Experiment: Def Cisplatin 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0167 and Psest_0168 are separated by 1 nucleotides Psest_0168 and Psest_0169 are separated by 197 nucleotides Psest_0169 and Psest_0170 are separated by 28 nucleotides Psest_0170 and Psest_0172 are separated by 218 nucleotides Psest_0172 and Psest_0173 overlap by 4 nucleotides Psest_0173 and Psest_0174 are separated by 129 nucleotides
Psest_0167: Psest_0167 - Flagellar basal body-associated protein, at 164,782 to 165,183
_0167
Psest_0168: Psest_0168 - Uncharacterized conserved protein, at 165,185 to 165,634
_0168
Psest_0169: Psest_0169 - hypothetical protein, at 165,832 to 165,984
_0169
Psest_0170: Psest_0170 - 5,10-methenyltetrahydrofolate synthetase, at 166,013 to 166,615
_0170
Psest_0172: Psest_0172 - Uncharacterized protein conserved in bacteria, at 166,834 to 167,148
_0172
Psest_0173: Psest_0173 - TIGR02449 family protein, at 167,145 to 167,354
_0173
Psest_0174: Psest_0174 - Uncharacterized protein conserved in bacteria, at 167,484 to 168,038
_0174
Position (kb) 166
167 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 165.135 kb on + strand, within Psest_0167 at 165.136 kb on - strand, within Psest_0167 at 165.486 kb on + strand, within Psest_0168 at 165.486 kb on + strand, within Psest_0168 at 165.487 kb on - strand, within Psest_0168 at 165.487 kb on - strand, within Psest_0168 at 165.487 kb on - strand, within Psest_0168 at 165.487 kb on - strand, within Psest_0168 at 165.487 kb on - strand, within Psest_0168 at 165.514 kb on + strand, within Psest_0168 at 165.514 kb on + strand, within Psest_0168 at 165.514 kb on + strand, within Psest_0168 at 165.515 kb on - strand, within Psest_0168 at 165.515 kb on - strand, within Psest_0168 at 165.541 kb on - strand, within Psest_0168 at 165.549 kb on - strand, within Psest_0168 at 165.627 kb on + strand at 165.703 kb on + strand at 165.703 kb on + strand at 165.703 kb on + strand at 165.704 kb on - strand at 165.704 kb on - strand at 165.704 kb on - strand at 165.750 kb on - strand at 165.750 kb on - strand at 165.769 kb on + strand at 165.769 kb on + strand at 165.777 kb on + strand at 165.777 kb on + strand at 165.840 kb on + strand at 165.840 kb on + strand at 165.840 kb on + strand at 165.840 kb on + strand at 165.841 kb on - strand at 165.849 kb on + strand, within Psest_0169 at 165.903 kb on + strand, within Psest_0169 at 166.023 kb on + strand at 166.023 kb on + strand at 166.058 kb on + strand at 166.058 kb on + strand at 166.058 kb on + strand at 166.059 kb on - strand at 166.059 kb on - strand at 166.059 kb on - strand at 166.059 kb on - strand at 166.059 kb on - strand at 166.173 kb on + strand, within Psest_0170 at 166.174 kb on - strand, within Psest_0170 at 166.174 kb on - strand, within Psest_0170 at 166.174 kb on - strand, within Psest_0170 at 166.174 kb on - strand, within Psest_0170 at 166.174 kb on - strand, within Psest_0170 at 166.174 kb on - strand, within Psest_0170 at 166.174 kb on - strand, within Psest_0170 at 166.174 kb on - strand, within Psest_0170 at 166.383 kb on + strand, within Psest_0170 at 166.383 kb on + strand, within Psest_0170 at 166.383 kb on + strand, within Psest_0170 at 166.383 kb on + strand, within Psest_0170 at 166.383 kb on + strand, within Psest_0170 at 166.384 kb on - strand, within Psest_0170 at 166.384 kb on - strand, within Psest_0170 at 166.384 kb on - strand, within Psest_0170 at 166.456 kb on - strand, within Psest_0170 at 166.456 kb on - strand, within Psest_0170 at 166.456 kb on - strand, within Psest_0170 at 166.649 kb on + strand at 166.650 kb on - strand at 166.683 kb on + strand at 166.695 kb on + strand at 166.696 kb on - strand at 166.696 kb on - strand at 166.731 kb on + strand at 166.731 kb on + strand at 166.731 kb on + strand at 166.731 kb on + strand at 166.731 kb on + strand at 166.732 kb on - strand at 166.732 kb on - strand at 166.732 kb on - strand at 166.732 kb on - strand at 166.737 kb on + strand at 166.737 kb on + strand at 166.749 kb on + strand at 166.750 kb on - strand at 166.809 kb on + strand at 166.809 kb on + strand at 166.809 kb on + strand at 166.809 kb on + strand at 166.810 kb on - strand at 166.810 kb on - strand at 166.810 kb on - strand at 166.810 kb on - strand at 166.810 kb on - strand at 166.897 kb on + strand, within Psest_0172 at 166.897 kb on + strand, within Psest_0172 at 166.897 kb on + strand, within Psest_0172 at 166.897 kb on + strand, within Psest_0172 at 166.897 kb on + strand, within Psest_0172 at 166.897 kb on + strand, within Psest_0172 at 166.897 kb on + strand, within Psest_0172 at 166.897 kb on + strand, within Psest_0172 at 166.897 kb on + strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 167.102 kb on + strand, within Psest_0172 at 167.102 kb on + strand, within Psest_0172 at 167.102 kb on + strand, within Psest_0172 at 167.103 kb on - strand at 167.103 kb on - strand, within Psest_0172 at 167.103 kb on - strand, within Psest_0172 at 167.433 kb on - strand at 167.433 kb on - strand at 167.454 kb on - strand at 167.454 kb on - strand at 167.454 kb on - strand at 167.454 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Cisplatin 0.0002 mg/ml remove 165,135 + Psest_0167 0.88 -0.8 165,136 - Psest_0167 0.88 -1.1 165,486 + Psest_0168 0.67 -0.3 165,486 + Psest_0168 0.67 +0.7 165,487 - Psest_0168 0.67 +0.2 165,487 - Psest_0168 0.67 -0.8 165,487 - Psest_0168 0.67 -1.1 165,487 - Psest_0168 0.67 +0.1 165,487 - Psest_0168 0.67 -0.4 165,514 + Psest_0168 0.73 -0.0 165,514 + Psest_0168 0.73 +0.1 165,514 + Psest_0168 0.73 -0.8 165,515 - Psest_0168 0.73 +0.1 165,515 - Psest_0168 0.73 +1.3 165,541 - Psest_0168 0.79 +0.3 165,549 - Psest_0168 0.81 +0.5 165,627 + +0.7 165,703 + +1.9 165,703 + +0.4 165,703 + -0.3 165,704 - -0.9 165,704 - -1.2 165,704 - -1.2 165,750 - -1.4 165,750 - -0.8 165,769 + -1.7 165,769 + -0.8 165,777 + -0.3 165,777 + -1.4 165,840 + -1.0 165,840 + -1.7 165,840 + -0.5 165,840 + -0.9 165,841 - -1.5 165,849 + Psest_0169 0.11 +1.5 165,903 + Psest_0169 0.46 -0.5 166,023 + -1.7 166,023 + -0.5 166,058 + -2.2 166,058 + -0.8 166,058 + -2.2 166,059 - -1.4 166,059 - -1.5 166,059 - -0.8 166,059 - +0.1 166,059 - -1.4 166,173 + Psest_0170 0.27 -2.2 166,174 - Psest_0170 0.27 -0.6 166,174 - Psest_0170 0.27 -3.0 166,174 - Psest_0170 0.27 -0.9 166,174 - Psest_0170 0.27 -1.1 166,174 - Psest_0170 0.27 -0.6 166,174 - Psest_0170 0.27 -3.0 166,174 - Psest_0170 0.27 -2.4 166,174 - Psest_0170 0.27 -1.1 166,383 + Psest_0170 0.61 -1.5 166,383 + Psest_0170 0.61 -0.5 166,383 + Psest_0170 0.61 -0.2 166,383 + Psest_0170 0.61 -3.1 166,383 + Psest_0170 0.61 -2.6 166,384 - Psest_0170 0.62 -0.5 166,384 - Psest_0170 0.62 -0.2 166,384 - Psest_0170 0.62 -2.8 166,456 - Psest_0170 0.73 -0.5 166,456 - Psest_0170 0.73 -1.5 166,456 - Psest_0170 0.73 -2.8 166,649 + -0.9 166,650 - +0.2 166,683 + -0.5 166,695 + +0.1 166,696 - -1.6 166,696 - -0.2 166,731 + -0.3 166,731 + -1.2 166,731 + -0.0 166,731 + -0.6 166,731 + -0.2 166,732 - +1.8 166,732 - -0.4 166,732 - -0.3 166,732 - +0.3 166,737 + +0.3 166,737 + -0.7 166,749 + -1.2 166,750 - +1.0 166,809 + +0.2 166,809 + +0.7 166,809 + -0.5 166,809 + -1.7 166,810 - -0.6 166,810 - +1.1 166,810 - -0.1 166,810 - -0.2 166,810 - -0.5 166,897 + Psest_0172 0.20 -0.5 166,897 + Psest_0172 0.20 -0.8 166,897 + Psest_0172 0.20 +0.2 166,897 + Psest_0172 0.20 +0.1 166,897 + Psest_0172 0.20 +0.3 166,897 + Psest_0172 0.20 -0.2 166,897 + Psest_0172 0.20 -0.3 166,897 + Psest_0172 0.20 -0.5 166,897 + Psest_0172 0.20 -0.7 166,898 - Psest_0172 0.20 +0.6 166,898 - Psest_0172 0.20 +0.0 166,898 - Psest_0172 0.20 +0.3 166,898 - Psest_0172 0.20 +1.1 166,898 - Psest_0172 0.20 +0.4 166,898 - Psest_0172 0.20 +0.6 166,898 - Psest_0172 0.20 -0.7 166,898 - Psest_0172 0.20 -0.1 166,898 - Psest_0172 0.20 -0.5 166,898 - Psest_0172 0.20 -1.9 166,898 - Psest_0172 0.20 -0.3 167,102 + Psest_0172 0.85 -0.0 167,102 + Psest_0172 0.85 -0.8 167,102 + Psest_0172 0.85 +0.2 167,103 - -0.9 167,103 - Psest_0172 0.85 -0.7 167,103 - Psest_0172 0.85 -0.7 167,433 - +0.1 167,433 - -0.0 167,454 - -1.1 167,454 - -1.3 167,454 - -0.7 167,454 - -0.8
Or see this region's nucleotide sequence