Experiment: L-Histidine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3199 and SO3200 are separated by 220 nucleotides SO3200 and cheW-3 are separated by 145 nucleotides cheW-3 and SO3203 are separated by 7 nucleotides SO3203 and SO3204 overlap by 17 nucleotides
SO3199: SO3199 - FlhB domain protein (NCBI ptt file), at 3,341,611 to 3,341,919
SO3199
SO3200: SO3200 - conserved hypothetical protein (NCBI ptt file), at 3,342,140 to 3,342,538
SO3200
SO3202: cheW-3 - purine-binding chemotaxis protein CheW (NCBI ptt file), at 3,342,684 to 3,343,178
cheW-3
SO3203: SO3203 - CheW domain protein (NCBI ptt file), at 3,343,186 to 3,344,166
SO3203
SO3204: SO3204 - ParA family protein (NCBI ptt file), at 3,344,150 to 3,344,941
SO3204
Position (kb)
3342
3343
3344 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3341.745 kb on - strand, within SO3199 at 3341.821 kb on - strand, within SO3199 at 3341.948 kb on + strand at 3341.956 kb on - strand at 3341.961 kb on - strand at 3342.004 kb on + strand at 3342.012 kb on - strand at 3342.045 kb on - strand at 3342.089 kb on - strand at 3342.118 kb on + strand at 3342.126 kb on - strand at 3342.177 kb on + strand at 3342.222 kb on - strand, within SO3200 at 3342.506 kb on + strand at 3342.514 kb on - strand at 3342.564 kb on + strand at 3342.603 kb on - strand at 3342.666 kb on + strand at 3342.698 kb on + strand at 3342.698 kb on + strand at 3342.743 kb on + strand, within cheW-3 at 3342.769 kb on + strand, within cheW-3 at 3342.797 kb on + strand, within cheW-3 at 3342.805 kb on - strand, within cheW-3 at 3342.844 kb on - strand, within cheW-3 at 3342.952 kb on + strand, within cheW-3 at 3343.002 kb on + strand, within cheW-3 at 3343.045 kb on + strand, within cheW-3 at 3343.050 kb on + strand, within cheW-3 at 3343.111 kb on - strand, within cheW-3 at 3343.111 kb on - strand, within cheW-3 at 3343.161 kb on - strand at 3343.215 kb on - strand at 3343.561 kb on - strand, within SO3203 at 3343.594 kb on + strand, within SO3203 at 3343.750 kb on - strand, within SO3203 at 3344.090 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (N) remove 3,341,745 - SO3199 0.43 -0.9 3,341,821 - SO3199 0.68 -1.7 3,341,948 + -0.2 3,341,956 - +0.5 3,341,961 - -0.5 3,342,004 + +0.1 3,342,012 - -0.8 3,342,045 - +2.1 3,342,089 - -1.1 3,342,118 + +0.8 3,342,126 - +0.2 3,342,177 + +0.6 3,342,222 - SO3200 0.21 +0.1 3,342,506 + +0.1 3,342,514 - +0.4 3,342,564 + -0.6 3,342,603 - +0.5 3,342,666 + +1.4 3,342,698 + +0.3 3,342,698 + -0.3 3,342,743 + cheW-3 SO3202 0.12 -0.0 3,342,769 + cheW-3 SO3202 0.17 +3.1 3,342,797 + cheW-3 SO3202 0.23 +0.9 3,342,805 - cheW-3 SO3202 0.24 +1.2 3,342,844 - cheW-3 SO3202 0.32 +1.6 3,342,952 + cheW-3 SO3202 0.54 +2.9 3,343,002 + cheW-3 SO3202 0.64 -1.3 3,343,045 + cheW-3 SO3202 0.73 +3.3 3,343,050 + cheW-3 SO3202 0.74 -0.1 3,343,111 - cheW-3 SO3202 0.86 -0.1 3,343,111 - cheW-3 SO3202 0.86 +2.4 3,343,161 - +1.4 3,343,215 - -0.1 3,343,561 - SO3203 0.38 +0.2 3,343,594 + SO3203 0.42 -0.8 3,343,750 - SO3203 0.57 +0.3 3,344,090 + -0.6
Or see this region's nucleotide sequence