Strain Fitness in Shewanella oneidensis MR-1 around SO1531

Experiment: L-Glutamic (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpomB and thiI are separated by 199 nucleotidesthiI and SO1532 are separated by 132 nucleotidesSO1532 and SO1533 are separated by 176 nucleotides SO1530: pomB - sodium-driven polar flagellar protein PomB (NCBI ptt file), at 1,605,282 to 1,606,208 pomB SO1531: thiI - thiamine biosynthesis protein ThiI (NCBI ptt file), at 1,606,408 to 1,607,862 thiI SO1532: SO1532 - hypothetical protein (NCBI ptt file), at 1,607,995 to 1,608,228 SO1532 SO1533: SO1533 - glycine cleavage system transcriptional activator, putative (NCBI ptt file), at 1,608,405 to 1,609,316 SO1533 Position (kb) 1606 1607 1608Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1605.414 kb on - strand, within pomBat 1605.569 kb on + strand, within pomBat 1605.579 kb on + strand, within pomBat 1605.614 kb on + strand, within pomBat 1605.622 kb on - strand, within pomBat 1605.933 kb on - strand, within pomBat 1606.045 kb on - strand, within pomBat 1606.233 kb on - strandat 1606.293 kb on + strandat 1606.293 kb on + strandat 1606.301 kb on - strandat 1606.321 kb on - strandat 1606.457 kb on - strandat 1606.457 kb on - strandat 1606.496 kb on + strandat 1606.575 kb on + strand, within thiIat 1606.596 kb on + strand, within thiIat 1606.642 kb on + strand, within thiIat 1606.642 kb on + strand, within thiIat 1606.651 kb on + strand, within thiIat 1606.798 kb on - strand, within thiIat 1606.813 kb on - strand, within thiIat 1606.837 kb on + strand, within thiIat 1606.898 kb on - strand, within thiIat 1606.915 kb on + strand, within thiIat 1606.980 kb on - strand, within thiIat 1606.984 kb on + strand, within thiIat 1607.193 kb on - strand, within thiIat 1607.225 kb on + strand, within thiIat 1607.302 kb on + strand, within thiIat 1607.320 kb on - strand, within thiIat 1607.320 kb on - strand, within thiIat 1607.321 kb on + strand, within thiIat 1607.411 kb on - strand, within thiIat 1607.427 kb on + strand, within thiIat 1607.435 kb on - strand, within thiIat 1607.484 kb on + strand, within thiIat 1607.484 kb on + strand, within thiIat 1607.492 kb on - strand, within thiIat 1607.498 kb on - strand, within thiIat 1607.688 kb on + strand, within thiIat 1607.796 kb on - strandat 1607.870 kb on + strandat 1607.914 kb on + strandat 1607.967 kb on + strandat 1608.030 kb on - strand, within SO1532at 1608.126 kb on - strand, within SO1532at 1608.154 kb on + strand, within SO1532at 1608.242 kb on - strandat 1608.242 kb on - strandat 1608.286 kb on - strandat 1608.506 kb on + strand, within SO1533at 1608.663 kb on + strand, within SO1533at 1608.859 kb on + strand, within SO1533at 1608.859 kb on + strand, within SO1533at 1608.859 kb on + strand, within SO1533

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamic (N)
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1,605,414 - pomB SO1530 0.14 +1.1
1,605,569 + pomB SO1530 0.31 +2.2
1,605,579 + pomB SO1530 0.32 +0.7
1,605,614 + pomB SO1530 0.36 +1.1
1,605,622 - pomB SO1530 0.37 +0.9
1,605,933 - pomB SO1530 0.70 +1.4
1,606,045 - pomB SO1530 0.82 +0.8
1,606,233 - +1.5
1,606,293 + -0.2
1,606,293 + -0.4
1,606,301 - -1.1
1,606,321 - +1.3
1,606,457 - -1.9
1,606,457 - -0.8
1,606,496 + +0.1
1,606,575 + thiI SO1531 0.11 -1.1
1,606,596 + thiI SO1531 0.13 -1.9
1,606,642 + thiI SO1531 0.16 -1.7
1,606,642 + thiI SO1531 0.16 -3.1
1,606,651 + thiI SO1531 0.17 -2.2
1,606,798 - thiI SO1531 0.27 -1.8
1,606,813 - thiI SO1531 0.28 -1.9
1,606,837 + thiI SO1531 0.29 -1.4
1,606,898 - thiI SO1531 0.34 -0.9
1,606,915 + thiI SO1531 0.35 -1.5
1,606,980 - thiI SO1531 0.39 -2.0
1,606,984 + thiI SO1531 0.40 -0.7
1,607,193 - thiI SO1531 0.54 -1.5
1,607,225 + thiI SO1531 0.56 -1.1
1,607,302 + thiI SO1531 0.61 -1.3
1,607,320 - thiI SO1531 0.63 -0.9
1,607,320 - thiI SO1531 0.63 -0.6
1,607,321 + thiI SO1531 0.63 -2.1
1,607,411 - thiI SO1531 0.69 -1.1
1,607,427 + thiI SO1531 0.70 -1.2
1,607,435 - thiI SO1531 0.71 -0.4
1,607,484 + thiI SO1531 0.74 -2.5
1,607,484 + thiI SO1531 0.74 -0.6
1,607,492 - thiI SO1531 0.75 -1.4
1,607,498 - thiI SO1531 0.75 -2.3
1,607,688 + thiI SO1531 0.88 -1.1
1,607,796 - -2.2
1,607,870 + -0.2
1,607,914 + -0.1
1,607,967 + -0.0
1,608,030 - SO1532 0.15 -0.7
1,608,126 - SO1532 0.56 -0.5
1,608,154 + SO1532 0.68 -0.7
1,608,242 - -0.2
1,608,242 - -0.9
1,608,286 - +1.3
1,608,506 + SO1533 0.11 +1.0
1,608,663 + SO1533 0.28 -0.2
1,608,859 + SO1533 0.50 -0.3
1,608,859 + SO1533 0.50 +0.1
1,608,859 + SO1533 0.50 +0.2

Or see this region's nucleotide sequence