Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysJ and pntA are separated by 416 nucleotides pntA and pntB are separated by 11 nucleotides
SO3738: cysJ - sulfite reductase (NADPH) flavoprotein alpha-component (NCBI ptt file), at 3,882,734 to 3,884,557
cysJ
SO3740: pntA - NAD(P) transhydrogenase, alpha subunit (NCBI ptt file), at 3,884,974 to 3,886,500
pntA
SO3741: pntB - NAD(P) transhydrogenase, beta subunit (NCBI ptt file), at 3,886,512 to 3,887,921
pntB
Position (kb)
3884
3885
3886
3887 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3884.257 kb on - strand, within cysJ at 3884.257 kb on - strand, within cysJ at 3884.285 kb on - strand, within cysJ at 3884.373 kb on + strand, within cysJ at 3884.498 kb on - strand at 3884.577 kb on + strand at 3884.691 kb on + strand at 3884.729 kb on + strand at 3884.795 kb on - strand at 3884.900 kb on + strand at 3884.900 kb on + strand at 3884.900 kb on + strand at 3884.900 kb on + strand at 3884.900 kb on + strand at 3884.900 kb on + strand at 3884.908 kb on - strand at 3884.908 kb on - strand at 3884.908 kb on - strand at 3884.908 kb on - strand at 3884.908 kb on - strand at 3884.921 kb on + strand at 3884.966 kb on - strand at 3885.038 kb on + strand at 3885.046 kb on - strand at 3885.062 kb on + strand at 3885.070 kb on - strand at 3885.141 kb on + strand, within pntA at 3885.153 kb on + strand, within pntA at 3885.229 kb on + strand, within pntA at 3885.239 kb on + strand, within pntA at 3885.327 kb on - strand, within pntA at 3885.344 kb on + strand, within pntA at 3885.677 kb on - strand, within pntA at 3885.705 kb on + strand, within pntA at 3885.705 kb on + strand, within pntA at 3885.774 kb on - strand, within pntA at 3885.986 kb on - strand, within pntA at 3886.035 kb on - strand, within pntA at 3886.060 kb on + strand, within pntA at 3886.318 kb on + strand, within pntA at 3886.326 kb on - strand, within pntA at 3886.326 kb on - strand, within pntA at 3886.387 kb on + strand at 3886.405 kb on + strand at 3886.407 kb on + strand at 3886.436 kb on + strand at 3886.460 kb on + strand at 3886.494 kb on + strand at 3886.526 kb on + strand at 3886.544 kb on - strand at 3886.781 kb on + strand, within pntB at 3886.786 kb on - strand, within pntB at 3886.864 kb on + strand, within pntB at 3886.906 kb on - strand, within pntB at 3887.027 kb on + strand, within pntB at 3887.053 kb on + strand, within pntB at 3887.116 kb on + strand, within pntB at 3887.297 kb on + strand, within pntB at 3887.309 kb on + strand, within pntB
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 3,884,257 - cysJ SO3738 0.83 -2.6 3,884,257 - cysJ SO3738 0.83 -3.2 3,884,285 - cysJ SO3738 0.85 -2.9 3,884,373 + cysJ SO3738 0.90 -1.9 3,884,498 - -4.0 3,884,577 + -3.0 3,884,691 + -1.9 3,884,729 + -2.0 3,884,795 - -2.1 3,884,900 + -3.1 3,884,900 + -1.3 3,884,900 + -3.2 3,884,900 + -4.6 3,884,900 + -1.5 3,884,900 + -2.3 3,884,908 - -4.4 3,884,908 - -2.1 3,884,908 - -4.2 3,884,908 - -4.2 3,884,908 - -3.9 3,884,921 + -2.5 3,884,966 - -2.0 3,885,038 + -3.3 3,885,046 - -2.0 3,885,062 + -3.0 3,885,070 - -3.4 3,885,141 + pntA SO3740 0.11 -3.6 3,885,153 + pntA SO3740 0.12 -1.3 3,885,229 + pntA SO3740 0.17 -2.9 3,885,239 + pntA SO3740 0.17 -2.8 3,885,327 - pntA SO3740 0.23 -3.8 3,885,344 + pntA SO3740 0.24 -4.8 3,885,677 - pntA SO3740 0.46 -2.6 3,885,705 + pntA SO3740 0.48 -2.5 3,885,705 + pntA SO3740 0.48 -1.7 3,885,774 - pntA SO3740 0.52 -3.3 3,885,986 - pntA SO3740 0.66 -3.1 3,886,035 - pntA SO3740 0.69 -4.2 3,886,060 + pntA SO3740 0.71 -3.2 3,886,318 + pntA SO3740 0.88 -3.2 3,886,326 - pntA SO3740 0.89 -2.9 3,886,326 - pntA SO3740 0.89 -2.2 3,886,387 + -2.5 3,886,405 + -3.6 3,886,407 + +0.0 3,886,436 + -4.1 3,886,460 + -2.1 3,886,494 + -2.2 3,886,526 + -4.5 3,886,544 - -3.8 3,886,781 + pntB SO3741 0.19 -3.7 3,886,786 - pntB SO3741 0.19 -2.3 3,886,864 + pntB SO3741 0.25 -2.1 3,886,906 - pntB SO3741 0.28 -2.4 3,887,027 + pntB SO3741 0.37 -3.0 3,887,053 + pntB SO3741 0.38 -2.8 3,887,116 + pntB SO3741 0.43 -3.9 3,887,297 + pntB SO3741 0.56 -3.7 3,887,309 + pntB SO3741 0.57 -5.4
Or see this region's nucleotide sequence