Experiment: L-Glutamic (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt folD and tRNA-Pro-1 are separated by 354 nucleotides tRNA-Pro-1 and tRNA-Arg-1 are separated by 90 nucleotides tRNA-Arg-1 and tRNA-His-1 are separated by 9 nucleotides tRNA-His-1 and tig are separated by 261 nucleotides tig and clpP are separated by 89 nucleotides clpP and clpX are separated by 99 nucleotides
SO1792: folD - methylenetetrahydrofolate dehydrogenase/methylenetetrahydrofolate cyclohydrolase (NCBI ptt file), at 1,878,808 to 1,879,662
folD
SO_t024: tRNA-Pro-1 - tRNA-Pro (RefSeq), at 1,880,017 to 1,880,093
tRNA-Pro-1
SO_t025: tRNA-Arg-1 - tRNA-Arg (RefSeq), at 1,880,184 to 1,880,260
tRNA-Arg-1
SO_t026: tRNA-His-1 - tRNA-His (RefSeq), at 1,880,270 to 1,880,345
tRNA-His-1
SO1793: tig - trigger factor (NCBI ptt file), at 1,880,607 to 1,881,911
tig
SO1794: clpP - ATP-dependent Clp protease, proteolytic subunit (NCBI ptt file), at 1,882,001 to 1,882,609
clpP
SO1795: clpX - ATP-dependent Clp protease, ATP-binding subunit ClpX (NCBI ptt file), at 1,882,709 to 1,883,989
clpX
Position (kb)
1880
1881
1882 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1879.665 kb on - strand at 1880.454 kb on - strand at 1880.783 kb on - strand, within tig at 1881.009 kb on + strand, within tig at 1881.017 kb on - strand, within tig at 1881.017 kb on - strand, within tig at 1881.062 kb on + strand, within tig at 1881.117 kb on + strand, within tig at 1881.371 kb on - strand, within tig at 1881.597 kb on - strand, within tig at 1881.836 kb on + strand at 1881.836 kb on + strand at 1881.900 kb on - strand at 1882.028 kb on + strand at 1882.278 kb on + strand, within clpP at 1882.285 kb on + strand, within clpP at 1882.423 kb on - strand, within clpP at 1882.594 kb on + strand at 1882.625 kb on + strand at 1882.633 kb on - strand at 1882.666 kb on + strand at 1882.819 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamic (N) remove 1,879,665 - +1.2 1,880,454 - +0.4 1,880,783 - tig SO1793 0.13 -1.9 1,881,009 + tig SO1793 0.31 -1.5 1,881,017 - tig SO1793 0.31 -1.7 1,881,017 - tig SO1793 0.31 -1.7 1,881,062 + tig SO1793 0.35 -2.1 1,881,117 + tig SO1793 0.39 -4.0 1,881,371 - tig SO1793 0.59 +0.3 1,881,597 - tig SO1793 0.76 -3.2 1,881,836 + -1.0 1,881,836 + -1.2 1,881,900 - -1.2 1,882,028 + -2.3 1,882,278 + clpP SO1794 0.45 -4.1 1,882,285 + clpP SO1794 0.47 -1.8 1,882,423 - clpP SO1794 0.69 -1.9 1,882,594 + -1.0 1,882,625 + -0.5 1,882,633 - -2.2 1,882,666 + +1.0 1,882,819 - -0.6
Or see this region's nucleotide sequence