Strain Fitness in Shewanella oneidensis MR-1 around SO2067

Experiment: L-Glutamic (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttyrP and hisI are separated by 579 nucleotideshisI and hisF are separated by 82 nucleotideshisF and hisA overlap by 19 nucleotides SO2065: tyrP - tyrosine-specific transport protein (NCBI ptt file), at 2,162,328 to 2,163,515 tyrP SO2067: hisI - phosphoribosyl-ATP pyrophosphatase/phosphoribosyl-AMP cyclohydrolase (NCBI ptt file), at 2,164,095 to 2,164,730 hisI SO2068: hisF - hisF protein (cyclase) (NCBI ptt file), at 2,164,813 to 2,165,586 hisF SO2069: hisA - phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (NCBI ptt file), at 2,165,568 to 2,166,305 hisA Position (kb) 2164 2165Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 2163.101 kb on - strand, within tyrPat 2163.101 kb on - strand, within tyrPat 2163.354 kb on + strand, within tyrPat 2163.359 kb on + strand, within tyrPat 2163.424 kb on - strandat 2163.424 kb on - strandat 2163.458 kb on + strandat 2163.630 kb on + strandat 2163.657 kb on + strandat 2163.703 kb on - strandat 2163.876 kb on - strandat 2164.152 kb on + strandat 2164.153 kb on - strandat 2164.207 kb on + strand, within hisIat 2164.318 kb on + strand, within hisIat 2164.511 kb on + strand, within hisIat 2164.600 kb on + strand, within hisIat 2164.800 kb on + strandat 2164.800 kb on + strandat 2164.851 kb on + strandat 2164.917 kb on - strand, within hisFat 2164.924 kb on + strand, within hisFat 2164.953 kb on + strand, within hisFat 2165.133 kb on - strand, within hisFat 2165.306 kb on - strand, within hisFat 2165.328 kb on - strand, within hisFat 2165.390 kb on + strand, within hisFat 2165.398 kb on - strand, within hisFat 2165.421 kb on - strand, within hisFat 2165.528 kb on + strandat 2165.529 kb on - strandat 2165.561 kb on - strandat 2165.583 kb on + strandat 2165.602 kb on + strandat 2165.660 kb on + strand, within hisAat 2165.695 kb on - strand, within hisA

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamic (N)
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2,163,101 - tyrP SO2065 0.65 +0.5
2,163,101 - tyrP SO2065 0.65 +1.0
2,163,354 + tyrP SO2065 0.86 +0.6
2,163,359 + tyrP SO2065 0.87 +0.3
2,163,424 - +0.1
2,163,424 - -0.4
2,163,458 + +0.1
2,163,630 + +0.2
2,163,657 + +0.1
2,163,703 - +1.0
2,163,876 - +1.0
2,164,152 + -3.4
2,164,153 - -0.5
2,164,207 + hisI SO2067 0.18 -2.8
2,164,318 + hisI SO2067 0.35 -1.8
2,164,511 + hisI SO2067 0.65 -1.1
2,164,600 + hisI SO2067 0.79 -2.5
2,164,800 + -2.2
2,164,800 + -2.0
2,164,851 + -4.0
2,164,917 - hisF SO2068 0.13 -5.1
2,164,924 + hisF SO2068 0.14 -3.0
2,164,953 + hisF SO2068 0.18 -1.9
2,165,133 - hisF SO2068 0.41 -3.4
2,165,306 - hisF SO2068 0.64 -1.8
2,165,328 - hisF SO2068 0.67 -4.3
2,165,390 + hisF SO2068 0.75 -5.1
2,165,398 - hisF SO2068 0.76 -2.8
2,165,421 - hisF SO2068 0.79 -1.5
2,165,528 + -3.0
2,165,529 - -4.1
2,165,561 - -1.0
2,165,583 + -2.1
2,165,602 + -2.4
2,165,660 + hisA SO2069 0.12 -2.9
2,165,695 - hisA SO2069 0.17 -1.4

Or see this region's nucleotide sequence