Experiment: L-Histidine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2359 and SO2360 are separated by 22 nucleotides SO2360 and ccoP are separated by 110 nucleotides ccoP and ccoQ overlap by 1 nucleotides ccoQ and ccoO are separated by 11 nucleotides ccoO and ccoN are separated by 12 nucleotides
SO2359: SO2359 - cation transport ATPase, E1-E2 family (NCBI ptt file), at 2,465,253 to 2,467,652
SO2359
SO2360: SO2360 - conserved hypothetical protein (NCBI ptt file), at 2,467,675 to 2,468,154
SO2360
SO2361: ccoP - cytochrome c oxidase, cbb3-type, subunit III (NCBI ptt file), at 2,468,265 to 2,469,233
ccoP
SO2362: ccoQ - cytochrome c oxidase, cbb3-type, CcoQ subunit (NCBI ptt file), at 2,469,233 to 2,469,409
ccoQ
SO2363: ccoO - cytochrome c oxidase, cbb3-type, subunit II (NCBI ptt file), at 2,469,421 to 2,470,047
ccoO
SO2364: ccoN - cytochrome c oxidase, cbb3-type, subunit I (NCBI ptt file), at 2,470,060 to 2,471,496
ccoN
Position (kb)
2468
2469
2470 Strain fitness (log2 ratio)
-2
-1
0
1 at 2467.267 kb on - strand, within SO2359 at 2467.381 kb on + strand, within SO2359 at 2467.497 kb on - strand at 2467.771 kb on + strand, within SO2360 at 2468.020 kb on + strand, within SO2360 at 2468.135 kb on - strand at 2468.504 kb on - strand, within ccoP at 2468.934 kb on + strand, within ccoP at 2468.953 kb on - strand, within ccoP at 2468.965 kb on - strand, within ccoP at 2468.965 kb on - strand, within ccoP at 2468.994 kb on + strand, within ccoP at 2469.002 kb on - strand, within ccoP at 2469.051 kb on - strand, within ccoP at 2469.089 kb on + strand, within ccoP at 2469.097 kb on - strand, within ccoP at 2469.538 kb on - strand, within ccoO at 2469.618 kb on + strand, within ccoO at 2470.187 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (N) remove 2,467,267 - SO2359 0.84 -2.0 2,467,381 + SO2359 0.89 -0.5 2,467,497 - -1.5 2,467,771 + SO2360 0.20 +0.5 2,468,020 + SO2360 0.72 +0.4 2,468,135 - +0.3 2,468,504 - ccoP SO2361 0.25 -1.8 2,468,934 + ccoP SO2361 0.69 -0.1 2,468,953 - ccoP SO2361 0.71 -0.8 2,468,965 - ccoP SO2361 0.72 -1.3 2,468,965 - ccoP SO2361 0.72 -1.3 2,468,994 + ccoP SO2361 0.75 -1.2 2,469,002 - ccoP SO2361 0.76 +1.1 2,469,051 - ccoP SO2361 0.81 -0.4 2,469,089 + ccoP SO2361 0.85 -0.5 2,469,097 - ccoP SO2361 0.86 -0.2 2,469,538 - ccoO SO2363 0.19 -1.3 2,469,618 + ccoO SO2363 0.31 -1.9 2,470,187 - -1.7
Or see this region's nucleotide sequence