Experiment: L-Histidine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ubiG and SO2414 are separated by 1 nucleotides SO2414 and nrdA are separated by 451 nucleotides
SO2413: ubiG - 3-demethylubiquinone-9 3-methyltransferase (NCBI ptt file), at 2,519,024 to 2,519,734
ubiG
SO2414: SO2414 - phosphoglycolate phosphatase, putative (NCBI ptt file), at 2,519,736 to 2,520,425
SO2414
SO2415: nrdA - ribonucleoside-diphosphate reductase, alpha subunit (NCBI ptt file), at 2,520,877 to 2,523,165
nrdA
Position (kb)
2519
2520
2521 Strain fitness (log2 ratio)
-1
0
1
2 at 2518.852 kb on + strand at 2518.944 kb on + strand at 2519.786 kb on + strand at 2519.855 kb on + strand, within SO2414 at 2519.858 kb on - strand, within SO2414 at 2519.888 kb on - strand, within SO2414 at 2519.941 kb on + strand, within SO2414 at 2519.957 kb on - strand, within SO2414 at 2520.039 kb on + strand, within SO2414 at 2520.039 kb on + strand, within SO2414 at 2520.047 kb on - strand, within SO2414 at 2520.092 kb on - strand, within SO2414 at 2520.117 kb on - strand, within SO2414 at 2520.117 kb on - strand, within SO2414 at 2520.215 kb on - strand, within SO2414 at 2520.222 kb on + strand, within SO2414 at 2520.359 kb on + strand at 2520.366 kb on - strand at 2520.384 kb on + strand at 2520.384 kb on + strand at 2520.384 kb on + strand at 2520.443 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (N) remove 2,518,852 + +0.7 2,518,944 + +2.0 2,519,786 + +0.8 2,519,855 + SO2414 0.17 -0.5 2,519,858 - SO2414 0.18 +0.4 2,519,888 - SO2414 0.22 +0.1 2,519,941 + SO2414 0.30 +0.7 2,519,957 - SO2414 0.32 +1.1 2,520,039 + SO2414 0.44 -0.6 2,520,039 + SO2414 0.44 +0.7 2,520,047 - SO2414 0.45 +1.2 2,520,092 - SO2414 0.52 +0.9 2,520,117 - SO2414 0.55 +0.3 2,520,117 - SO2414 0.55 +0.6 2,520,215 - SO2414 0.69 +0.7 2,520,222 + SO2414 0.70 +0.8 2,520,359 + +0.1 2,520,366 - +0.6 2,520,384 + +0.3 2,520,384 + +1.3 2,520,384 + +1.1 2,520,443 + -0.1
Or see this region's nucleotide sequence