Experiment: L-Histidine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysA-2 and SO4656 are separated by 59 nucleotides SO4656 and SO_4657 overlap by 35 nucleotides SO_4657 and SO4656.2 are separated by 47 nucleotides
SO4655: cysA-2 - sulfate ABC transporter, ATP-binding protein (NCBI ptt file), at 4,850,802 to 4,851,866
cysA-2
SO4656: SO4656 - hypothetical protein (NCBI ptt file), at 4,851,926 to 4,852,510
SO4656
SO_4657: SO_4657 - no description, at 4,852,476 to 4,853,960
_4657
SO4656.2: SO4656.2 - conserved hypothetical protein (NCBI ptt file), at 4,854,008 to 4,856,029
SO4656.2
Position (kb)
4852
4853
4854 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4851.479 kb on - strand, within cysA-2 at 4851.517 kb on + strand, within cysA-2 at 4851.517 kb on + strand, within cysA-2 at 4851.525 kb on - strand, within cysA-2 at 4851.765 kb on + strand at 4851.765 kb on + strand at 4851.768 kb on - strand at 4851.768 kb on - strand at 4851.778 kb on - strand at 4851.815 kb on + strand at 4851.815 kb on + strand at 4851.823 kb on - strand at 4851.853 kb on - strand at 4851.876 kb on - strand at 4851.878 kb on - strand at 4851.914 kb on - strand at 4851.939 kb on + strand at 4851.963 kb on - strand at 4852.053 kb on + strand, within SO4656 at 4852.053 kb on + strand, within SO4656 at 4852.053 kb on + strand, within SO4656 at 4852.053 kb on + strand, within SO4656 at 4852.053 kb on + strand, within SO4656 at 4852.053 kb on + strand, within SO4656 at 4852.053 kb on + strand, within SO4656 at 4852.061 kb on - strand, within SO4656 at 4852.061 kb on - strand, within SO4656 at 4852.061 kb on - strand, within SO4656 at 4852.061 kb on - strand, within SO4656 at 4852.061 kb on - strand, within SO4656 at 4852.061 kb on - strand, within SO4656 at 4852.084 kb on + strand, within SO4656 at 4852.117 kb on - strand, within SO4656 at 4852.117 kb on - strand, within SO4656 at 4852.117 kb on - strand, within SO4656 at 4852.175 kb on + strand, within SO4656 at 4852.299 kb on + strand, within SO4656 at 4852.309 kb on + strand, within SO4656 at 4852.353 kb on + strand, within SO4656 at 4852.361 kb on - strand, within SO4656 at 4852.361 kb on - strand, within SO4656 at 4852.363 kb on - strand, within SO4656 at 4852.371 kb on - strand, within SO4656 at 4852.407 kb on - strand, within SO4656 at 4852.409 kb on + strand, within SO4656 at 4852.409 kb on + strand, within SO4656 at 4852.409 kb on + strand, within SO4656 at 4852.409 kb on + strand, within SO4656 at 4852.409 kb on + strand, within SO4656 at 4852.409 kb on + strand, within SO4656 at 4852.417 kb on - strand, within SO4656 at 4852.417 kb on - strand, within SO4656 at 4852.417 kb on - strand, within SO4656 at 4852.557 kb on + strand at 4852.564 kb on + strand at 4852.580 kb on + strand at 4852.613 kb on + strand at 4852.621 kb on - strand at 4852.708 kb on + strand, within SO_4657 at 4852.709 kb on + strand, within SO_4657 at 4852.709 kb on + strand, within SO_4657 at 4852.717 kb on - strand, within SO_4657 at 4852.717 kb on - strand, within SO_4657 at 4852.717 kb on - strand, within SO_4657 at 4852.717 kb on - strand, within SO_4657 at 4852.837 kb on - strand, within SO_4657 at 4852.911 kb on + strand, within SO_4657 at 4853.078 kb on + strand, within SO_4657 at 4853.147 kb on + strand, within SO_4657 at 4853.147 kb on + strand, within SO_4657 at 4853.170 kb on + strand, within SO_4657 at 4853.217 kb on + strand, within SO_4657 at 4853.217 kb on + strand, within SO_4657 at 4853.224 kb on - strand, within SO_4657 at 4853.225 kb on - strand, within SO_4657 at 4853.225 kb on - strand, within SO_4657 at 4853.225 kb on - strand, within SO_4657 at 4853.225 kb on - strand, within SO_4657 at 4853.239 kb on - strand, within SO_4657 at 4853.343 kb on + strand, within SO_4657 at 4853.345 kb on + strand, within SO_4657 at 4853.369 kb on + strand, within SO_4657 at 4853.400 kb on + strand, within SO_4657 at 4853.400 kb on + strand, within SO_4657 at 4853.400 kb on + strand, within SO_4657 at 4853.400 kb on + strand, within SO_4657 at 4853.408 kb on - strand, within SO_4657 at 4853.433 kb on + strand, within SO_4657 at 4853.473 kb on + strand, within SO_4657 at 4853.475 kb on + strand, within SO_4657 at 4853.475 kb on + strand, within SO_4657 at 4853.475 kb on + strand, within SO_4657 at 4853.475 kb on + strand, within SO_4657 at 4853.483 kb on - strand, within SO_4657 at 4853.483 kb on - strand, within SO_4657 at 4853.523 kb on - strand, within SO_4657 at 4853.523 kb on - strand, within SO_4657 at 4853.545 kb on - strand, within SO_4657 at 4853.611 kb on - strand, within SO_4657 at 4853.611 kb on - strand, within SO_4657 at 4853.661 kb on - strand, within SO_4657 at 4853.703 kb on + strand, within SO_4657 at 4853.714 kb on + strand, within SO_4657 at 4853.714 kb on + strand, within SO_4657 at 4853.800 kb on - strand, within SO_4657 at 4853.931 kb on + strand at 4853.979 kb on - strand at 4854.024 kb on - strand at 4854.024 kb on - strand at 4854.094 kb on + strand at 4854.094 kb on + strand at 4854.102 kb on - strand at 4854.297 kb on - strand, within SO4656.2 at 4854.342 kb on + strand, within SO4656.2 at 4854.342 kb on + strand, within SO4656.2 at 4854.365 kb on - strand, within SO4656.2 at 4854.458 kb on + strand, within SO4656.2 at 4854.458 kb on + strand, within SO4656.2 at 4854.458 kb on + strand, within SO4656.2 at 4854.458 kb on + strand, within SO4656.2 at 4854.466 kb on - strand, within SO4656.2 at 4854.466 kb on - strand, within SO4656.2 at 4854.479 kb on - strand, within SO4656.2 at 4854.505 kb on + strand, within SO4656.2 at 4854.513 kb on - strand, within SO4656.2 at 4854.608 kb on + strand, within SO4656.2 at 4854.616 kb on - strand, within SO4656.2 at 4854.628 kb on + strand, within SO4656.2 at 4854.628 kb on + strand, within SO4656.2 at 4854.639 kb on - strand, within SO4656.2 at 4854.647 kb on + strand, within SO4656.2 at 4854.673 kb on + strand, within SO4656.2 at 4854.681 kb on - strand, within SO4656.2 at 4854.699 kb on + strand, within SO4656.2 at 4854.699 kb on + strand, within SO4656.2 at 4854.742 kb on + strand, within SO4656.2 at 4854.769 kb on - strand, within SO4656.2 at 4854.782 kb on + strand, within SO4656.2 at 4854.839 kb on - strand, within SO4656.2
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (N) remove 4,851,479 - cysA-2 SO4655 0.64 +0.7 4,851,517 + cysA-2 SO4655 0.67 +0.1 4,851,517 + cysA-2 SO4655 0.67 +0.9 4,851,525 - cysA-2 SO4655 0.68 -1.1 4,851,765 + +0.8 4,851,765 + -0.2 4,851,768 - +0.2 4,851,768 - +0.4 4,851,778 - -1.5 4,851,815 + +0.9 4,851,815 + -0.6 4,851,823 - -0.5 4,851,853 - +0.4 4,851,876 - +1.2 4,851,878 - +0.3 4,851,914 - -0.2 4,851,939 + -1.3 4,851,963 - -0.2 4,852,053 + SO4656 0.22 +0.9 4,852,053 + SO4656 0.22 -0.1 4,852,053 + SO4656 0.22 -0.8 4,852,053 + SO4656 0.22 +0.7 4,852,053 + SO4656 0.22 +0.3 4,852,053 + SO4656 0.22 -1.0 4,852,053 + SO4656 0.22 -0.4 4,852,061 - SO4656 0.23 +1.6 4,852,061 - SO4656 0.23 -0.2 4,852,061 - SO4656 0.23 -0.6 4,852,061 - SO4656 0.23 +0.1 4,852,061 - SO4656 0.23 +0.1 4,852,061 - SO4656 0.23 -1.1 4,852,084 + SO4656 0.27 +0.6 4,852,117 - SO4656 0.33 -0.8 4,852,117 - SO4656 0.33 -1.1 4,852,117 - SO4656 0.33 +0.6 4,852,175 + SO4656 0.43 +0.3 4,852,299 + SO4656 0.64 -1.1 4,852,309 + SO4656 0.65 +0.1 4,852,353 + SO4656 0.73 +1.8 4,852,361 - SO4656 0.74 -0.8 4,852,361 - SO4656 0.74 -1.4 4,852,363 - SO4656 0.75 -1.8 4,852,371 - SO4656 0.76 -0.5 4,852,407 - SO4656 0.82 -1.6 4,852,409 + SO4656 0.83 +0.9 4,852,409 + SO4656 0.83 +1.4 4,852,409 + SO4656 0.83 +1.5 4,852,409 + SO4656 0.83 -0.9 4,852,409 + SO4656 0.83 -0.4 4,852,409 + SO4656 0.83 +0.0 4,852,417 - SO4656 0.84 -0.5 4,852,417 - SO4656 0.84 -0.5 4,852,417 - SO4656 0.84 -0.1 4,852,557 + +0.4 4,852,564 + +0.2 4,852,580 + +0.7 4,852,613 + +1.1 4,852,621 - -1.2 4,852,708 + SO_4657 0.16 +1.3 4,852,709 + SO_4657 0.16 +0.8 4,852,709 + SO_4657 0.16 +0.7 4,852,717 - SO_4657 0.16 +0.6 4,852,717 - SO_4657 0.16 -0.6 4,852,717 - SO_4657 0.16 +1.2 4,852,717 - SO_4657 0.16 +0.7 4,852,837 - SO_4657 0.24 +1.5 4,852,911 + SO_4657 0.29 +0.4 4,853,078 + SO_4657 0.41 -0.5 4,853,147 + SO_4657 0.45 +0.5 4,853,147 + SO_4657 0.45 +0.7 4,853,170 + SO_4657 0.47 +0.9 4,853,217 + SO_4657 0.50 -0.3 4,853,217 + SO_4657 0.50 +0.5 4,853,224 - SO_4657 0.50 -0.1 4,853,225 - SO_4657 0.50 -0.3 4,853,225 - SO_4657 0.50 -0.3 4,853,225 - SO_4657 0.50 +0.4 4,853,225 - SO_4657 0.50 -1.1 4,853,239 - SO_4657 0.51 +0.9 4,853,343 + SO_4657 0.58 +2.0 4,853,345 + SO_4657 0.59 +0.2 4,853,369 + SO_4657 0.60 +1.3 4,853,400 + SO_4657 0.62 +0.6 4,853,400 + SO_4657 0.62 +1.2 4,853,400 + SO_4657 0.62 +0.6 4,853,400 + SO_4657 0.62 +1.0 4,853,408 - SO_4657 0.63 +0.5 4,853,433 + SO_4657 0.64 +0.9 4,853,473 + SO_4657 0.67 +1.4 4,853,475 + SO_4657 0.67 -1.2 4,853,475 + SO_4657 0.67 +0.4 4,853,475 + SO_4657 0.67 +0.4 4,853,475 + SO_4657 0.67 +0.9 4,853,483 - SO_4657 0.68 +1.5 4,853,483 - SO_4657 0.68 +0.5 4,853,523 - SO_4657 0.71 -0.3 4,853,523 - SO_4657 0.71 +1.5 4,853,545 - SO_4657 0.72 +0.5 4,853,611 - SO_4657 0.76 -0.1 4,853,611 - SO_4657 0.76 +0.7 4,853,661 - SO_4657 0.80 -0.7 4,853,703 + SO_4657 0.83 +1.5 4,853,714 + SO_4657 0.83 +1.1 4,853,714 + SO_4657 0.83 +0.8 4,853,800 - SO_4657 0.89 +1.4 4,853,931 + +0.6 4,853,979 - +0.3 4,854,024 - +0.8 4,854,024 - +0.5 4,854,094 + -0.2 4,854,094 + +0.8 4,854,102 - +1.6 4,854,297 - SO4656.2 0.14 -0.4 4,854,342 + SO4656.2 0.17 +0.5 4,854,342 + SO4656.2 0.17 -0.8 4,854,365 - SO4656.2 0.18 +0.0 4,854,458 + SO4656.2 0.22 +0.4 4,854,458 + SO4656.2 0.22 -1.6 4,854,458 + SO4656.2 0.22 +0.1 4,854,458 + SO4656.2 0.22 +0.8 4,854,466 - SO4656.2 0.23 -0.0 4,854,466 - SO4656.2 0.23 +0.1 4,854,479 - SO4656.2 0.23 +0.2 4,854,505 + SO4656.2 0.25 +0.0 4,854,513 - SO4656.2 0.25 +0.4 4,854,608 + SO4656.2 0.30 -0.1 4,854,616 - SO4656.2 0.30 +1.8 4,854,628 + SO4656.2 0.31 +0.0 4,854,628 + SO4656.2 0.31 +0.2 4,854,639 - SO4656.2 0.31 +1.1 4,854,647 + SO4656.2 0.32 -0.4 4,854,673 + SO4656.2 0.33 +0.9 4,854,681 - SO4656.2 0.33 +0.4 4,854,699 + SO4656.2 0.34 +0.1 4,854,699 + SO4656.2 0.34 +0.1 4,854,742 + SO4656.2 0.36 +1.2 4,854,769 - SO4656.2 0.38 +0.8 4,854,782 + SO4656.2 0.38 +0.8 4,854,839 - SO4656.2 0.41 -0.6
Or see this region's nucleotide sequence