Experiment: L-Histidine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fdnG and fdnH are separated by 2 nucleotides fdnH and fdnI overlap by 4 nucleotides fdnI and fdhE overlap by 1 nucleotides
SO0101: fdnG - selenium-containing formate dehydrogenase, nitrate inducible, alpha subunit (NCBI ptt file), at 108,703 to 111,717
fdnG
SO0102: fdnH - formate dehydrogenase, nitrate-inducible, iron-sulfur subunit (NCBI ptt file), at 111,720 to 112,634
fdnH
SO0103: fdnI - formate dehydrogenase, nitrate-inducible, cytochrome b556 subunit (NCBI ptt file), at 112,631 to 113,272
fdnI
SO0104: fdhE - fdhE protein (NCBI ptt file), at 113,272 to 114,180
fdhE
Position (kb) 111
112
113 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 110.757 kb on + strand, within fdnG at 110.764 kb on - strand, within fdnG at 110.764 kb on - strand, within fdnG at 110.764 kb on - strand, within fdnG at 110.764 kb on - strand, within fdnG at 110.790 kb on - strand, within fdnG at 110.849 kb on + strand, within fdnG at 110.872 kb on - strand, within fdnG at 110.937 kb on + strand, within fdnG at 110.945 kb on - strand, within fdnG at 110.982 kb on - strand, within fdnG at 110.994 kb on + strand, within fdnG at 111.000 kb on - strand, within fdnG at 111.002 kb on - strand, within fdnG at 111.027 kb on + strand, within fdnG at 111.032 kb on + strand, within fdnG at 111.050 kb on - strand, within fdnG at 111.080 kb on + strand, within fdnG at 111.088 kb on - strand, within fdnG at 111.088 kb on - strand, within fdnG at 111.088 kb on - strand, within fdnG at 111.088 kb on - strand, within fdnG at 111.145 kb on - strand, within fdnG at 111.191 kb on - strand, within fdnG at 111.191 kb on - strand, within fdnG at 111.262 kb on + strand, within fdnG at 111.264 kb on + strand, within fdnG at 111.264 kb on + strand, within fdnG at 111.264 kb on + strand, within fdnG at 111.264 kb on + strand, within fdnG at 111.269 kb on + strand, within fdnG at 111.269 kb on + strand, within fdnG at 111.269 kb on + strand, within fdnG at 111.269 kb on + strand, within fdnG at 111.272 kb on - strand, within fdnG at 111.272 kb on - strand, within fdnG at 111.272 kb on - strand, within fdnG at 111.272 kb on - strand, within fdnG at 111.277 kb on - strand, within fdnG at 111.277 kb on - strand, within fdnG at 111.277 kb on - strand, within fdnG at 111.277 kb on - strand, within fdnG at 111.277 kb on - strand, within fdnG at 111.277 kb on - strand, within fdnG at 111.277 kb on - strand, within fdnG at 111.277 kb on - strand, within fdnG at 111.277 kb on - strand, within fdnG at 111.328 kb on + strand, within fdnG at 111.330 kb on - strand, within fdnG at 111.336 kb on - strand, within fdnG at 111.409 kb on + strand, within fdnG at 111.409 kb on + strand, within fdnG at 111.522 kb on + strand at 111.536 kb on + strand at 111.675 kb on + strand at 111.675 kb on - strand at 111.711 kb on + strand at 111.756 kb on + strand at 111.764 kb on - strand at 111.787 kb on - strand at 111.823 kb on + strand, within fdnH at 111.841 kb on + strand, within fdnH at 111.871 kb on + strand, within fdnH at 111.871 kb on + strand, within fdnH at 111.871 kb on + strand, within fdnH at 111.941 kb on + strand, within fdnH at 112.014 kb on + strand, within fdnH at 112.022 kb on - strand, within fdnH at 112.022 kb on - strand, within fdnH at 112.163 kb on + strand, within fdnH at 112.185 kb on + strand, within fdnH at 112.212 kb on - strand, within fdnH at 112.247 kb on + strand, within fdnH at 112.247 kb on + strand, within fdnH at 112.250 kb on - strand, within fdnH at 112.293 kb on + strand, within fdnH at 112.355 kb on + strand, within fdnH at 112.355 kb on + strand, within fdnH at 112.368 kb on + strand, within fdnH at 112.368 kb on + strand, within fdnH at 112.375 kb on - strand, within fdnH at 112.423 kb on - strand, within fdnH at 112.423 kb on - strand, within fdnH at 112.467 kb on - strand, within fdnH at 112.521 kb on + strand, within fdnH at 112.597 kb on + strand at 112.598 kb on + strand at 112.665 kb on + strand at 112.665 kb on + strand at 112.688 kb on + strand at 112.825 kb on + strand, within fdnI at 112.831 kb on - strand, within fdnI at 112.839 kb on - strand, within fdnI at 112.990 kb on - strand, within fdnI at 112.990 kb on - strand, within fdnI at 113.024 kb on - strand, within fdnI at 113.060 kb on - strand, within fdnI at 113.078 kb on + strand, within fdnI at 113.086 kb on - strand, within fdnI at 113.086 kb on - strand at 113.165 kb on + strand, within fdnI at 113.165 kb on + strand, within fdnI at 113.171 kb on - strand, within fdnI at 113.200 kb on + strand, within fdnI at 113.299 kb on + strand at 113.332 kb on + strand at 113.340 kb on - strand at 113.349 kb on + strand at 113.349 kb on + strand at 113.349 kb on + strand at 113.349 kb on + strand at 113.349 kb on + strand at 113.357 kb on - strand at 113.357 kb on - strand at 113.357 kb on - strand at 113.364 kb on + strand, within fdhE at 113.428 kb on - strand, within fdhE at 113.428 kb on - strand, within fdhE at 113.472 kb on + strand, within fdhE at 113.583 kb on - strand, within fdhE at 113.608 kb on + strand, within fdhE
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (N) remove 110,757 + fdnG SO0101 0.68 -0.9 110,764 - fdnG SO0101 0.68 +0.7 110,764 - fdnG SO0101 0.68 +1.8 110,764 - fdnG SO0101 0.68 +0.7 110,764 - fdnG SO0101 0.68 +1.5 110,790 - fdnG SO0101 0.69 +0.1 110,849 + fdnG SO0101 0.71 -0.1 110,872 - fdnG SO0101 0.72 -0.6 110,937 + fdnG SO0101 0.74 +0.4 110,945 - fdnG SO0101 0.74 -1.1 110,982 - fdnG SO0101 0.76 -0.2 110,994 + fdnG SO0101 0.76 +1.1 111,000 - fdnG SO0101 0.76 -0.2 111,002 - fdnG SO0101 0.76 +1.5 111,027 + fdnG SO0101 0.77 -1.3 111,032 + fdnG SO0101 0.77 +0.0 111,050 - fdnG SO0101 0.78 +0.6 111,080 + fdnG SO0101 0.79 -0.3 111,088 - fdnG SO0101 0.79 +1.2 111,088 - fdnG SO0101 0.79 -0.5 111,088 - fdnG SO0101 0.79 +0.2 111,088 - fdnG SO0101 0.79 +0.4 111,145 - fdnG SO0101 0.81 +0.3 111,191 - fdnG SO0101 0.83 +0.4 111,191 - fdnG SO0101 0.83 +0.1 111,262 + fdnG SO0101 0.85 +0.5 111,264 + fdnG SO0101 0.85 -0.1 111,264 + fdnG SO0101 0.85 -0.7 111,264 + fdnG SO0101 0.85 +0.7 111,264 + fdnG SO0101 0.85 -0.8 111,269 + fdnG SO0101 0.85 +0.7 111,269 + fdnG SO0101 0.85 +0.2 111,269 + fdnG SO0101 0.85 -0.7 111,269 + fdnG SO0101 0.85 +0.4 111,272 - fdnG SO0101 0.85 -0.8 111,272 - fdnG SO0101 0.85 +0.9 111,272 - fdnG SO0101 0.85 +2.8 111,272 - fdnG SO0101 0.85 +0.1 111,277 - fdnG SO0101 0.85 -0.5 111,277 - fdnG SO0101 0.85 +0.1 111,277 - fdnG SO0101 0.85 +2.2 111,277 - fdnG SO0101 0.85 -0.0 111,277 - fdnG SO0101 0.85 -0.7 111,277 - fdnG SO0101 0.85 -2.0 111,277 - fdnG SO0101 0.85 -0.3 111,277 - fdnG SO0101 0.85 +0.8 111,277 - fdnG SO0101 0.85 -1.1 111,328 + fdnG SO0101 0.87 +0.3 111,330 - fdnG SO0101 0.87 +2.4 111,336 - fdnG SO0101 0.87 -0.2 111,409 + fdnG SO0101 0.90 +1.4 111,409 + fdnG SO0101 0.90 -0.9 111,522 + +0.6 111,536 + -0.2 111,675 + -0.5 111,675 - -1.3 111,711 + -1.1 111,756 + -1.2 111,764 - +0.4 111,787 - -1.0 111,823 + fdnH SO0102 0.11 -0.3 111,841 + fdnH SO0102 0.13 -0.6 111,871 + fdnH SO0102 0.17 -0.8 111,871 + fdnH SO0102 0.17 -0.4 111,871 + fdnH SO0102 0.17 +0.6 111,941 + fdnH SO0102 0.24 -1.1 112,014 + fdnH SO0102 0.32 +0.7 112,022 - fdnH SO0102 0.33 -0.7 112,022 - fdnH SO0102 0.33 +1.0 112,163 + fdnH SO0102 0.48 +0.8 112,185 + fdnH SO0102 0.51 -0.1 112,212 - fdnH SO0102 0.54 +0.8 112,247 + fdnH SO0102 0.58 -1.3 112,247 + fdnH SO0102 0.58 +0.2 112,250 - fdnH SO0102 0.58 -0.4 112,293 + fdnH SO0102 0.63 +2.7 112,355 + fdnH SO0102 0.69 -0.1 112,355 + fdnH SO0102 0.69 -0.6 112,368 + fdnH SO0102 0.71 +0.0 112,368 + fdnH SO0102 0.71 -1.3 112,375 - fdnH SO0102 0.72 -0.2 112,423 - fdnH SO0102 0.77 +0.2 112,423 - fdnH SO0102 0.77 -0.5 112,467 - fdnH SO0102 0.82 -1.7 112,521 + fdnH SO0102 0.88 -0.3 112,597 + +0.2 112,598 + -0.5 112,665 + -1.1 112,665 + -0.6 112,688 + +0.5 112,825 + fdnI SO0103 0.30 +0.4 112,831 - fdnI SO0103 0.31 +0.6 112,839 - fdnI SO0103 0.32 +1.8 112,990 - fdnI SO0103 0.56 +0.4 112,990 - fdnI SO0103 0.56 -1.0 113,024 - fdnI SO0103 0.61 -0.3 113,060 - fdnI SO0103 0.67 +1.3 113,078 + fdnI SO0103 0.70 +0.0 113,086 - fdnI SO0103 0.71 -1.7 113,086 - +2.3 113,165 + fdnI SO0103 0.83 +0.4 113,165 + fdnI SO0103 0.83 +0.8 113,171 - fdnI SO0103 0.84 -0.4 113,200 + fdnI SO0103 0.89 -0.1 113,299 + +0.8 113,332 + +1.3 113,340 - +1.2 113,349 + +0.4 113,349 + -0.2 113,349 + -0.5 113,349 + -1.5 113,349 + +0.7 113,357 - -1.5 113,357 - +1.8 113,357 - -1.4 113,364 + fdhE SO0104 0.10 +0.2 113,428 - fdhE SO0104 0.17 -0.4 113,428 - fdhE SO0104 0.17 +0.3 113,472 + fdhE SO0104 0.22 -0.1 113,583 - fdhE SO0104 0.34 +1.0 113,608 + fdhE SO0104 0.37 -0.3
Or see this region's nucleotide sequence