Experiment: L-Histidine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hepA and SO0576 are separated by 388 nucleotides SO0576 and SO0577 are separated by 411 nucleotides
SO0575: hepA - RNA polymerase-associated protein HepA (NCBI ptt file), at 594,280 to 597,186
hepA
SO0576: SO0576 - PhoH family protein (NCBI ptt file), at 597,575 to 598,969
SO0576
SO0577: SO0577 - sensory box histidine kinase/response regulator (NCBI ptt file), at 599,381 to 602,947
SO0577
Position (kb) 597
598
599 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 596.689 kb on - strand, within hepA at 596.697 kb on + strand, within hepA at 596.778 kb on - strand, within hepA at 596.991 kb on - strand at 597.067 kb on + strand at 597.075 kb on - strand at 597.075 kb on - strand at 597.075 kb on - strand at 597.093 kb on - strand at 597.106 kb on + strand at 597.114 kb on - strand at 597.161 kb on - strand at 597.176 kb on - strand at 597.176 kb on - strand at 597.210 kb on + strand at 597.214 kb on + strand at 597.285 kb on - strand at 597.370 kb on + strand at 597.370 kb on + strand at 597.370 kb on + strand at 597.378 kb on - strand at 597.380 kb on + strand at 597.380 kb on + strand at 597.388 kb on - strand at 597.396 kb on - strand at 597.421 kb on - strand at 597.562 kb on + strand at 597.570 kb on - strand at 597.570 kb on - strand at 597.573 kb on + strand at 597.581 kb on - strand at 597.693 kb on + strand at 597.701 kb on - strand at 597.947 kb on + strand, within SO0576 at 597.975 kb on + strand, within SO0576 at 597.980 kb on - strand, within SO0576 at 598.012 kb on + strand, within SO0576 at 598.014 kb on - strand, within SO0576 at 598.020 kb on - strand, within SO0576 at 598.113 kb on + strand, within SO0576 at 598.143 kb on - strand, within SO0576 at 598.147 kb on + strand, within SO0576 at 598.596 kb on + strand, within SO0576 at 598.612 kb on - strand, within SO0576 at 598.736 kb on + strand, within SO0576 at 598.744 kb on + strand, within SO0576 at 598.788 kb on - strand, within SO0576 at 598.859 kb on + strand at 598.862 kb on - strand at 598.867 kb on - strand at 598.902 kb on + strand at 598.910 kb on - strand at 598.966 kb on + strand at 598.969 kb on - strand at 598.980 kb on + strand at 598.996 kb on + strand at 599.182 kb on - strand at 599.409 kb on - strand at 599.625 kb on - strand at 599.625 kb on - strand at 599.625 kb on - strand at 599.693 kb on - strand at 599.722 kb on + strand at 599.893 kb on - strand, within SO0577 at 599.922 kb on + strand, within SO0577 at 599.922 kb on + strand, within SO0577 at 599.930 kb on - strand, within SO0577
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (N) remove 596,689 - hepA SO0575 0.83 -1.9 596,697 + hepA SO0575 0.83 +0.6 596,778 - hepA SO0575 0.86 -0.6 596,991 - -0.1 597,067 + -0.3 597,075 - -1.0 597,075 - +0.2 597,075 - +0.1 597,093 - -0.8 597,106 + +0.9 597,114 - -0.9 597,161 - -0.9 597,176 - +0.1 597,176 - -0.8 597,210 + -0.1 597,214 + -1.0 597,285 - +2.6 597,370 + -0.0 597,370 + +0.2 597,370 + +0.2 597,378 - -0.9 597,380 + +0.7 597,380 + -0.7 597,388 - +0.2 597,396 - +0.3 597,421 - +0.1 597,562 + +0.7 597,570 - +1.2 597,570 - -0.2 597,573 + -1.3 597,581 - -1.2 597,693 + -1.1 597,701 - -0.9 597,947 + SO0576 0.27 -2.5 597,975 + SO0576 0.29 +0.9 597,980 - SO0576 0.29 -0.3 598,012 + SO0576 0.31 -0.4 598,014 - SO0576 0.31 +0.0 598,020 - SO0576 0.32 +0.0 598,113 + SO0576 0.39 -0.2 598,143 - SO0576 0.41 -0.1 598,147 + SO0576 0.41 -0.7 598,596 + SO0576 0.73 -0.0 598,612 - SO0576 0.74 -0.4 598,736 + SO0576 0.83 -0.8 598,744 + SO0576 0.84 -1.0 598,788 - SO0576 0.87 -1.6 598,859 + +0.2 598,862 - -0.2 598,867 - -1.2 598,902 + -1.0 598,910 - +0.4 598,966 + -1.1 598,969 - -0.8 598,980 + -0.5 598,996 + -1.0 599,182 - +2.6 599,409 - +3.7 599,625 - +3.0 599,625 - +3.3 599,625 - +4.2 599,693 - +3.7 599,722 + +1.9 599,893 - SO0577 0.14 +3.8 599,922 + SO0577 0.15 +3.5 599,922 + SO0577 0.15 +4.0 599,930 - SO0577 0.15 +3.4
Or see this region's nucleotide sequence