Experiment: Def Cisplatin 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0058 and Psest_0059 are separated by 389 nucleotides Psest_0059 and Psest_0060 are separated by 2 nucleotides Psest_0060 and Psest_0061 are separated by 118 nucleotides Psest_0061 and Psest_0062 are separated by 175 nucleotides
Psest_0058: Psest_0058 - HipA-like C-terminal domain./HipA-like N-terminal domain., at 59,073 to 60,392
_0058
Psest_0059: Psest_0059 - hypothetical protein, at 60,782 to 61,153
_0059
Psest_0060: Psest_0060 - Predicted Fe-S protein, at 61,156 to 61,338
_0060
Psest_0061: Psest_0061 - hypothetical protein, at 61,457 to 61,786
_0061
Psest_0062: Psest_0062 - tryptophan synthase, alpha subunit, at 61,962 to 62,771
_0062
Position (kb) 61
62 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 60.158 kb on - strand, within Psest_0058 at 60.185 kb on - strand, within Psest_0058 at 60.185 kb on - strand, within Psest_0058 at 60.191 kb on + strand, within Psest_0058 at 60.268 kb on + strand at 60.268 kb on + strand at 60.269 kb on - strand at 60.269 kb on - strand at 60.269 kb on - strand at 60.385 kb on + strand at 60.541 kb on + strand at 60.541 kb on + strand at 60.542 kb on - strand at 60.545 kb on + strand at 60.576 kb on + strand at 60.577 kb on - strand at 60.666 kb on + strand at 60.666 kb on + strand at 60.667 kb on - strand at 60.667 kb on - strand at 60.726 kb on - strand at 60.905 kb on + strand, within Psest_0059 at 61.215 kb on + strand, within Psest_0060 at 61.216 kb on - strand, within Psest_0060 at 61.290 kb on - strand, within Psest_0060 at 61.416 kb on + strand at 61.417 kb on - strand at 61.530 kb on + strand, within Psest_0061 at 61.530 kb on + strand, within Psest_0061 at 61.530 kb on + strand, within Psest_0061 at 61.531 kb on - strand, within Psest_0061 at 61.641 kb on + strand, within Psest_0061 at 61.653 kb on + strand, within Psest_0061 at 61.896 kb on + strand at 61.897 kb on - strand at 61.900 kb on + strand at 61.900 kb on + strand at 61.900 kb on + strand at 61.901 kb on - strand at 61.901 kb on - strand at 61.901 kb on - strand at 61.901 kb on - strand at 61.901 kb on - strand at 61.980 kb on + strand at 61.980 kb on + strand at 61.981 kb on - strand at 62.245 kb on + strand, within Psest_0062 at 62.245 kb on + strand, within Psest_0062 at 62.245 kb on + strand, within Psest_0062 at 62.245 kb on + strand, within Psest_0062 at 62.246 kb on - strand, within Psest_0062 at 62.246 kb on - strand, within Psest_0062 at 62.246 kb on - strand, within Psest_0062 at 62.246 kb on - strand, within Psest_0062 at 62.246 kb on - strand, within Psest_0062 at 62.248 kb on + strand, within Psest_0062 at 62.249 kb on - strand, within Psest_0062 at 62.249 kb on - strand, within Psest_0062 at 62.249 kb on - strand, within Psest_0062
Per-strain Table
Position Strand Gene LocusTag Fraction Def Cisplatin 0.0002 mg/ml remove 60,158 - Psest_0058 0.82 -0.1 60,185 - Psest_0058 0.84 -0.3 60,185 - Psest_0058 0.84 -0.9 60,191 + Psest_0058 0.85 -0.9 60,268 + +0.4 60,268 + -0.4 60,269 - -0.8 60,269 - -2.9 60,269 - +2.4 60,385 + +0.8 60,541 + +1.7 60,541 + +0.7 60,542 - +0.2 60,545 + -0.7 60,576 + +0.2 60,577 - -1.4 60,666 + +0.2 60,666 + -0.1 60,667 - -0.3 60,667 - -0.5 60,726 - -0.3 60,905 + Psest_0059 0.33 -0.9 61,215 + Psest_0060 0.32 -0.2 61,216 - Psest_0060 0.33 -1.0 61,290 - Psest_0060 0.73 -2.3 61,416 + +0.2 61,417 - -0.3 61,530 + Psest_0061 0.22 -1.5 61,530 + Psest_0061 0.22 -0.5 61,530 + Psest_0061 0.22 +2.4 61,531 - Psest_0061 0.22 -0.2 61,641 + Psest_0061 0.56 -0.5 61,653 + Psest_0061 0.59 +0.9 61,896 + -0.5 61,897 - +0.3 61,900 + +0.4 61,900 + +0.5 61,900 + +0.1 61,901 - -0.6 61,901 - -1.4 61,901 - -0.0 61,901 - +0.6 61,901 - +0.9 61,980 + -0.3 61,980 + +1.8 61,981 - +1.3 62,245 + Psest_0062 0.35 -3.4 62,245 + Psest_0062 0.35 -4.7 62,245 + Psest_0062 0.35 -3.4 62,245 + Psest_0062 0.35 -3.2 62,246 - Psest_0062 0.35 +0.1 62,246 - Psest_0062 0.35 -3.6 62,246 - Psest_0062 0.35 -1.7 62,246 - Psest_0062 0.35 -0.9 62,246 - Psest_0062 0.35 -2.4 62,248 + Psest_0062 0.35 -4.2 62,249 - Psest_0062 0.35 -1.3 62,249 - Psest_0062 0.35 -0.9 62,249 - Psest_0062 0.35 -2.1
Or see this region's nucleotide sequence