Experiment: Def Cisplatin 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2283 and Psest_2284 are separated by 205 nucleotides Psest_2284 and Psest_2285 are separated by 130 nucleotides Psest_2285 and Psest_2286 are separated by 133 nucleotides
Psest_2283: Psest_2283 - Parvulin-like peptidyl-prolyl isomerase, at 2,431,300 to 2,433,156
_2283
Psest_2284: Psest_2284 - Bacterial nucleoid DNA-binding protein, at 2,433,362 to 2,433,634
_2284
Psest_2285: Psest_2285 - ATP-dependent protease La, at 2,433,765 to 2,436,161
_2285
Psest_2286: Psest_2286 - endopeptidase Clp ATP-binding regulatory subunit (clpX), at 2,436,295 to 2,437,575
_2286
Position (kb)
2433
2434
2435
2436
2437 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2433.364 kb on - strand at 2433.364 kb on - strand at 2433.364 kb on - strand at 2433.683 kb on - strand at 2433.683 kb on - strand at 2433.698 kb on - strand at 2433.706 kb on - strand at 2433.767 kb on - strand at 2433.860 kb on - strand at 2434.186 kb on - strand, within Psest_2285 at 2434.186 kb on - strand, within Psest_2285 at 2434.186 kb on - strand, within Psest_2285 at 2434.434 kb on + strand, within Psest_2285 at 2434.434 kb on + strand, within Psest_2285 at 2434.436 kb on + strand, within Psest_2285 at 2434.673 kb on + strand, within Psest_2285 at 2434.808 kb on - strand, within Psest_2285 at 2434.981 kb on + strand, within Psest_2285 at 2434.981 kb on + strand, within Psest_2285 at 2434.982 kb on - strand, within Psest_2285 at 2435.293 kb on + strand, within Psest_2285 at 2435.293 kb on + strand, within Psest_2285 at 2435.294 kb on - strand, within Psest_2285 at 2435.488 kb on + strand, within Psest_2285 at 2435.608 kb on - strand, within Psest_2285 at 2435.969 kb on - strand at 2436.162 kb on - strand at 2436.162 kb on - strand at 2436.173 kb on - strand at 2436.339 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Cisplatin 0.0002 mg/ml remove 2,433,364 - -0.4 2,433,364 - +0.1 2,433,364 - -1.1 2,433,683 - +0.0 2,433,683 - +1.4 2,433,698 - +0.6 2,433,706 - -0.3 2,433,767 - +1.1 2,433,860 - -0.2 2,434,186 - Psest_2285 0.18 -2.1 2,434,186 - Psest_2285 0.18 -2.8 2,434,186 - Psest_2285 0.18 -2.9 2,434,434 + Psest_2285 0.28 -0.3 2,434,434 + Psest_2285 0.28 -0.9 2,434,436 + Psest_2285 0.28 -2.2 2,434,673 + Psest_2285 0.38 -0.1 2,434,808 - Psest_2285 0.44 -0.9 2,434,981 + Psest_2285 0.51 -0.4 2,434,981 + Psest_2285 0.51 -1.3 2,434,982 - Psest_2285 0.51 -1.6 2,435,293 + Psest_2285 0.64 -1.8 2,435,293 + Psest_2285 0.64 +2.9 2,435,294 - Psest_2285 0.64 -0.9 2,435,488 + Psest_2285 0.72 -1.4 2,435,608 - Psest_2285 0.77 -1.0 2,435,969 - -1.1 2,436,162 - +0.3 2,436,162 - +0.5 2,436,173 - -0.1 2,436,339 - -0.9
Or see this region's nucleotide sequence