Experiment: L-Glutamic (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt petB and petC overlap by 1 nucleotides petC and sspA are separated by 96 nucleotides sspA and sspB overlap by 1 nucleotides sspB and pabA are separated by 154 nucleotides
SO0609: petB - ubiquinol-cytochrome c reductase, cytochrome b (NCBI ptt file), at 634,389 to 635,603
petB
SO0610: petC - ubiquinol-cytochrome c reductase, cytochrome c1 (NCBI ptt file), at 635,603 to 636,298
petC
SO0611: sspA - stringent starvation protein a (NCBI ptt file), at 636,395 to 637,024
sspA
SO0612: sspB - stringent starvation protein b (NCBI ptt file), at 637,024 to 637,461
sspB
SO0613: pabA - para-aminobenzoate synthase glutamine amidotransferase, component II (NCBI ptt file), at 637,616 to 638,191
pabA
Position (kb) 636
637
638 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 635.701 kb on + strand, within petC at 636.333 kb on + strand at 636.346 kb on + strand at 636.500 kb on - strand, within sspA at 636.584 kb on - strand, within sspA at 636.717 kb on - strand, within sspA at 636.727 kb on - strand, within sspA at 636.845 kb on + strand, within sspA at 636.878 kb on + strand, within sspA at 636.878 kb on + strand, within sspA at 636.898 kb on + strand, within sspA at 636.934 kb on - strand, within sspA at 637.064 kb on - strand at 637.587 kb on + strand at 637.587 kb on + strand at 637.587 kb on + strand at 637.587 kb on + strand at 637.587 kb on + strand at 637.595 kb on - strand at 637.659 kb on - strand at 637.737 kb on + strand, within pabA at 637.742 kb on + strand, within pabA at 637.772 kb on - strand, within pabA at 637.857 kb on - strand, within pabA at 638.009 kb on + strand, within pabA
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamic (N) remove 635,701 + petC SO0610 0.14 +0.5 636,333 + +0.4 636,346 + -1.0 636,500 - sspA SO0611 0.17 -1.0 636,584 - sspA SO0611 0.30 -3.3 636,717 - sspA SO0611 0.51 -4.3 636,727 - sspA SO0611 0.53 -0.4 636,845 + sspA SO0611 0.71 -1.6 636,878 + sspA SO0611 0.77 -2.3 636,878 + sspA SO0611 0.77 -1.6 636,898 + sspA SO0611 0.80 -1.8 636,934 - sspA SO0611 0.86 -3.5 637,064 - +0.7 637,587 + -0.4 637,587 + +0.7 637,587 + +0.2 637,587 + -0.7 637,587 + -0.9 637,595 - +0.6 637,659 - -0.3 637,737 + pabA SO0613 0.21 +0.3 637,742 + pabA SO0613 0.22 +2.0 637,772 - pabA SO0613 0.27 +0.2 637,857 - pabA SO0613 0.42 +0.9 638,009 + pabA SO0613 0.68 +0.4
Or see this region's nucleotide sequence