Experiment: L-Histidine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hutI and hutC are separated by 206 nucleotides hutC and hutU are separated by 126 nucleotides
SO0095: hutI - Imidazolonepropionase (EC 3.5.2.7) (from data) , at 102,534 to 103,760
hutI
SO0096: hutC - histidine utilization repressor (NCBI ptt file), at 103,967 to 104,674
hutC
SO0097: hutU - urocanate hydratase (NCBI ptt file), at 104,801 to 106,471
hutU
Position (kb)
103
104
105 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 102.998 kb on + strand, within hutI at 102.998 kb on + strand, within hutI at 103.025 kb on - strand, within hutI at 103.088 kb on + strand, within hutI at 103.234 kb on + strand, within hutI at 103.234 kb on - strand, within hutI at 103.267 kb on + strand, within hutI at 103.275 kb on - strand, within hutI at 103.275 kb on - strand, within hutI at 103.288 kb on + strand, within hutI at 103.317 kb on + strand, within hutI at 103.343 kb on + strand, within hutI at 103.360 kb on + strand, within hutI at 103.360 kb on + strand, within hutI at 103.360 kb on + strand, within hutI at 103.360 kb on + strand, within hutI at 103.368 kb on - strand, within hutI at 103.428 kb on + strand, within hutI at 103.488 kb on + strand, within hutI at 103.530 kb on + strand, within hutI at 103.532 kb on + strand, within hutI at 103.540 kb on - strand, within hutI at 103.540 kb on - strand, within hutI at 103.556 kb on + strand, within hutI at 103.556 kb on + strand, within hutI at 103.564 kb on - strand, within hutI at 103.564 kb on - strand, within hutI at 103.564 kb on - strand, within hutI at 103.564 kb on - strand, within hutI at 103.564 kb on - strand, within hutI at 103.576 kb on + strand, within hutI at 103.629 kb on + strand, within hutI at 103.634 kb on + strand, within hutI at 103.738 kb on + strand at 103.780 kb on + strand at 103.799 kb on + strand at 103.820 kb on - strand at 103.887 kb on - strand at 103.897 kb on + strand at 103.912 kb on - strand at 104.018 kb on - strand at 104.019 kb on + strand at 104.019 kb on + strand at 104.115 kb on - strand, within hutC at 104.137 kb on + strand, within hutC at 104.145 kb on - strand, within hutC at 104.190 kb on + strand, within hutC at 104.242 kb on + strand, within hutC at 104.242 kb on + strand, within hutC at 104.242 kb on + strand, within hutC at 104.250 kb on - strand, within hutC at 104.250 kb on - strand, within hutC at 104.250 kb on - strand, within hutC at 104.250 kb on - strand, within hutC at 104.275 kb on + strand, within hutC at 104.283 kb on + strand, within hutC at 104.362 kb on + strand, within hutC at 104.387 kb on - strand, within hutC at 104.419 kb on - strand, within hutC at 104.541 kb on - strand, within hutC at 104.562 kb on + strand, within hutC at 104.591 kb on + strand, within hutC at 104.591 kb on + strand, within hutC at 104.599 kb on - strand, within hutC at 104.599 kb on - strand, within hutC at 104.599 kb on - strand, within hutC at 104.704 kb on + strand at 104.773 kb on + strand at 104.789 kb on - strand at 104.817 kb on + strand at 104.817 kb on + strand at 104.825 kb on - strand at 104.840 kb on + strand at 104.885 kb on + strand at 104.908 kb on + strand at 105.052 kb on - strand, within hutU at 105.241 kb on - strand, within hutU at 105.241 kb on - strand, within hutU at 105.260 kb on - strand, within hutU at 105.260 kb on - strand, within hutU at 105.326 kb on + strand, within hutU at 105.334 kb on - strand, within hutU at 105.381 kb on + strand, within hutU at 105.381 kb on + strand, within hutU at 105.382 kb on - strand, within hutU at 105.382 kb on - strand, within hutU at 105.469 kb on - strand, within hutU at 105.567 kb on + strand, within hutU at 105.567 kb on + strand, within hutU at 105.575 kb on - strand, within hutU at 105.592 kb on - strand, within hutU at 105.592 kb on - strand, within hutU at 105.599 kb on - strand, within hutU at 105.628 kb on + strand, within hutU at 105.628 kb on + strand, within hutU at 105.628 kb on + strand, within hutU at 105.636 kb on - strand, within hutU at 105.637 kb on - strand, within hutU
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (N) remove 102,998 + hutI SO0095 0.38 -1.1 102,998 + hutI SO0095 0.38 -3.2 103,025 - hutI SO0095 0.40 -2.0 103,088 + hutI SO0095 0.45 -0.7 103,234 + hutI SO0095 0.57 -1.8 103,234 - hutI SO0095 0.57 -0.0 103,267 + hutI SO0095 0.60 +0.7 103,275 - hutI SO0095 0.60 +0.6 103,275 - hutI SO0095 0.60 -1.7 103,288 + hutI SO0095 0.61 -0.9 103,317 + hutI SO0095 0.64 -0.8 103,343 + hutI SO0095 0.66 -0.5 103,360 + hutI SO0095 0.67 -1.4 103,360 + hutI SO0095 0.67 -2.2 103,360 + hutI SO0095 0.67 -0.7 103,360 + hutI SO0095 0.67 -0.9 103,368 - hutI SO0095 0.68 +0.7 103,428 + hutI SO0095 0.73 -0.7 103,488 + hutI SO0095 0.78 -0.7 103,530 + hutI SO0095 0.81 -1.0 103,532 + hutI SO0095 0.81 +0.5 103,540 - hutI SO0095 0.82 -1.9 103,540 - hutI SO0095 0.82 -1.4 103,556 + hutI SO0095 0.83 +0.3 103,556 + hutI SO0095 0.83 -2.0 103,564 - hutI SO0095 0.84 -0.2 103,564 - hutI SO0095 0.84 +0.9 103,564 - hutI SO0095 0.84 +1.8 103,564 - hutI SO0095 0.84 -0.9 103,564 - hutI SO0095 0.84 -2.3 103,576 + hutI SO0095 0.85 +0.5 103,629 + hutI SO0095 0.89 -2.3 103,634 + hutI SO0095 0.90 -0.1 103,738 + -1.8 103,780 + -0.1 103,799 + -0.2 103,820 - -0.2 103,887 - -1.3 103,897 + -0.8 103,912 - -0.8 104,018 - -1.7 104,019 + -0.2 104,019 + -1.1 104,115 - hutC SO0096 0.21 +1.8 104,137 + hutC SO0096 0.24 -0.6 104,145 - hutC SO0096 0.25 +0.3 104,190 + hutC SO0096 0.31 +0.9 104,242 + hutC SO0096 0.39 +0.2 104,242 + hutC SO0096 0.39 -1.1 104,242 + hutC SO0096 0.39 -0.6 104,250 - hutC SO0096 0.40 -1.4 104,250 - hutC SO0096 0.40 -0.8 104,250 - hutC SO0096 0.40 -1.9 104,250 - hutC SO0096 0.40 -0.3 104,275 + hutC SO0096 0.44 -0.9 104,283 + hutC SO0096 0.45 -1.4 104,362 + hutC SO0096 0.56 +1.6 104,387 - hutC SO0096 0.59 -0.5 104,419 - hutC SO0096 0.64 -1.0 104,541 - hutC SO0096 0.81 -0.9 104,562 + hutC SO0096 0.84 -0.3 104,591 + hutC SO0096 0.88 +0.6 104,591 + hutC SO0096 0.88 +0.8 104,599 - hutC SO0096 0.89 -0.3 104,599 - hutC SO0096 0.89 -1.1 104,599 - hutC SO0096 0.89 +1.2 104,704 + +0.7 104,773 + -1.5 104,789 - -1.4 104,817 + -1.1 104,817 + -0.7 104,825 - -1.8 104,840 + -1.5 104,885 + -1.6 104,908 + -1.0 105,052 - hutU SO0097 0.15 -1.7 105,241 - hutU SO0097 0.26 -0.5 105,241 - hutU SO0097 0.26 +0.1 105,260 - hutU SO0097 0.27 -1.1 105,260 - hutU SO0097 0.27 -1.0 105,326 + hutU SO0097 0.31 -1.2 105,334 - hutU SO0097 0.32 -0.5 105,381 + hutU SO0097 0.35 +0.3 105,381 + hutU SO0097 0.35 -0.0 105,382 - hutU SO0097 0.35 +1.7 105,382 - hutU SO0097 0.35 -1.2 105,469 - hutU SO0097 0.40 -0.9 105,567 + hutU SO0097 0.46 +0.6 105,567 + hutU SO0097 0.46 -0.6 105,575 - hutU SO0097 0.46 -1.2 105,592 - hutU SO0097 0.47 +0.8 105,592 - hutU SO0097 0.47 -0.8 105,599 - hutU SO0097 0.48 +0.4 105,628 + hutU SO0097 0.49 +1.3 105,628 + hutU SO0097 0.49 -1.0 105,628 + hutU SO0097 0.49 +0.3 105,636 - hutU SO0097 0.50 -1.4 105,637 - hutU SO0097 0.50 -0.2
Or see this region's nucleotide sequence