ptt file), at 3,134,866 to 3,136,056 trpB Position (kb) 3133 3134 3135Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3132.412 kb on - strand, within trpDat 3132.418 kb on - strand, within trpDat 3132.419 kb on - strand, within trpDat 3132.504 kb on + strand, within trpDat 3132.596 kb on - strand, within trpDat 3132.625 kb on + strand, within trpDat 3132.625 kb on + strand, within trpDat 3132.646 kb on + strand, within trpDat 3132.646 kb on + strand, within trpDat 3132.646 kb on + strand, within trpDat 3132.654 kb on - strand, within trpDat 3132.654 kb on - strand, within trpDat 3132.681 kb on + strand, within trpDat 3132.711 kb on + strand, within trpDat 3132.775 kb on - strand, within trpDat 3132.801 kb on + strand, within trpDat 3132.809 kb on - strand, within trpDat 3132.811 kb on + strand, within trpDat 3132.843 kb on + strand, within trpDat 3132.873 kb on + strand, within trpDat 3132.932 kb on + strand, within trpDat 3133.085 kb on - strand, within trpDat 3133.174 kb on - strandat 3133.229 kb on - strandat 3133.230 kb on - strandat 3133.268 kb on + strandat 3133.337 kb on + strandat 3133.337 kb on + strandat 3133.345 kb on - strandat 3133.406 kb on + strandat 3133.407 kb on + strandat 3133.415 kb on - strandat 3133.540 kb on - strand, within trpC/Fat 3133.540 kb on - strand, within trpC/Fat 3133.659 kb on + strand, within trpC/Fat 3133.766 kb on + strand, within trpC/Fat 3133.945 kb on + strand, within trpC/Fat 3134.058 kb on + strand, within trpC/Fat 3134.139 kb on + strand, within trpC/Fat 3134.249 kb on + strand, within trpC/Fat 3134.249 kb on + strand, within trpC/Fat 3134.257 kb on - strand, within trpC/Fat 3134.345 kb on + strand, within trpC/Fat 3134.365 kb on - strand, within trpC/Fat 3134.365 kb on - strand, within trpC/Fat 3134.399 kb on - strand, within trpC/Fat 3134.402 kb on + strand, within trpC/Fat 3134.431 kb on - strand, within trpC/Fat 3134.454 kb on + strand, within trpC/Fat 3134.454 kb on + strand, within trpC/Fat 3134.496 kb on - strand, within trpC/Fat 3134.552 kb on - strand, within trpC/Fat 3134.703 kb on + strandat 3134.797 kb on + strandat 3134.850 kb on + strandat 3134.880 kb on - strandat 3134.996 kb on - strand, within trpBat 3134.996 kb on - strand, within trpBat 3135.032 kb on + strand, within trpBat 3135.111 kb on - strand, within trpBat 3135.139 kb on + strand, within trpBat 3135.139 kb on + strand, within trpBat 3135.205 kb on + strand, within trpBat 3135.205 kb on + strand, within trpBat 3135.213 kb on - strand, within trpBat 3135.226 kb on + strand, within trpBat 3135.226 kb on + strand, within trpBat 3135.234 kb on - strand, within trpBat 3135.262 kb on + strand, within trpBat 3135.262 kb on + strand, within trpBat 3135.262 kb on + strand, within trpBat 3135.264 kb on + strand, within trpBat 3135.270 kb on - strand, within trpBat 3135.270 kb on - strand, within trpBat 3135.270 kb on - strand, within trpBat 3135.270 kb on - strand, within trpBat 3135.272 kb on - strand, within trpBat 3135.272 kb on - strand, within trpBat 3135.398 kb on + strand, within trpBat 3135.624 kb on + strand, within trpBat 3135.651 kb on - strand, within trpBat 3135.659 kb on + strand, within trpB

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamic (N)
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3,132,412 - trpD SO3021 0.20 -3.4
3,132,418 - trpD SO3021 0.21 -3.0
3,132,419 - trpD SO3021 0.21 -2.6
3,132,504 + trpD SO3021 0.29 -3.2
3,132,596 - trpD SO3021 0.38 -1.7
3,132,625 + trpD SO3021 0.41 -3.3
3,132,625 + trpD SO3021 0.41 -3.2
3,132,646 + trpD SO3021 0.43 -1.4
3,132,646 + trpD SO3021 0.43 -3.6
3,132,646 + trpD SO3021 0.43 -0.5
3,132,654 - trpD SO3021 0.43 -1.1
3,132,654 - trpD SO3021 0.43 -2.5
3,132,681 + trpD SO3021 0.46 -2.5
3,132,711 + trpD SO3021 0.49 -2.0
3,132,775 - trpD SO3021 0.55 -1.3
3,132,801 + trpD SO3021 0.57 -2.4
3,132,809 - trpD SO3021 0.58 -2.1
3,132,811 + trpD SO3021 0.58 -3.0
3,132,843 + trpD SO3021 0.61 -4.1
3,132,873 + trpD SO3021 0.64 -1.8
3,132,932 + trpD SO3021 0.70 -1.8
3,133,085 - trpD SO3021 0.85 -3.9
3,133,174 - -4.6
3,133,229 - -2.5
3,133,230 - -4.0
3,133,268 + -1.1
3,133,337 + -4.0
3,133,337 + -2.2
3,133,345 - -1.3
3,133,406 + -2.9
3,133,407 + -3.1
3,133,415 - -2.0
3,133,540 - trpC/F SO3022 0.14 -2.9
3,133,540 - trpC/F SO3022 0.14 -1.5
3,133,659 + trpC/F SO3022 0.23 -2.1
3,133,766 + trpC/F SO3022 0.30 -2.7
3,133,945 + trpC/F SO3022 0.43 -2.6
3,134,058 + trpC/F SO3022 0.51 -3.0
3,134,139 + trpC/F SO3022 0.57 -2.1
3,134,249 + trpC/F SO3022 0.65 -1.5
3,134,249 + trpC/F SO3022 0.65 -2.3
3,134,257 - trpC/F SO3022 0.65 -2.5
3,134,345 + trpC/F SO3022 0.71 -3.2
3,134,365 - trpC/F SO3022 0.73 -3.3
3,134,365 - trpC/F SO3022 0.73 -4.3
3,134,399 - trpC/F SO3022 0.75 -4.4
3,134,402 + trpC/F SO3022 0.76 -2.2
3,134,431 - trpC/F SO3022 0.78 -1.3
3,134,454 + trpC/F SO3022 0.79 -0.9
3,134,454 + trpC/F SO3022 0.79 -2.8
3,134,496 - trpC/F SO3022 0.82 -3.0
3,134,552 - trpC/F SO3022 0.86 -1.9
3,134,703 + -1.4
3,134,797 + -0.4
3,134,850 + -2.3
3,134,880 - -3.7
3,134,996 - trpB SO3023 0.11 -2.3
3,134,996 - trpB SO3023 0.11 -2.9
3,135,032 + trpB SO3023 0.14 -3.2
3,135,111 - trpB SO3023 0.21 -2.5
3,135,139 + trpB SO3023 0.23 -2.3
3,135,139 + trpB SO3023 0.23 -1.9
3,135,205 + trpB SO3023 0.28 -4.2
3,135,205 + trpB SO3023 0.28 -2.6
3,135,213 - trpB SO3023 0.29 -3.2
3,135,226 + trpB SO3023 0.30 -3.5
3,135,226 + trpB SO3023 0.30 -0.3
3,135,234 - trpB SO3023 0.31 -1.0
3,135,262 + trpB SO3023 0.33 -3.1
3,135,262 + trpB SO3023 0.33 -1.9
3,135,262 + trpB SO3023 0.33 -2.8
3,135,264 + trpB SO3023 0.33 -3.0
3,135,270 - trpB SO3023 0.34 -3.6
3,135,270 - trpB SO3023 0.34 -1.3
3,135,270 - trpB SO3023 0.34 -1.9
3,135,270 - trpB SO3023 0.34 -2.3
3,135,272 - trpB SO3023 0.34 -2.7
3,135,272 - trpB SO3023 0.34 -2.2
3,135,398 + trpB SO3023 0.45 -4.2
3,135,624 + trpB SO3023 0.64 -3.4
3,135,651 - trpB SO3023 0.66 -3.3
3,135,659 + trpB SO3023 0.67 -4.6

Or see this region's nucleotide sequence