Experiment: Def Cisplatin 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0213 and Psest_0214 are separated by 70 nucleotides Psest_0214 and Psest_0215 are separated by 130 nucleotides Psest_0215 and Psest_0216 are separated by 0 nucleotides
Psest_0213: Psest_0213 - exodeoxyribonuclease V, beta subunit, at 216,625 to 220,173
_0213
Psest_0214: Psest_0214 - exodeoxyribonuclease V, alpha subunit, at 220,244 to 222,091
_0214
Psest_0215: Psest_0215 - hypothetical protein, at 222,222 to 222,665
_0215
Psest_0216: Psest_0216 - Predicted permeases, at 222,666 to 223,574
_0216
Position (kb) 220
221
222
223 Strain fitness (log2 ratio) -2
-1
0
1
2 at 220.241 kb on + strand at 220.241 kb on + strand at 220.241 kb on + strand at 220.241 kb on + strand at 220.242 kb on - strand at 220.243 kb on + strand at 220.243 kb on + strand at 220.243 kb on + strand at 220.243 kb on + strand at 220.243 kb on + strand at 220.243 kb on + strand at 220.243 kb on + strand at 220.243 kb on + strand at 220.243 kb on + strand at 220.243 kb on + strand at 220.243 kb on + strand at 220.243 kb on + strand at 220.243 kb on + strand at 220.243 kb on + strand at 220.243 kb on + strand at 220.243 kb on + strand at 220.243 kb on + strand at 220.244 kb on - strand at 220.244 kb on - strand at 220.244 kb on - strand at 220.244 kb on - strand at 220.244 kb on - strand at 220.244 kb on - strand at 220.244 kb on - strand at 220.244 kb on - strand at 220.244 kb on - strand at 220.244 kb on - strand at 220.244 kb on - strand at 220.244 kb on - strand at 220.244 kb on - strand at 220.244 kb on - strand at 220.244 kb on - strand at 220.244 kb on - strand at 220.244 kb on - strand at 221.888 kb on + strand, within Psest_0214 at 222.244 kb on + strand at 222.245 kb on - strand at 222.287 kb on + strand, within Psest_0215 at 222.342 kb on + strand, within Psest_0215 at 222.342 kb on + strand, within Psest_0215 at 222.343 kb on - strand, within Psest_0215 at 222.343 kb on - strand, within Psest_0215 at 222.343 kb on - strand, within Psest_0215 at 222.345 kb on + strand, within Psest_0215 at 222.345 kb on + strand, within Psest_0215 at 222.345 kb on + strand, within Psest_0215 at 222.346 kb on - strand, within Psest_0215 at 222.346 kb on - strand, within Psest_0215 at 222.346 kb on - strand, within Psest_0215 at 222.346 kb on - strand, within Psest_0215 at 222.346 kb on - strand, within Psest_0215 at 222.346 kb on - strand, within Psest_0215 at 222.346 kb on - strand, within Psest_0215 at 222.346 kb on - strand, within Psest_0215 at 222.346 kb on - strand, within Psest_0215 at 222.382 kb on - strand, within Psest_0215 at 222.484 kb on + strand, within Psest_0215 at 222.501 kb on + strand, within Psest_0215 at 222.781 kb on + strand, within Psest_0216 at 222.781 kb on + strand at 222.781 kb on + strand, within Psest_0216 at 222.782 kb on - strand, within Psest_0216 at 222.937 kb on - strand, within Psest_0216
Per-strain Table
Position Strand Gene LocusTag Fraction Def Cisplatin 0.0002 mg/ml remove 220,241 + +0.9 220,241 + +1.1 220,241 + +0.1 220,241 + -0.2 220,242 - -1.9 220,243 + -0.9 220,243 + +1.1 220,243 + +0.4 220,243 + +1.1 220,243 + -1.5 220,243 + +0.7 220,243 + -0.5 220,243 + +0.8 220,243 + -1.2 220,243 + +1.7 220,243 + -0.5 220,243 + +1.4 220,243 + +0.6 220,243 + -0.5 220,243 + -0.1 220,243 + +1.1 220,243 + +0.6 220,244 - -1.1 220,244 - -0.1 220,244 - +0.1 220,244 - -0.9 220,244 - -0.7 220,244 - +0.7 220,244 - -0.1 220,244 - -0.7 220,244 - +1.1 220,244 - -1.2 220,244 - -0.2 220,244 - -1.5 220,244 - +1.1 220,244 - +0.1 220,244 - +0.8 220,244 - +1.4 220,244 - -0.9 221,888 + Psest_0214 0.89 -0.9 222,244 + +0.4 222,245 - +0.5 222,287 + Psest_0215 0.15 +1.3 222,342 + Psest_0215 0.27 -0.5 222,342 + Psest_0215 0.27 +0.1 222,343 - Psest_0215 0.27 +0.1 222,343 - Psest_0215 0.27 -0.9 222,343 - Psest_0215 0.27 -0.5 222,345 + Psest_0215 0.28 +0.1 222,345 + Psest_0215 0.28 +2.1 222,345 + Psest_0215 0.28 +0.1 222,346 - Psest_0215 0.28 -0.9 222,346 - Psest_0215 0.28 -0.2 222,346 - Psest_0215 0.28 +0.6 222,346 - Psest_0215 0.28 +1.8 222,346 - Psest_0215 0.28 +0.1 222,346 - Psest_0215 0.28 -1.1 222,346 - Psest_0215 0.28 +0.5 222,346 - Psest_0215 0.28 +2.7 222,346 - Psest_0215 0.28 +0.7 222,382 - Psest_0215 0.36 +1.1 222,484 + Psest_0215 0.59 +0.9 222,501 + Psest_0215 0.63 +1.1 222,781 + Psest_0216 0.13 +1.1 222,781 + +1.1 222,781 + Psest_0216 0.13 +0.7 222,782 - Psest_0216 0.13 +2.1 222,937 - Psest_0216 0.30 -0.5
Or see this region's nucleotide sequence