Strain Fitness in Shewanella oneidensis MR-1 around SO2414

Experiment: L-Histidine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntubiG and SO2414 are separated by 1 nucleotidesSO2414 and nrdA are separated by 451 nucleotides SO2413: ubiG - 3-demethylubiquinone-9 3-methyltransferase (NCBI ptt file), at 2,519,024 to 2,519,734 ubiG SO2414: SO2414 - phosphoglycolate phosphatase, putative (NCBI ptt file), at 2,519,736 to 2,520,425 SO2414 SO2415: nrdA - ribonucleoside-diphosphate reductase, alpha subunit (NCBI ptt file), at 2,520,877 to 2,523,165 nrdA Position (kb) 2519 2520 2521Strain fitness (log2 ratio) -1 0 1 2at 2518.852 kb on + strandat 2518.944 kb on + strandat 2519.786 kb on + strandat 2519.855 kb on + strand, within SO2414at 2519.858 kb on - strand, within SO2414at 2519.888 kb on - strand, within SO2414at 2519.941 kb on + strand, within SO2414at 2519.957 kb on - strand, within SO2414at 2520.039 kb on + strand, within SO2414at 2520.039 kb on + strand, within SO2414at 2520.047 kb on - strand, within SO2414at 2520.092 kb on - strand, within SO2414at 2520.117 kb on - strand, within SO2414at 2520.117 kb on - strand, within SO2414at 2520.215 kb on - strand, within SO2414at 2520.222 kb on + strand, within SO2414at 2520.359 kb on + strandat 2520.366 kb on - strandat 2520.384 kb on + strandat 2520.384 kb on + strandat 2520.384 kb on + strandat 2520.443 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Histidine (N)
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2,518,852 + +0.7
2,518,944 + +2.0
2,519,786 + +0.8
2,519,855 + SO2414 0.17 -0.5
2,519,858 - SO2414 0.18 +0.4
2,519,888 - SO2414 0.22 +0.1
2,519,941 + SO2414 0.30 +0.7
2,519,957 - SO2414 0.32 +1.1
2,520,039 + SO2414 0.44 -0.6
2,520,039 + SO2414 0.44 +0.7
2,520,047 - SO2414 0.45 +1.2
2,520,092 - SO2414 0.52 +0.9
2,520,117 - SO2414 0.55 +0.3
2,520,117 - SO2414 0.55 +0.6
2,520,215 - SO2414 0.69 +0.7
2,520,222 + SO2414 0.70 +0.8
2,520,359 + +0.1
2,520,366 - +0.6
2,520,384 + +0.3
2,520,384 + +1.3
2,520,384 + +1.1
2,520,443 + -0.1

Or see this region's nucleotide sequence